{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:31:00Z","timestamp":1774467060896,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Invasive candidiasis (IC) contributes to the morbidity and mortality of hospitalized patients and represents a significant burden to the healthcare system. Previous Brazilian studies have reported the presence of endemic Candida parapsilosis sensu stricto genotypes causing candidemia and clonal transmission involving fluconazole-resistant isolates. We performed a 5-year retrospective analysis of IC cases in a Brazilian tertiary pediatric hospital and conducted a molecular investigation of C. parapsilosis sensu stricto. Non-duplicate C. parapsilosis sensu stricto genotyping was performed by microsatellite analysis. Antifungal susceptibility and biofilm formation were also evaluated. A total of 123 IC episodes were identified, with an IC incidence of 1.24 cases per 1000 hospital admissions and an overall mortality of 34%. The main species were the C. parapsilosis complex (35.8%), Candida albicans (29.2%), and Candida tropicalis (21.9%). All C. parapsilosis sensu stricto were recovered from blood cultures, and 97.5% were biofilm producers. Microsatellite typing identified high genotypic diversity among the isolates. We observed that all isolates were sensitive to amphotericin B, and although one isolate was non-sensitive to fluconazole, only a silent mutation on ERG11 gene was identified. No clear evidence of clonal outbreak or emergence of fluconazole-resistant isolates was found, suggesting that multiple sources may be involved in the epidemiology of IC in children.<\/jats:p>","DOI":"10.3390\/jof8121280","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T01:47:46Z","timestamp":1670377666000},"page":"1280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genotypic Diversity of Candida parapsilosis Complex in Invasive Candidiasis at a Pediatric Tertiary Hospital: A 5-Year Retrospective Study"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-8650","authenticated-orcid":false,"given":"Luiza S.","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriele C.","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regiane N.","family":"Spalanzani","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tha\u00eds M.","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Sestren","sequence":"additional","affiliation":[{"name":"Hospital Pequeno Pr\u00edncipe, Desembargador Motta, 1070-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saloe P.","family":"Bispo","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata B. V.","family":"Abreu","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Let\u00edcia","family":"Kraft","sequence":"additional","affiliation":[{"name":"Coordena\u00e7\u00e3o-Geral de Laborat\u00f3rios de Sa\u00fade P\u00fablica, do Departamento de Articula\u00e7\u00e3o Estrat\u00e9gica de Vigil\u00e2ncia Em Sa\u00fade, da Secert\u00e1ria de Vigil\u00e2ncia em Sa\u00fade do Minist\u00e9rio da Sa\u00fade, SRTVN 701, Via W5 Norte, Bras\u00edlia CEP 70719-040, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marinei C.","family":"Ricieri","sequence":"additional","affiliation":[{"name":"Hospital Pequeno Pr\u00edncipe, Desembargador Motta, 1070-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6241-0302","authenticated-orcid":false,"given":"F\u00e1bio A.","family":"Motta","sequence":"additional","affiliation":[{"name":"Hospital Pequeno Pr\u00edncipe, Desembargador Motta, 1070-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9636-1817","authenticated-orcid":false,"given":"Libera M.","family":"Dalla-Costa","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Av. Silva Jardim, 1632-\u00c1gua Verde, Curitiba CEP 80250-060, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Riera, F.O., Caeiro, J.P., Angiolini, S.C., Vigezzi, C., Rodriguez, E., Icely, P.A., and Sotomayor, C.E. (2022). Invasive Candidiasis: Update and current challenges in the management of this mycosis in South America. Antibiotics, 11.","DOI":"10.3390\/antibiotics11070877"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"27099","DOI":"10.1038\/srep27099","article-title":"Investigation of an unrecognized large-scale outbreak of Candida Parapsilosis sensu stricto fungaemia in a tertiary-care hospital in China","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. 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