{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T12:46:11Z","timestamp":1775479571947,"version":"3.50.1"},"reference-count":28,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["CNPq 302796\/2017-7"],"award-info":[{"award-number":["CNPq 302796\/2017-7"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["FAPERJ E-26\/202.527\/2019"],"award-info":[{"award-number":["FAPERJ E-26\/202.527\/2019"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","award":["CNPq 302796\/2017-7"],"award-info":[{"award-number":["CNPq 302796\/2017-7"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","award":["FAPERJ E-26\/202.527\/2019"],"award-info":[{"award-number":["FAPERJ E-26\/202.527\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Besides the relevance of aspergillosis in neutropenic patients, this mycosis has gained significance among non-neutropenic patients in last years. The detection of Aspergillus galactomannan has been used for aspergillosis diagnosis and follow-up in neutropenic patients. This study evaluated the applicability of two commercial tests for galactomannan detection in non-neutropenic patients with different clinical forms of aspergillosis. Serum samples from patients with chronic pulmonary aspergillosis, aspergilloma, invasive aspergillosis, and COVID-19 associated pulmonary aspergillosis were evaluated using the IMMY s\u014dna AGM lateral flow assay and the Bio-Rad Platelia sandwich ELISA. Serum specimens from patients with tuberculosis, histoplasmosis, paracoccidioidomycosis, and from healthy individuals were used as controls. The Bio-Rad Platelia sandwich ELISA presented greater sensitivity, whereas the IMMY s\u014dna AGM lateral flow assay presented greater specificity. The accuracies of the tests were similar, as demonstrated by a receiver operator characteristic analysis. Moreover, the best cut-off values determined by this analysis were closer to that recommended by both manufacturers for neutropenic patients. The galactomannan indexes determined by different methodologies were strongly related, and a substantial agreement was observed between results. Both tests can be used in non-neutropenic patients with the cut-off values defined by the manufacturers. Histoplasma cross-reactions may occur in areas where histoplasmosis is endemic.<\/jats:p>","DOI":"10.3390\/jof8070741","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T11:32:23Z","timestamp":1658143943000},"page":"741","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Performance of Two Commercial Assays for the Detection of Serum Aspergillus Galactomannan in Non-Neutropenic Patients"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6639-6058","authenticated-orcid":false,"given":"Rodrigo","family":"Almeida-Paes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5581-631X","authenticated-orcid":false,"given":"Marcos de Abreu","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6900-9703","authenticated-orcid":false,"given":"Priscila Marques","family":"de Macedo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica em Dermatologia Infecciosa, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"Caceres","sequence":"additional","affiliation":[{"name":"Immuno-Mycologics (IMMY), Norman, OK 73069, USA"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, 6525GA Nijmegen, The Netherlands"},{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 1653, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosely Maria","family":"Zancop\u00e9-Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00140-18","DOI":"10.1128\/CMR.00140-18","article-title":"Aspergillus fumigatus and Aspergillosis in 2019","volume":"33","author":"Chamilos","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1097\/QCO.0000000000000381","article-title":"Immune responses to invasive aspergillosis: New understanding and therapeutic opportunities","volume":"30","author":"Hohl","year":"2017","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1055\/s-0039-3401990","article-title":"Invasive Pulmonary Aspergillosis","volume":"41","author":"Ledoux","year":"2020","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Costantini, C., van de Veerdonk, F.L., and Romani, L. (2020). Covid-19-Associated Pulmonary Aspergillosis: The Other Side of the Coin. Vaccines, 8.","DOI":"10.3390\/vaccines8040713"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jhin.2021.04.012","article-title":"Incidence, diagnosis and outcomes of COVID-19-associated pulmonary aspergillosis (CAPA): A systematic review","volume":"113","author":"Chong","year":"2021","journal-title":"J. Hosp. Infect."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/ciw326","article-title":"Practice Guidelines for the Diagnosis and Management of Aspergillosis: 2016 Update by the Infectious Diseases Society of America","volume":"63","author":"Patterson","year":"2016","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1055\/s-0035-1562892","article-title":"Role of Non-Culture-Based Tests, with an Emphasis on Galactomannan Testing for the Diagnosis of Invasive Aspergillosis","volume":"36","author":"Miceli","year":"2015","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1128\/CVI.00310-08","article-title":"Cross-reactivity of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Cryptococcus species in the commercial Platelia Aspergillus enzyme immunoassay","volume":"16","author":"Xavier","year":"2009","journal-title":"Clin. Vaccine Immunol. CVI"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1128\/jcm.33.2.497-500.1995","article-title":"A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis","volume":"33","author":"Stynen","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e00053-20","DOI":"10.1128\/JCM.00053-20","article-title":"Evaluation of the Performance of the IMMY sona Aspergillus Galactomannan Lateral Flow Assay When Testing Serum To Aid in Diagnosis of Invasive Aspergillosis","volume":"58","author":"White","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and Update of the Consensus Definitions of Invasive Fungal Disease From the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium","volume":"71","author":"Donnelly","year":"2020","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e149","DOI":"10.1016\/S1473-3099(20)30847-1","article-title":"Defining and managing COVID-19-associated pulmonary aspergillosis: The 2020 ECMM\/ISHAM consensus criteria for research and clinical guidance","volume":"21","author":"Koehler","year":"2021","journal-title":"Lancet Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"213","DOI":"10.32614\/RJ-2016-042","article-title":"easyROC: An Interactive Web-tool for ROC Curve Analysis Using R Language Environment","volume":"8","author":"Goksuluk","year":"2016","journal-title":"R J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.bbmt.2018.09.028","article-title":"Mainly Post-Transplant Factors Are Associated with Invasive Aspergillosis after Allogeneic Stem Cell Transplantation: A Study from the Surveillance des Aspergilloses Invasives en France and Soci\u00e9t\u00e9 Francophone de Greffe de Moelle et de Th\u00e9rapie Cellulaire","volume":"25","author":"Robin","year":"2019","journal-title":"Biol. Blood Marrow Transplant. J. Am. Soc. Blood Marrow Transplant."},{"key":"ref_15","first-page":"99","article-title":"Diagn\u00f3stico temprano de aspergilosis invasiva en pacientes neutrop\u00e9nicos con detecci\u00f3n seriada quincenal de galactomanano circulante por Platelia Aspergillus","volume":"20","author":"Pazos","year":"2003","journal-title":"Rev. Iberoam. Micol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1080\/14737159.2020.1820864","article-title":"Point-of-care diagnostics for invasive aspergillosis: Nearing the finish line","volume":"20","author":"Jenks","year":"2020","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/myc.12765","article-title":"Diagnostic value of serum galactomannan, (1,3)-\u03b2-d-glucan, and Aspergillus fumigatus-specific IgA and IgG assays for invasive pulmonary aspergillosis in non-neutropenic patients","volume":"61","author":"Dobias","year":"2018","journal-title":"Mycoses"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1128\/JCM.00345-17","article-title":"Diagnostic Value of Galactomannan Antigen Test in Serum and Bronchoalveolar Lavage Fluid Samples from Patients with Nonneutropenic Invasive Pulmonary Aspergillosis","volume":"55","author":"Zhou","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1007\/s10096-019-03469-3","article-title":"Clinical features and cause analysis of false positive results of Aspergillus galactomannan assay in pulmonary cryptococcosis patients","volume":"38","author":"Takazono","year":"2019","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1128\/JCM.05946-11","article-title":"Cross-reactivity of Fusarium spp. in the Aspergillus Galactomannan enzyme-linked immunosorbent assay","volume":"50","author":"Tortorano","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3109\/13693786.2011.608172","article-title":"Cross-reactivity in the PlateliaTM Aspergillus enzyme immunoassay caused by blastomycosis","volume":"50","author":"Lewis","year":"2012","journal-title":"Med. Mycol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s10096-007-0380-7","article-title":"Platelia Aspergillus assay for diagnosis of disseminated histoplasmosis","volume":"26","author":"Ranque","year":"2007","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/j.ijmm.2014.07.016","article-title":"A complementary tool for management of disseminated Histoplasma capsulatum var. capsulatum infections in AIDS patients","volume":"304","author":"Iriart","year":"2014","journal-title":"Int. J. Med. Microbiol. IJMM"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"de Macedo, P.M., Freitas, A.D., B\u00e1rtholo, T.P., Bernardes-Engemann, A.R., de Almeida, M.A., Almeida-Silva, F., Zancop\u00e9-Oliveira, R.M., and Almeida-Paes, R. (2021). Acute Pulmonary Histoplasmosis Following COVID-19: Novel Laboratorial Methods Aiding Diagnosis. J. Fungi, 7.","DOI":"10.3390\/jof7050346"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1399-3062.2011.00675.x","article-title":"False-positive Aspergillus galactomannan assay in solid organ transplant recipients with histoplasmosis","volume":"14","author":"Vergidis","year":"2012","journal-title":"Transpl. Infect. Dis. Off. J. Transplant. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"104018","DOI":"10.1016\/j.micpath.2020.104018","article-title":"Aspergillus coinfection among patients with pulmonary tuberculosis in Asia and Africa countries; A systematic review and meta-analysis of cross-sectional studies","volume":"141","author":"Hosseini","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e138","DOI":"10.2741\/944","article-title":"Allergic bronchopulmonary and sinus aspergillosis: The roentgenologic spectrum","volume":"8","author":"Shah","year":"2003","journal-title":"Front. Biosci. J. Virtual Libr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1097\/00005792-198203000-00005","article-title":"Chronic necrotizing pulmonary aspergillosis: A discrete clinical entity","volume":"61","author":"Binder","year":"1982","journal-title":"Medicine"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/7\/741\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:53:19Z","timestamp":1760140399000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/7\/741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,18]]},"references-count":28,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["jof8070741"],"URL":"https:\/\/doi.org\/10.3390\/jof8070741","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,18]]}}}