{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T12:09:50Z","timestamp":1776082190070,"version":"3.50.1"},"reference-count":26,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Coccidioidomycosis is a disease caused by the dimorphic fungi Coccidioides spp., which affects humans and a variety of animal species, including domestic dogs. In dogs, accurate diagnosis could provide a substantial improvement on the quality of canine life, as well as an advancement in the mapping of regions endemic for coccidioidomycosis. The purpose of this study was to compare immunodiagnostic assays for anti-Coccidioides antibody (Ab) detection in dogs\u2019 serum. Three commercially available immunodiagnostic assays (IMMY\u00ae; Norman, OK, USA) were evaluated, including the s\u014dna Coccidioides Ab Lateral Flow Assay (LFA), Coccidioides IDCF immunodiffusion assay (IDCF), and the Clarus Coccidioides Ab Enzyme Immunoassay (EIA). Assays were evaluated using 98 dog serum samples: 29 from dogs with coccidioidomycosis, 15 from dogs diagnosed with histoplasmosis, 10 from dogs diagnosed with blastomycosis, and 44 from dogs without a fungal disease. Using specimens from dogs with coccidioidomycosis, the IDCF had an accuracy of 92% (95% confidence interval [95% CI] = 85\u201396%), the EIA had an accuracy of 91% (95% CI = 83\u201396%), and the LFA displayed an accuracy of 82% (95% CI = 73\u201389%). Using Kappa analysis, the agreement between LFA and EIA was 0.59 (95% CI = 0.42\u20130.75), that between LFA and IDCF was 0.64 (95% CI = 0.48\u20130.79), and that between EIA and IDCF was 0.79 (95% CI = 0.64\u20130.90). Most cross-reactions were observed in dogs with histoplasmosis. Compared with EIA and IDCF, the LFA requires substantially less laboratory equipment and infrastructure and rapidly produces results, offering a substantial improvement for the initial screening of coccidioidomycosis in dogs.<\/jats:p>","DOI":"10.3390\/jof8070728","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T22:06:00Z","timestamp":1657749960000},"page":"728","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of Immunodiagnostic Assays for the Rapid Diagnosis of Coccidioidomycosis in Dogs"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"Caceres","sequence":"first","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30333, USA"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, 6525GA Nijmegen, The Netherlands"},{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogot\u00e1 1653, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark D.","family":"Lindsley","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.ss6807a1","article-title":"Surveillance for Coccidioidomycosis\u2014United States, 2011\u20132017","volume":"68","author":"Benedict","year":"2019","journal-title":"MMWR Surveill. Summ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S30","DOI":"10.1093\/mmy\/myy095","article-title":"Update on the Epidemiology of coccidioidomycosis in the United States","volume":"57","author":"McCotter","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.3201\/eid2408.171595","article-title":"Enhanced Surveillance for Coccidioidomycosis, 14 US States, 2016","volume":"24","author":"Benedict","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"417","DOI":"10.3201\/eid2403.170623","article-title":"Coccidioidomycosis Outbreaks, United States and Worldwide, 1940\u20132015","volume":"24","author":"Freedman","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S46","DOI":"10.1093\/mmy\/myy037","article-title":"Coccidioidomycosis in Latin America","volume":"57","author":"Arathoon","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_6","first-page":"785","article-title":"Epidemiology of endemic systemic fungal infections in Latin America","volume":"49","author":"Colombo","year":"2011","journal-title":"Med. Mycol."},{"key":"ref_7","unstructured":"CDC (2022, April 19). Valley Fever (Coccidioidomycosis) Statistics 2020, Available online: https:\/\/www.cdc.gov\/fungal\/diseases\/coccidioidomycosis\/statistics.html."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.3201\/eid1407.070832","article-title":"Testing for coccidioidomycosis among patients with community-acquired pneumonia","volume":"14","author":"Chang","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"958","DOI":"10.3201\/eid1206.060028","article-title":"Coccidioidomycosis as a common cause of community-acquired pneumonia","volume":"12","author":"Valdivia","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.1111\/tbed.13265","article-title":"Soil and climactic predictors of canine coccidioidomycosis seroprevalence in Washington State: An ecological cross-sectional study","volume":"66","author":"Meisner","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.3201\/eid2501.180411","article-title":"Autochthonous Transmission of Coccidioides in Animals, Washington, USA","volume":"25","author":"James","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1111\/tbed.13088","article-title":"Coccidioidomycosis in alpacas in the southwestern United States","volume":"66","author":"Butkiewicz","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1177\/0300985818787306","article-title":"Coccidioidomycosis in Nonhuman Primates: Pathologic and Clinical Findings","volume":"55","author":"Koistinen","year":"2018","journal-title":"Vet. Pathol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1177\/1098612X19829910","article-title":"Clinical features of cats diagnosed with coccidioidomycosis in Arizona, 2004\u20132018","volume":"22","author":"Arbona","year":"2020","journal-title":"J. Feline Med. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"346-e87","DOI":"10.1111\/vde.12356","article-title":"Retrospective analysis of cutaneous lesions in 23 canine and 17 feline cases of coccidioidomycosis seen in Arizona, USA (2009\u20132015)","volume":"27","author":"Simoes","year":"2016","journal-title":"Vet. Dermatol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"226","DOI":"10.5326\/0440226","article-title":"Coccidioidomycosis in dogs and cats: A review","volume":"44","author":"Valentine","year":"2008","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.idc.2015.10.008","article-title":"Coccidioidomycosis","volume":"30","author":"Stockamp","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Hospenthal, D.R., and Rinaldi, M.G. (2015). Diagnostic Immunology. Diagnosis and Treatment of Fungal Infections, Springer International Publishing.","DOI":"10.1007\/978-3-319-13090-3"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Detrick, B., Schmitz, J.L., and Hamilton, R.G. (2016). Serological and Molecular Diagnosis of Fungal Infections. Manual of Molecular and Clinical Laboratory Immunology, ASM. [8th ed.].","DOI":"10.1128\/9781555818722"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chow, N.A., Lindsley, M.D., McCotter, O.Z., Kangiser, D., Wohrle, R.D., Clifford, W.R., Yaglom, H.D., Adams, L.E., Komatsu, K., and Durkin, M.M. (2017). Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0175081"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1093\/mmy\/myy157","article-title":"Novel canine anti-Coccidioides immunoglobulin G enzyme immunoassay aids in diagnosis of coccidioidomycosis in dogs","volume":"57","author":"Holbrook","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_22","unstructured":"IMMY (2022, June 12). Sona Coccidioides Ab LFA 2020. Available online: https:\/\/immy.com\/cta."},{"key":"ref_23","unstructured":"Orozco-Vargas, L.C. (2010). Medicion en Salud: Diagnostico y Evaluacion de Resultados: Un Manual Critico Mas Alla de lo Basic, Universidad Industrial de Santander."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"276","DOI":"10.11613\/BM.2012.031","article-title":"Interrater reliability: The kappa statistic","volume":"22","author":"McHugh","year":"2012","journal-title":"Biochem. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1093\/mmy\/myz067","article-title":"Evaluation of a commercially available, point-of-care Coccidioides antibody lateral flow assay to aid in rapid diagnosis of coccidioidomycosis in dogs","volume":"58","author":"Schlacks","year":"2020","journal-title":"Med. Mycol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1111\/jvim.16087","article-title":"Clinical performance of a point-of-care Coccidioides antibody test in dogs","volume":"35","author":"Reagan","year":"2021","journal-title":"J. Vet. Intern. Med."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/7\/728\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:49:29Z","timestamp":1760140169000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/7\/728"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,13]]},"references-count":26,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["jof8070728"],"URL":"https:\/\/doi.org\/10.3390\/jof8070728","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,13]]}}}