{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:17:17Z","timestamp":1782742637741,"version":"3.54.5"},"reference-count":33,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T00:00:00Z","timestamp":1660780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Aspergillosis, which is mainly sustained by Aspergillus fumigatus, includes a broad spectrum of diseases. They are usually severe in patients with co-morbidities. The first-line therapy includes triazoles, for which an increasing incidence of drug resistance has been lately described. As a consequence of this, the need for new and alternative antifungal molecules is absolutely necessary. As peptides represent promising antimicrobial molecules, two lipopeptides (C14-NleRR-NH2, C14-WRR-NH2) were tested to assess the antifungal activity against azole-resistant A. fumigatus. Antifungal activity was evaluated by determination of minimum inhibitory concentrations (MICs), time\u2013kill curves, XTT assay, optical microscopy, and checkerboard combination with isavuconazole. Both lipopeptides showed antifungal activity, with MICs ranging from 8 mg\/L to 16 mg\/L, and a dose-dependent effect was confirmed by both time\u2013kill curves and XTT assays. Microscopy showed that hyphae growth was hampered at concentrations equal to or higher than MICs. The rising antifungal resistance highlights the usefulness of novel compounds to treat severe fungal infections. Although further studies assessing the activity of lipopeptides are necessary, these molecules could be effective antifungal alternatives that overcome the current resistances.<\/jats:p>","DOI":"10.3390\/jof8080872","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T23:28:41Z","timestamp":1660865321000},"page":"872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus"],"prefix":"10.3390","volume":"8","author":[{"given":"Simona","family":"Fioriti","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Cirioni","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"},{"name":"Infectious Disease Clinic, Azienda Ospedaliero Universitaria \u201cOspedali Riuniti\u201d, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriana","family":"Simonetti","sequence":"additional","affiliation":[{"name":"Dermatological Unit, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Franca","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"},{"name":"Infectious Diseases Unit, Azienda Ospedaliera Ospedali Riuniti Marche Nord, 61122 Pesaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca","family":"Candelaresi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"},{"name":"Infectious Disease Clinic, Azienda Ospedaliero Universitaria \u201cOspedali Riuniti\u201d, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Pallotta","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"},{"name":"Infectious Disease Clinic, Azienda Ospedaliero Universitaria \u201cOspedali Riuniti\u201d, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3230-6878","authenticated-orcid":false,"given":"Damian","family":"Neubauer","sequence":"additional","affiliation":[{"name":"Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gda\u0144sk, 80-210 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9741-4562","authenticated-orcid":false,"given":"Elzbieta","family":"Kamysz","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry of Biological Macromolecules, Department of Molecular Biotechnology, Faculty of Chemistry, University of Gda\u0144sk, 80-309 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Kamysz","sequence":"additional","affiliation":[{"name":"Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gda\u0144sk, 80-210 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Canovari","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Azienda Ospedaliera Ospedali Riuniti Marche Nord, 61122 Pesaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Brescini","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"},{"name":"Infectious Disease Clinic, Azienda Ospedaliero Universitaria \u201cOspedali Riuniti\u201d, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0772-0214","authenticated-orcid":false,"given":"Gianluca","family":"Morroni","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Barchiesi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy"},{"name":"Infectious Diseases Unit, Azienda Ospedaliera Ospedali Riuniti Marche Nord, 61122 Pesaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.idc.2021.03.008","article-title":"Aspergillosis: Epidemiology, Diagnosis, and Treatment","volume":"35","author":"Cadena","year":"2021","journal-title":"Infect. 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