{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T01:28:15Z","timestamp":1793755695339,"version":"4.6.1"},"reference-count":31,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T00:00:00Z","timestamp":1649894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Background: The diagnosis of CPA relies on the detection of the IgG Aspergillus antibody, which is not freely available, especially in resource-poor settings. Point-of-care tests like LDBio Aspergillus ICT lateral flow assay, evaluated in only a few studies, have shown promising results for the diagnosis of CPA. However, no study has compared the diagnostic performances of LDBio LFA in setting of tuberculosis endemic countries and have compared it with that of IgG Aspergillus. Objectives: This study aimed to evaluate the diagnostic performances of LDBio LFA in CPA and compare it with existing the diagnostic algorithm utilising ImmunoCAP IgG Aspergillus. Methods: Serial patients presenting with respiratory symptoms (cough, haemoptysis, fever, etc.) for &gt;4 weeks were screened for eligibility. Relevant investigations, including direct microscopy and culture of respiratory secretions, IgG Aspergillus, chest imaging, etc., were done according to existing algorithm. Serums of all patients were tested by LDBio LFA and IgG Aspergillus (ImmunoCAP Asp IgG) and their diagnostic performances were compared. Results: A total of 174 patients were included in the study with ~66.7% patients having past history of tuberculosis. A diagnosis of CPA was made in 74 (42.5%) of patients. The estimated sensitivity and specificity of LDBio LFA was 67.6% (95% CI: 55.7\u201378%) and 81% (95% CI: 71.9\u201388.2%), respectively, which increased to 73.3% (95% CI: 60.3\u201383.9%) and 83.9% (95% CI: 71.7\u201392.4%), respectively, in patients with a past history of tuberculosis. The sensitivity and specificity of IgG Aspergillus was 82.4% (95% CI: 71.8\u201390.3%) and 82% (95% CI: 73.1\u201389%); 86.7% (95% CI: 75.4\u201394.1%) and 80.4% (95% CI: 67.6\u201389.8%), in the whole group and those with past history of tuberculosis, respectively. Conclusions: LDBio LFA is a point-of-care test with reasonable sensitivity and specificity. However, further tests may have to be done to rule-in or rule-out the diagnosis of CPA in the appropriate setting.<\/jats:p>","DOI":"10.3390\/jof8040400","type":"journal-article","created":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T04:21:28Z","timestamp":1650255688000},"page":"400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Efficacy of LD Bio Aspergillus ICT Lateral Flow Assay for Serodiagnosis of Chronic Pulmonary Aspergillosis"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9434-5338","authenticated-orcid":false,"given":"Animesh","family":"Ray","sequence":"first","affiliation":[{"name":"Department of Medicine, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3213-4065","authenticated-orcid":false,"given":"Mohit","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Medicine, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6283-4575","authenticated-orcid":false,"given":"Janya","family":"Sachdev","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prayas","family":"Sethi","sequence":"additional","affiliation":[{"name":"Department of Medicine, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ved Prakash","family":"Meena","sequence":"additional","affiliation":[{"name":"Department of Medicine, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3169-8419","authenticated-orcid":false,"given":"Gagandeep","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Immaculata","family":"Xess","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4465-9360","authenticated-orcid":false,"given":"Surabhi","family":"Vyas","sequence":"additional","affiliation":[{"name":"Department of Radiodiagnosis, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maroof Ahmad","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Medicine, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-2251","authenticated-orcid":false,"given":"David W.","family":"Denning","sequence":"additional","affiliation":[{"name":"Manchester Fungal Infection Group, Manchester Academic Health Science Centre, University of Manchester, Manchester M13 9NT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveet","family":"Wig","sequence":"additional","affiliation":[{"name":"Department of Medicine, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2616-9157","authenticated-orcid":false,"given":"Sushil Kumar","family":"Kabra","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, AIIMS, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1136\/thoraxjnl-2014-206291","article-title":"The clinical spectrum of pulmonary aspergillosis","volume":"70","author":"Kosmidis","year":"2015","journal-title":"Thorax"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/myc.13369","article-title":"COVID-19-associated subacute invasive pulmonary aspergillosis","volume":"65","author":"Swain","year":"2022","journal-title":"Mycoses"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/myc.12152","article-title":"Chronic pulmonary aspergillosis","volume":"57","author":"Schweer","year":"2014","journal-title":"Mycoses"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1183\/13993003.00583-2015","article-title":"Chronic pulmonary aspergillosis: Rationale and clinical guidelines for diagnosis and management","volume":"47","author":"Denning","year":"2016","journal-title":"Eur. 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