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Recent studies based on omics suggest that marine fungi potentially play a major role in elemental cycles. However, there is very limited information on the diversity of extracellular enzymatic activities performed by pelagic fungi in the ocean and how these might be affected by community composition and\/or critical environmental parameters such as temperature. In order to obtain information on the potential metabolic activity of marine fungi, extracellular enzymatic activities (EEA) were investigated. Five marine fungal species belonging to the most abundant pelagic phyla (Ascomycota and Basidiomycota) were grown at 5 \u00b0C and 20 \u00b0C, and fluorogenic enzymatic assays were performed using six substrate analogues for the hydrolysis of carbohydrates (\u03b2-glucosidase, \u03b2-xylosidase, and N-acetyl-\u03b2-D-glucosaminidase), amino acids (leucine aminopeptidase), and of organic phosphorus (alkaline phosphatase) and sulfur compounds (sulfatase). Remarkably, all fungal strains were capable of hydrolyzing all the offered substrates. However, the hydrolysis rate (Vmax) and half-saturation constant (Km) varied among the fungal strains depending on the enzyme type. Temperature had a strong impact on the EEAs, resulting in Q10 values of up to 6.1 and was species and substrate dependent. The observed impact of temperature on fungal EEA suggests that warming of the global ocean might alter the contribution of pelagic fungi in marine biogeochemical cycles.<\/jats:p>","DOI":"10.3390\/jof8060571","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T07:05:07Z","timestamp":1653635107000},"page":"571","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Extracellular Enzymatic Activities of Oceanic Pelagic Fungal Strains and the Influence of Temperature"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-4489","authenticated-orcid":false,"given":"Katherine","family":"Salazar Alekseyeva","sequence":"first","affiliation":[{"name":"Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard J.","family":"Herndl","sequence":"additional","affiliation":[{"name":"Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, Austria"},{"name":"Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research (NIOZ), University of Utrecht, 1790 Texel, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8907-1494","authenticated-orcid":false,"given":"Federico","family":"Baltar","sequence":"additional","affiliation":[{"name":"Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-62703-712-9_1","article-title":"Methods for isolation and cultivation of filamentous fungi","volume":"1096","author":"Nevalainen","year":"2014","journal-title":"Methods Mol. 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