{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:50:30Z","timestamp":1790740230334,"version":"4.1.0"},"reference-count":20,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The T2Bacteria panel is a direct-from-blood assay that delivers rapid results, targeting E. coli, S. aureus, K. pneumoniae, A. baumanii, P. aeruginosa, and E. faecium (ESKAPE pathogens). In this study, T2Bacteria and T2Candida (targeting C. albicans\/C. tropicalis, C. glabrata\/C. krusei, and C. parapsilosis) were evaluated in parallel with blood cultures in 101 consecutive surgical patients with suspected intra-abdominal infection admitted to the intensive care unit or high dependency unit. Fifteen patients had bacteremia, with T2Bacteria correctly identifying all on-panel (n = 8) pathogens. T2Bacteria was positive in 19 additional patients, 11 of whom had supportive cultures from other normally sterile sites (newly inserted drains, perioperative cultures or blood cultures) within seven days. Six of these eleven patients (55%) received broad-spectrum antibiotics at the sampling time. T2Candida identified the two cases of blood-culture-positive candidemia and was positive in seven additional patients, three of whom were confirmed to have intra-abdominal candidiasis. Of four patients with concurrent T2Bacteria and T2Candida positivity, only one patient had positive blood cultures (candidemia), while three out of four patients had supporting microbiological evidence of a mixed infection. T2Bacteria and T2Candida were fast and accurate in diagnosing on-panel bloodstream infections, and T2Bacteria was able to detect culture-negative intra-abdominal infections.<\/jats:p>","DOI":"10.3390\/jof8080832","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T02:42:53Z","timestamp":1660099373000},"page":"832","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Combining T2Bacteria and T2Candida Panels for Diagnosing Intra-Abdominal Infections: A Prospective Multicenter Study"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1230-0492","authenticated-orcid":false,"given":"Anders","family":"Krifors","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology, Karolinska Institutet, 14186 Stockholm, Sweden"},{"name":"Centre for Clinical Research V\u00e4stmanland, Uppsala University, 75236 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e5ns","family":"Ullberg","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Karolinska University Hospital, 14186 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Castegren","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, Karolinska Institutet, 14186 Stockholm, Sweden"},{"name":"Perioperative Medicine and Intensive Care (PMI), Karolinska University Hospital, 14186 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Petersson","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, Karolinska Institutet, 14186 Stockholm, Sweden"},{"name":"Perioperative Medicine and Intensive Care (PMI), Karolinska University Hospital, 14186 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Sparrelid","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 14186 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volkan","family":"\u00d6zenci","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Karolinska University Hospital, 14186 Stockholm, Sweden"},{"name":"Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institutet, 14186 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7167-7882","authenticated-orcid":false,"given":"Ola","family":"Blennow","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Karolinska University Hospital, 14186 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"ofy313","DOI":"10.1093\/ofid\/ofy313","article-title":"Sepsis in Intensive Care Unit Patients: Worldwide Data from the Intensive Care over Nations Audit","volume":"5","author":"Sakr","year":"2018","journal-title":"Open Forum Infect. 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Dis."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Metzgar, D., Frinder, M.W., Rothman, R.E., Peterson, S., Carroll, K.C., Zhang, S.X., Avornu, G.D., Rounds, M.A., Carolan, H.E., and Toleno, D.M. (2016). The IRIDICA BAC BSI Assay: Rapid, Sensitive and Culture-Independent Identification of Bacteria and Candida in Blood. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0158186"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"845","DOI":"10.7326\/M18-2772","article-title":"Performance of the T2Bacteria Panel for Diagnosing Bloodstream Infections: A Diagnostic Accuracy Study","volume":"170","author":"Nguyen","year":"2019","journal-title":"Ann. Intern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"ofz503","DOI":"10.1093\/ofid\/ofz503","article-title":"ESKAPE Pathogens in Bloodstream Infections Are Associated with Higher Cost and Mortality but Can Be Predicted Using Diagnoses Upon Admission","volume":"6","author":"Lowery","year":"2019","journal-title":"Open Forum Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1186\/s12879-020-05049-9","article-title":"In-depth analysis of T2Bacteria positive results in patients with concurrent negative blood culture: A case series","volume":"20","author":"Kalligeros","year":"2020","journal-title":"BMC Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Krifors, A., Ullberg, M., Castegren, M., Petersson, J., Sparrelid, E., Hammarstr\u00f6m, H., Sj\u00f6lin, J., \u00d6zenci, V., and Blennow, O. (2022). T2Candida Assay in the Diagnosis of Intraabdominal Candidiasis: A Prospective Multicenter Study. J. Fungi, 8.","DOI":"10.3390\/jof8010086"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"De Waele, J., Lipman, J., Sakr, Y., Marshall, J.C., Vanhems, P., Barrera Groba, C., Leone, M., and Vincent, J.L. (2014). Abdominal infec-tions in the intensive care unit: Characteristics, treatment and determinants of outcome. BMC Infect Dis., 14.","DOI":"10.1186\/1471-2334-14-420"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Silva-Nunes, J., and Cardoso, T. (2019). Intra-abdominal infections: The role of different classifications on the selection of the best antibiotic treatment. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-4604-0"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e1210","DOI":"10.1002\/mbo3.1210","article-title":"Direct detection of ESKAPEc pathogens from whole blood using the T2Bacteria Panel allows early antimicrobial stewardship intervention in patients with sepsis","volume":"10","author":"Drevinek","year":"2021","journal-title":"MicrobiologyOpen"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"iv20","DOI":"10.1093\/jac\/dky049","article-title":"T2Bacteria magnetic resonance assay for the rapid detection of ESKAPEc pathogens directly in whole blood","volume":"73","author":"Posteraro","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"182ra54","DOI":"10.1126\/scitranslmed.3005377","article-title":"T2 Magnetic Resonance Enables Nanoparticle-Mediated Rapid Detection of Candidemia in Whole Blood","volume":"5","author":"Neely","year":"2013","journal-title":"Sci. Transl. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"539","DOI":"10.3389\/fmicb.2019.00539","article-title":"Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review","volume":"10","author":"Mulani","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_19","unstructured":"CDC (2019). Antibiotic Resistance Threats in the United States, 2019. Services DoHaH, CDC."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1469-0691.2012.03906.x","article-title":"Risk factors for and clinical implications of mixed Candida\/bacterial bloodstream infections","volume":"19","author":"Kim","year":"2013","journal-title":"Clin. Microbiol. 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