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However, to be appropriately used, these techniques require mycological expertise that is not widely available and may be absent in peripheral health care facilities in endemic areas. In addition, they lack sensitivity and specificity, and the culture for isolation and identification can have a long time-to-results period. Molecular methods, including matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS), have been developed in well-equipped reference laboratories. They greatly improve the rapidity and accuracy of diagnosis; in particular, for species identification. Recently, PCR and sequencing have paved the way for more user-friendly point-of-care tests, such as those based on LAMP or RCA technologies, which can be used in basic healthcare settings and even in field consultations.<\/jats:p>","DOI":"10.3390\/jof8040382","type":"journal-article","created":{"date-parts":[[2022,4,10]],"date-time":"2022-04-10T06:02:54Z","timestamp":1649570574000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Molecular Diagnosis of Two Major Implantation Mycoses: Chromoblastomycosis and Sporotrichosis"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9080-7638","authenticated-orcid":false,"given":"Dani\u00e8le","family":"Maubon","sequence":"first","affiliation":[{"name":"Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Universit\u00e9 Grenoble Alpes, Domaine de la Merci, Centre Hospitalier Universitaire Grenoble Alpes, Service de Parasitologie-Mycologie, Bd de la Chantourne, CEDEX, 38706 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Garnaud","sequence":"additional","affiliation":[{"name":"Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Universit\u00e9 Grenoble Alpes, Domaine de la Merci, Centre Hospitalier Universitaire Grenoble Alpes, Service de Parasitologie-Mycologie, Bd de la Chantourne, CEDEX, 38706 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lala Soavina","family":"Ramarozatovo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Hospital Joseph Raseta Befelatanana, Antananarivo 101, Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapelanoro Rabenja","family":"Fahafahantsoa","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Hospital Joseph Raseta Befelatanana, Antananarivo 101, Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4155-3675","authenticated-orcid":false,"given":"Muriel","family":"Cornet","sequence":"additional","affiliation":[{"name":"Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Universit\u00e9 Grenoble Alpes, Domaine de la Merci, Centre Hospitalier Universitaire Grenoble Alpes, Service de Parasitologie-Mycologie, Bd de la Chantourne, CEDEX, 38706 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3223-3277","authenticated-orcid":false,"given":"Tahinamandranto","family":"Rasamoelina","sequence":"additional","affiliation":[{"name":"Centre d\u2019Infectiologie Charles M\u00e9rieux, Universit\u00e9 d\u2019Antananarivo, Antananarivo 101, Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1128\/CMR.00032-16","article-title":"Chromoblastomycosis","volume":"30","author":"Santos","year":"2017","journal-title":"Clin. 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