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While the antifungals available are defied by biofilm formation and resistance emergence, antimicrobial photodynamic inactivation (aPDI) arises as an alternative antifungal therapy. The tetracationic metalloporphyrin Zn(II) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin (ZnTnHex-2-PyP4+) has high photoefficiency and improved cellular interactions. We investigated the ZnTnHex-2-PyP4+ as a photosensitizer (PS) to photoinactivate yeasts and biofilms of C. albicans strains (ATCC 10231 and ATCC 90028) using a blue light-emitting diode. The photoinactivation of yeasts was evaluated by quantifying the colony forming units. The aPDI of ATCC 90028 biofilms was assessed by the MTT assay, propidium iodide (PI) labeling, and scanning electron microscopy. Mammalian cytotoxicity was investigated in Vero cells using MTT assay. The aPDI (4.3 J\/cm2) promoted eradication of yeasts at 0.8 and 1.5 \u00b5M of PS for ATCC 10231 and ATCC 90028, respectively. At 0.8 \u00b5M and same light dose, aPDI-treated biofilms showed intense PI labeling, about 89% decrease in the cell viability, and structural alterations with reduced hyphae. No considerable toxicity was observed in mammalian cells. Our results introduce the ZnTnHex-2-PyP4+ as a promising PS to photoinactivate both yeasts and biofilms of C. albicans, stimulating studies with other Candida species and resistant isolates.<\/jats:p>","DOI":"10.3390\/jof8060556","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:14:14Z","timestamp":1653437654000},"page":"556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Photoinactivation of Yeast and Biofilm Communities of Candida albicans Mediated by ZnTnHex-2-PyP4+ Porphyrin"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3224-6884","authenticated-orcid":false,"given":"Sueden O.","family":"Souza","sequence":"first","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno L.","family":"Raposo","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2330-5494","authenticated-orcid":false,"given":"Jos\u00e9 F.","family":"Sarmento-Neto","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Universidade Federal da Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3818-029X","authenticated-orcid":false,"given":"J\u00falio S.","family":"Rebou\u00e7as","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Universidade Federal da Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5305-5545","authenticated-orcid":false,"given":"Danielle P. C.","family":"Mac\u00eado","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Pernambuco, Recife 50740-520, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina C. B. Q.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto Aggeu Magalh\u00e3es\u2014Funda\u00e7\u00e3o Oswaldo Cruz (IAM-FIOCRUZ), Recife 50740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9909-5462","authenticated-orcid":false,"given":"Beate S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Pernambuco, Recife 50740-520, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5018-9126","authenticated-orcid":false,"given":"Anderson Z.","family":"Freitas","sequence":"additional","affiliation":[{"name":"Centro de Lasers e Aplica\u00e7\u00f5es, Instituto de Pesquisas Energ\u00e9ticas e Nucleares (IPEN-CNEN), S\u00e3o Paulo 05508-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1628-7769","authenticated-orcid":false,"given":"Paulo E.","family":"Cabral Filho","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4203-1134","authenticated-orcid":false,"given":"Martha S.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Centro de Lasers e Aplica\u00e7\u00f5es, Instituto de Pesquisas Energ\u00e9ticas e Nucleares (IPEN-CNEN), S\u00e3o Paulo 05508-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4675-2163","authenticated-orcid":false,"given":"Adriana","family":"Fontes","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1439-0507.2008.01606.x","article-title":"Epidemiological Trends in Skin Mycoses Worldwide","volume":"51","author":"Havlickova","year":"2008","journal-title":"Mycoses"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Bongomin, F., Gago, S., Oladele, R.O., and Denning, D.W. 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