{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:25:47Z","timestamp":1791725147021,"version":"4.3.4"},"reference-count":26,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T00:00:00Z","timestamp":1668211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grant Agency of Czech Republic","award":["21-17044S"],"award-info":[{"award-number":["21-17044S"]}]},{"name":"Grant Agency of Czech Republic","award":["SGS01-2021"],"award-info":[{"award-number":["SGS01-2021"]}]},{"name":"Grant Agency of Czech Republic","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/000739"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/000739"]}]},{"name":"Internal Grant Agency of University of Ostrava","award":["21-17044S"],"award-info":[{"award-number":["21-17044S"]}]},{"name":"Internal Grant Agency of University of Ostrava","award":["SGS01-2021"],"award-info":[{"award-number":["SGS01-2021"]}]},{"name":"Internal Grant Agency of University of Ostrava","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/000739"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/000739"]}]},{"name":"Ministry of Education, Youth and Sport of the Czech Republic","award":["21-17044S"],"award-info":[{"award-number":["21-17044S"]}]},{"name":"Ministry of Education, Youth and Sport of the Czech Republic","award":["SGS01-2021"],"award-info":[{"award-number":["SGS01-2021"]}]},{"name":"Ministry of Education, Youth and Sport of the Czech Republic","award":["CZ.02.1.01\/0.0\/0.0\/16_025\/000739"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_025\/000739"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The multiple forms of pulmonary aspergillosis caused by Aspergillus species are the most common respiratory mycoses. Although invasive, the analysis of diagnostic biomarkers in bronchoalveolar lavage fluid (BALF) is a clinical standard for diagnosing these conditions. The BALF samples from 22 patients with proven or probable aspergillosis were assayed for human pentraxin 3 (Ptx3), fungal ferricrocin (Fc), and triacetylfusarinine C (TafC) in a retrospective study. The infected group included patients with invasive pulmonary aspergillosis (IPA) and chronic aspergillosis (CPA). The BALF data were compared to a control cohort of 67 patients with invasive pulmonary mucormycosis (IPM), non-Aspergillus colonization, or bacterial infections. The median Ptx3 concentrations in patients with and without aspergillosis were 4545.5 and 242.0 pg\/mL, respectively (95% CI, p &lt; 0.05). The optimum Ptx3 cutoff for IPA was 2545 pg\/mL, giving a sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 100, 98, 95, and 100%, respectively. The median Ptx3 concentration for IPM was high at 4326 pg\/mL. Pentraxin 3 assay alone can distinguish IPA from CPA and invasive fungal disease from colonization. Combining Ptx3 and TafC assays enabled the diagnostic discrimination of IPM and IPA, giving a specificity and PPV of 100%.<\/jats:p>","DOI":"10.3390\/jof8111194","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T02:26:37Z","timestamp":1668392797000},"page":"1194","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Distinguishing Invasive from Chronic Pulmonary Infections: Host Pentraxin 3 and Fungal Siderophores in Bronchoalveolar Lavage Fluids"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4299-9169","authenticated-orcid":false,"given":"Radim","family":"Dobi\u00e1\u0161","sequence":"first","affiliation":[{"name":"Department of Bacteriology and Mycology, National Reference Laboratory for Mycological Diagnostics, Public Health Institute in Ostrava, 70200 Ostrava, Czech Republic"},{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava, 70300 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavla","family":"Jaworsk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Mycology, National Reference Laboratory for Mycological Diagnostics, Public Health Institute in Ostrava, 70200 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7466-7535","authenticated-orcid":false,"given":"Valeria","family":"Skopelidou","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava, 70300 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Strako\u0161","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava, 70300 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6060-9454","authenticated-orcid":false,"given":"Denisa","family":"Vi\u0161\u0148ovsk\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava, 70300 Ostrava, Czech Republic"},{"name":"Department of Biology and Ecology, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8880-8925","authenticated-orcid":false,"given":"Marcela","family":"K\u00e1\u0148ov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine, University Hospital Ostrava, 70800 Ostrava, Czech Republic"},{"name":"Institute of Physiology and Pathophysiology, Faculty of Medicine, University of Ostrava, 71000 Ostrava, Czech Republic"},{"name":"Department of Intensive Medicine, Emergency Medicine and Forensic Studies, University of Ostrava, 71000 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1122-1098","authenticated-orcid":false,"given":"Anton","family":"\u0160kr\u00edba","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavl\u00edna","family":"Lyskov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Prague and Kladno, Public Health Institute in \u00dast\u00ed nad Labem, 18600 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1\u0161","family":"Bartek","sequence":"additional","affiliation":[{"name":"Department of Lung Disease and Tuberculosis, University Hospital Ostrava, 70800 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Jan\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Lung Disease and Tuberculosis, University Hospital Ostrava, 70800 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radovan","family":"Kozel","sequence":"additional","affiliation":[{"name":"Department of Pneumology and Phthisiology, Ostrava City Hospital, 72880 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Cwikov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pneumology and Phthisiology, Ostrava City Hospital, 72880 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbyn\u011bk","family":"Vrba","sequence":"additional","affiliation":[{"name":"Lung Department, Krnov Combined Medical Facility, 79401 Krnov, Czech 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Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Krej\u010d\u00ed","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Mycology, National Reference Laboratory for Mycological Diagnostics, Public Health Institute in Ostrava, 70200 Ostrava, Czech Republic"},{"name":"Institute of Laboratory Medicine, Faculty of Medicine, University of Ostrava, 70300 Ostrava, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5203-8276","authenticated-orcid":false,"given":"V\u00edt","family":"Ulmann","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Mycology, National Reference Laboratory for Mycological Diagnostics, Public Health Institute in Ostrava, 70200 Ostrava, Czech Republic"},{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515 Olomouc, Czech 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Sciences, 14220 Prague, Czech Republic"},{"name":"Department of Analytical Chemistry, Faculty of Science, Palack\u00fd University, 77146 Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S121","DOI":"10.1093\/cid\/ciaa1751","article-title":"EORTC\/MSGERC Definitions of Invasive Fungal Diseases: Summary of Activities of the Intensive Care Unit Working Group","volume":"72","author":"Bassetti","year":"2021","journal-title":"Clin. 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