{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:57:06Z","timestamp":1776301026969,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French National Research Agency","award":["20CE35000701"],"award-info":[{"award-number":["20CE35000701"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Standardized, reproducible and validated Mucorales quantitative PCR (qPCR) assays are needed in the context of routine testing in diagnostic labs. We, therefore, compared the commercial MucorGenius\u00ae assay (PathoNostics, Maastricht) targeting five genera of Mucorales to our in-house qPCR targeting Rhizomucor spp., Lichtheimia spp. and Mucor\/Rhizopus spp. To assess their analytical sensitivity, 25 frozen leftover serum specimens, which had already tested positive based on our in-house assay, were selected. These sera were from 15 patients with probable or proven mucormycosis. For analytical specificity, 0.5 pg from 15 purified fungal DNAs from nine different Mucorales genera were spiked into pooled qPCR-negative leftover serum specimens. All samples were tested in parallel with both assays and the quantitative cycles (Cq) were compared. A total of 13\/25 (52%) serum samples were amplified by one of the two assays with only four of them detected with the MucorGenius\u00ae assay. In spiked specimens, all targeted strains were successfully amplified by our in-house qPCR. The MucorGenius\u00ae assay was not able to detect Lichtheimia corymbifera but successfully amplified all other species targeted by the kit and two additional non-targeted species (Syncephalastrum monosporum and Saksenaea vasiformis). The MucorGenius\u00ae assay showed lower analytical sensitivity compared to our in-house assay. Indeed, the MucorGenius\u00ae assay amplified more species, as expected, but showed a decreased detection of the frequent species Lichtheimia corymbifera.<\/jats:p>","DOI":"10.3390\/jof8080786","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T03:21:16Z","timestamp":1658978476000},"page":"786","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Analytical Performance of the Commercial MucorGenius\u00ae Assay as Compared to an In-House qPCR Assay to Detect Mucorales DNA in Serum Specimens"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7784-4434","authenticated-orcid":false,"given":"Th\u00e9o","family":"Ghelfenstein-Ferreira","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Saint-Louis-Lariboisi\u00e8re-Fernand-Widal, 75010 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Verdurme","sequence":"additional","affiliation":[{"name":"Laboratoire Cerba, Saint-Ouen-l\u2019Aum\u00f4ne, 95310 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9726-3082","authenticated-orcid":false,"given":"Alexandre","family":"Alanio","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Saint-Louis-Lariboisi\u00e8re-Fernand-Widal, 75010 Paris, France"},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Centre National de la Recherche Scientifique (CNRS), Molecular Mycology Unit, Unit\u00e9 Mixte de Recherche UMR2000, CEDEX 15, 75724 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"ref_1","unstructured":"Garcia-Hermoso, D., Alanio, A., Lortholary, O., and Dromer, F. 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Infect."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/8\/786\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:57:32Z","timestamp":1760140652000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/8\/786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,27]]},"references-count":22,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["jof8080786"],"URL":"https:\/\/doi.org\/10.3390\/jof8080786","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,27]]}}}