{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T11:01:49Z","timestamp":1783854109099,"version":"3.55.0"},"reference-count":56,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T00:00:00Z","timestamp":1651276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation (FAPESP)","doi-asserted-by":"publisher","award":["2018\/02513-9"],"award-info":[{"award-number":["2018\/02513-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation (FAPESP)","doi-asserted-by":"publisher","award":["88887.468793\/2019-00"],"award-info":[{"award-number":["88887.468793\/2019-00"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Capes-PrInt-Unesp","award":["2018\/02513-9"],"award-info":[{"award-number":["2018\/02513-9"]}]},{"name":"Capes-PrInt-Unesp","award":["88887.468793\/2019-00"],"award-info":[{"award-number":["88887.468793\/2019-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Denture stomatitis (DS) is a common infection in denture wearers, especially women. This study evaluated the induction of DS using acrylic devices attached to the palate of rats combined with inoculation of Candida spp. Immunocompetent male and female rats received a carbohydrate-rich diet. Impressions were taken from the rats\u2019 palate to individually fabricate acrylic devices. Mono- and multispecies biofilms of C. albicans, C. glabrata, and C. tropicalis were grown on the devices, which were then cemented on posterior teeth and kept in the rats\u2019 palate for four weeks. Microbial samples from the palate and the device were quantified. Oral microbiome of rats inoculated with C. albicans was analyzed by 16S rRNA gene sequencing. Log10(CFU\/mL) were analyzed by mixed or two-way MANOVA (\u03b1 = 0.05). Candida spp. and acrylic device did not induce palatal inflammation macroscopically nor microscopically. Although there was an increase (p &lt; 0.001) of the total microbiota and female rats demonstrated higher (p = 0.007) recovery of Candida spp. from the palate, the gender differences were not biologically relevant. The microbiome results indicate an increase in inflammatory microbiota and reduction in health-associated micro-organisms. Although Candida spp. and acrylic device did not induce DS in immunocompetent rats, the shift in microbiota may precede manifestation of inflammation.<\/jats:p>","DOI":"10.3390\/jof8050466","type":"journal-article","created":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T05:44:07Z","timestamp":1651297447000},"page":"466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats"],"prefix":"10.3390","volume":"8","author":[{"given":"Vinicius Tatsuyuji","family":"Sakima","sequence":"first","affiliation":[{"name":"Laboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, Araraquara, S\u00e3o Paulo State University (UNESP), S\u00e3o Paulo 14801-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9370-4151","authenticated-orcid":false,"given":"Yuliana","family":"Vega-Chac\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, Araraquara, S\u00e3o Paulo State University (UNESP), S\u00e3o Paulo 14801-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5756-5828","authenticated-orcid":false,"given":"Paulo Sergio","family":"Cerri","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Embryology, Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, School of Dentistry, Araraquara, S\u00e3o Paulo State University (UNESP), S\u00e3o Paulo 14801-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5477-1922","authenticated-orcid":false,"given":"Bhumika","family":"Shokeen","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of California at Los Angeles (UCLA), Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Lux","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of California at Los Angeles (UCLA), Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9575-7625","authenticated-orcid":false,"given":"Ewerton Garcia de Oliveira","family":"Mima","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, Araraquara, S\u00e3o Paulo State University (UNESP), S\u00e3o Paulo 14801-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.cden.2016.12.004","article-title":"Oral fungal infections: Diagnosis and management","volume":"61","author":"Telles","year":"2017","journal-title":"Dent. 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