{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:59:55Z","timestamp":1765486795341,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T00:00:00Z","timestamp":1658966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Ciencia y Tecnolog\u00eda de la Provincia de C\u00f3rdoba, Argentina","award":["PICT 2015-N\u00b01425","PICT 2018-N\u00b01349","CONICET, PIP N\u00b0 11220150100260"],"award-info":[{"award-number":["PICT 2015-N\u00b01425","PICT 2018-N\u00b01349","CONICET, PIP N\u00b0 11220150100260"]}]},{"name":"Secretar\u00eda de Ciencia y Tecnolog\u00eda, Universidad Nacional de C\u00f3rdoba","award":["PICT 2015-N\u00b01425","PICT 2018-N\u00b01349","CONICET, PIP N\u00b0 11220150100260"],"award-info":[{"award-number":["PICT 2015-N\u00b01425","PICT 2018-N\u00b01349","CONICET, PIP N\u00b0 11220150100260"]}]},{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT 2015-N\u00b01425","PICT 2018-N\u00b01349","CONICET, PIP N\u00b0 11220150100260"],"award-info":[{"award-number":["PICT 2015-N\u00b01425","PICT 2018-N\u00b01349","CONICET, PIP N\u00b0 11220150100260"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Lung dendritic cells (DC) are powerful antigen-presenting cells constituted by various subpopulations that differ in terms of their function and origin and differentially regulate cell-mediated antifungal immunity. The lung is the primary target organ of Cryptococcus neoformans and C. gattii infections, which makes it essential in the establishment of the first line of anti-cryptococcal defense. However, the lung-specific dynamics and function of DC subsets are poorly understood in cryptococcosis. In this study, we provide evidence for the in vivo function of a conventional langerin-expressing DC1 dendritic cell (LangDC1) population during the first week of intratracheal C. neoformans infection in mice. By using conditional depletion of LangDC1 after diphtheria toxin treatment of LangDTREGFP mice, we demonstrate that these animals better control the fungal infection and produce type 1 and 17 cytokines in the context of a type 2 immune response, favoring a predominance of iNOS over arginase-1 expression by pulmonary cells. Our results suggest that LangDC1 cells play a role in impairing immune response for the clearance of C. neoformans in the early stage of pulmonary infection.<\/jats:p>","DOI":"10.3390\/jof8080792","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T22:43:26Z","timestamp":1659048206000},"page":"792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice"],"prefix":"10.3390","volume":"8","author":[{"given":"Lorena","family":"Guasconi","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio","family":"Beccacece","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ximena","family":"Volpini","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Instituto de Investigaci\u00f3n M\u00e9dica Mercedes y Mart\u00edn Ferreyra (INIMEC), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5016GCA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica L.","family":"Burstein","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian J.","family":"Mena","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Silvane","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariel A.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melina Mara","family":"Musri","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n M\u00e9dica Mercedes y Mart\u00edn Ferreyra (INIMEC), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5016GCA, Argentina"},{"name":"Departamento de Fisiolog\u00eda, Facultad de Ciencias Exactas, F\u00edsicas y Naturales (FCEFyN), Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5016GCA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Cervi","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura S.","family":"Chiapello","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e170554","DOI":"10.1590\/0074-02760170554","article-title":"The Status of Cryptococcosis in Latin 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Dis."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/8\/792\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:58:43Z","timestamp":1760140723000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/8\/792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,28]]},"references-count":39,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["jof8080792"],"URL":"https:\/\/doi.org\/10.3390\/jof8080792","relation":{},"ISSN":["2309-608X"],"issn-type":[{"type":"electronic","value":"2309-608X"}],"subject":[],"published":{"date-parts":[[2022,7,28]]}}}