{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T20:05:42Z","timestamp":1778011542267,"version":"3.51.4"},"reference-count":22,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004606","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG8L1001"],"award-info":[{"award-number":["CMRPG8L1001"]}],"id":[{"id":"10.13039\/501100004606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The risk of developing coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) depends on factors related to the host, virus, and treatment. However, many hospitals have modified their existing rooms and adjusted airflow to protect healthcare workers from aerosolization, which may increase the risk of Aspergillus exposure. This study aimed to quantitatively investigate airborne fungal levels in negative and slightly negative pressure rooms for COVID-19 patients. The air in neutral pressure rooms in ordinary wards and a liver intensive care unit with high-efficiency particulate air filter was also assessed for comparison. We found the highest airborne fungal burden in recently renovated slightly negative air pressure rooms, and a higher airborne fungal concentration in both areas used to treat COVID-19 patients. The result provided evidence of the potential environmental risk of CAPA by quantitative microbiologic air sampling, which was scarcely addressed in the literature. Enhancing environmental infection control measures to minimize exposure to fungal spores should be considered. However, the clinical implications of a periodic basis to determine indoor airborne fungal levels and further air sterilization in these areas remain to be defined.<\/jats:p>","DOI":"10.3390\/jof8070692","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:21:50Z","timestamp":1656634910000},"page":"692","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Air Sampling for Fungus around Hospitalized Patients with Coronavirus Disease 2019"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5192-6821","authenticated-orcid":false,"given":"Yi-Chun","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin-Shiou","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1021-0429","authenticated-orcid":false,"given":"Shu-Fang","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan"},{"name":"Department of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9302-5220","authenticated-orcid":false,"given":"Chen-Hsiang","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan"},{"name":"School of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Arastehfar, A., Carvalho, A., van de Veerdonk, F.L., Jenks, J.D., Koehler, P., Krause, R., Cornely, O.A., Perlin, D.S., Lass-Florl, C., and Hoenigl, M. (2020). COVID-19 Associated Pulmonary Aspergillosis (CAPA)-From Immunology to Treatment. J. Fungi, 6.","DOI":"10.3390\/jof6020091"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e149","DOI":"10.1016\/S1473-3099(20)30847-1","article-title":"Defining and managing COVID-19-associated pulmonary aspergillosis: The 2020 ECMM\/ISHAM consensus criteria for research and clinical guidance","volume":"21","author":"Koehler","year":"2021","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.3201\/eid2704.204895","article-title":"COVID-19-Associated Pulmonary Aspergillosis, March-August 2020","volume":"27","author":"Sprute","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e3606","DOI":"10.1093\/cid\/ciaa1065","article-title":"Epidemiology of Invasive Pulmonary Aspergillosis Among Intubated Patients With COVID-19: A Prospective Study","volume":"73","author":"Bartoletti","year":"2021","journal-title":"Clin. Infect Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"ofab510","DOI":"10.1093\/ofid\/ofab510","article-title":"Coronavirus Disease 2019-Associated Invasive Fungal Infection","volume":"8","author":"Baddley","year":"2021","journal-title":"Open Forum Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.52586\/5053","article-title":"Potential links between COVID-19-associated pulmonary aspergillosis and bronchiectasis as detected by high resolution computed tomography","volume":"26","author":"Baratella","year":"2021","journal-title":"Front. Biosci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Pelosi, P., Tonelli, R., Torregiani, C., Baratella, E., Confalonieri, M., Battaglini, D., Marchioni, A., Confalonieri, P., Clini, E., and Salton, F. (2022). Different Methods to Improve the Monitoring of Noninvasive Respiratory Support of Patients with Severe Pneumonia\/ARDS Due to COVID-19: An Update. J. Clin. Med., 11.","DOI":"10.3390\/jcm11061704"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e1634","DOI":"10.1093\/cid\/ciaa1298","article-title":"A National Strategy to Diagnose Coronavirus Disease 2019-Associated Invasive Fungal Disease in the Intensive Care Unit","volume":"73","author":"White","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S2213-2600(21)00442-2","article-title":"Fungal infections in mechanically ventilated patients with COVID-19 during the first wave: The French multicentre MYCOVID study","volume":"10","author":"Gangneux","year":"2022","journal-title":"Lancet Respir. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1056\/NEJMra2027424","article-title":"Aspergillus Infections","volume":"385","author":"Thompson","year":"2021","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1186\/s13054-020-03221-w","article-title":"Impact of negative air pressure in ICU rooms on the risk of pulmonary aspergillosis in COVID-19 patients","volume":"24","author":"Ichai","year":"2020","journal-title":"Crit. Care"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.jmii.2017.08.017","article-title":"Effectiveness of an ultraviolet-C disinfection system for reduction of healthcare-associated pathogens","volume":"52","author":"Yang","year":"2019","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"248","DOI":"10.3109\/1040841X.2013.777687","article-title":"Indoor fungal contamination: Health risks and measurement methods in hospitals, homes and workplaces","volume":"40","author":"Meheust","year":"2014","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"205","DOI":"10.4236\/aim.2015.54020","article-title":"Identification key for Aspergillus species isolated from maize and soil of Nandi County, Kenya","volume":"5","author":"Nyongesa","year":"2015","journal-title":"Adv. Microbiol."},{"key":"ref_15","unstructured":"Bennett, J.E., Dolin, R., and Blaser, M.J. (2019). Asperfillus Speices. Mandell, Douglas, and Bennett\u2019s Principles and Practice of Infectious Diseases E-Book, Elsevier Health Sciences."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1093\/cid\/civ297","article-title":"Review of fungal outbreaks and infection prevention in healthcare settings during construction and renovation","volume":"61","author":"Kanamori","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.jhin.2006.02.014","article-title":"Nosocomial aspergillosis in outbreak settings","volume":"63","author":"Vonberg","year":"2006","journal-title":"J. Hosp. Infect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.idc.2021.08.002","article-title":"Nosocomial Fungal Infections: Epidemiology, Infection Control, and Prevention","volume":"35","author":"Suleyman","year":"2021","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S2213-2600(21)00138-7","article-title":"COVID-19-associated Aspergillus tracheobronchitis: The interplay between viral tropism, host defence, and fungal invasion","volume":"9","author":"Bruggemann","year":"2021","journal-title":"Lancet Respir. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1053\/jhin.2001.0998","article-title":"Relationship between environmental fungal contamination and the incidence of invasive aspergillosis in haematology patients","volume":"48","author":"Alberti","year":"2001","journal-title":"J. Hosp. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.3389\/fmicb.2020.01671","article-title":"Metagenomic Characterization of Indoor Dust Bacterial and Fungal Microbiota in Homes of Asthma and Non-asthma Patients Using Next Generation Sequencing","volume":"11","author":"Gangneux","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.puhe.2019.07.012","article-title":"Demolition activities in a healthcare facility: Results from a fungal surveillance after extraordinary preventive measures","volume":"175","author":"Troiano","year":"2019","journal-title":"Public Health"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/7\/692\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:40:48Z","timestamp":1760139648000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/7\/692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,30]]},"references-count":22,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["jof8070692"],"URL":"https:\/\/doi.org\/10.3390\/jof8070692","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,30]]}}}