{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T07:12:48Z","timestamp":1775113968085,"version":"3.50.1"},"reference-count":79,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Cryptococcosis, caused predominantly by Cryptococcus neoformans, is a potentially fatal, opportunistic infection that commonly affects the central nervous system of immunocompromised patients. Globally, this mycosis is responsible for almost 20% of AIDS-related deaths, and in countries like Peru, its incidence remains high, mostly due to the annual increase in new cases of HIV infection. This study aimed to establish the genotypic diversity and antifungal susceptibility of C.\u00a0neoformans isolates causing meningoencephalitis in 25 adults and a 9-year-old girl with HIV and other risk factors from Lima, Peru. To identify the genotype of the isolates, multilocus sequence typing was applied, and to establish the susceptibility of the isolates to six antifungals, a YeastOne\u00ae broth microdilution was used. From the isolates, 19 were identified as molecular type VNI, and seven as VNII, grouped in eight and three sequence types, respectively, which shows that the studied population was highly diverse. Most isolates were susceptible to all antifungals tested. However, VNI isolates were less susceptible to fluconazole, itraconazole and voriconazole than VNII isolates (p &lt; 0.05). This study contributes data on the molecular epidemiology and the antifungal susceptibility profile of the most common etiological agent of cryptococcosis, highlighting a pediatric case, something which is rare among cryptococcal infection.<\/jats:p>","DOI":"10.3390\/jof8121306","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T04:54:13Z","timestamp":1671425653000},"page":"1306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cryptococcus neoformans Causing Meningoencephalitis in Adults and a Child from Lima, Peru: Genotypic Diversity and Antifungal Susceptibility"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7547-5172","authenticated-orcid":false,"given":"Carolina","family":"Firacative","sequence":"first","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogota 111221, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Zuluaga-Puerto","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Universidad de Rosario, Bogota 111221, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Guevara","sequence":"additional","affiliation":[{"name":"Facultad de Medicina \u201cSan Fernando\u201d, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"a019760","DOI":"10.1101\/cshperspect.a019760","article-title":"Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis","volume":"4","author":"Fraser","year":"2014","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Heitman, J., Kozel, T.R., Kwon-Chung, J.K., Perfect, J.R., and Casadevall, A. (2011). Environmental Niches for Cryptococcus neoformans and Cryptococcus gattii. Cryptococcus: From Human Pathogen to Model Yeast, ASM Press.","DOI":"10.1128\/9781555816858"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/CMR.8.4.515","article-title":"Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans","volume":"8","author":"Mitchell","year":"1995","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/S1473-3099(22)00499-6","article-title":"The global burden of HIV-associated cryptococcal infection in adults in 2020: A modelling analysis","volume":"22","author":"Rajasingham","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","first-page":"61","article-title":"Cryptococcal infections in non-HIV-infected patients","volume":"124","author":"Pappas","year":"2013","journal-title":"Trans. Am. Clin. Climatol. Assoc."},{"key":"ref_6","unstructured":"UNAIDS (2022, February 04). UNAIDS. Available online: https:\/\/www.unaids.org\/en\/regionscountries\/countries\/peru."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.prp.2006.07.005","article-title":"Postmortem findings and opportunistic infections in HIV-positive patients from a public hospital in Peru","volume":"202","author":"Eza","year":"2006","journal-title":"Pathol. Res. Pract."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1080\/13693780902953886","article-title":"Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii","volume":"47","author":"Meyer","year":"2009","journal-title":"Med. Mycol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.riam.2014.01.005","article-title":"Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru","volume":"32","author":"Bejar","year":"2015","journal-title":"Rev. Iberoam. De Micol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Van de Wiele, N., Neyra, E., Firacative, C., Gilgado, F., Serena, C., Bustamante, B., and Meyer, W. (2020). Molecular epidemiology reveals low genetic diversity among Cryptococcus neoformans isolates from people living with HIV in Lima, Peru, during the Pre-HAART Era. Pathogens, 9.","DOI":"10.3390\/pathogens9080665"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e28802","DOI":"10.7554\/eLife.28802","article-title":"Natural mismatch repair mutations mediate phenotypic diversity and drug resistance in Cryptococcus deuterogattii","volume":"6","author":"Billmyre","year":"2017","journal-title":"eLife"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1111\/mmi.14565","article-title":"Combatting the evolution of antifungal resistance in Cryptococcus neoformans","volume":"114","author":"Bermas","year":"2020","journal-title":"Mol. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"140135","DOI":"10.1016\/j.scitotenv.2020.140135","article-title":"Hypervirulence and cross-resistance to a clinical antifungal are induced by an environmental fungicide in Cryptococcus gattii","volume":"740","author":"Carneiro","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/s41579-022-00720-1","article-title":"Tackling the emerging threat of antifungal resistance to human health","volume":"20","author":"Fisher","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Firacative, C., Meyer, W., and Casta\u00f1eda, E. (2021). Cryptococcus neoformans and Cryptococcus gattii species complexes in Latin America: A map of molecular types, genotypic diversity, and antifungal susceptibility as reported by the Latin American Cryptococcal Study Group. J. Fungi, 7.","DOI":"10.3390\/jof7040282"},{"key":"ref_16","unstructured":"Gonz\u00e1lez, A., G\u00f3mez, B.L., Tob\u00f3n, A., and Restrepo, A. (2018). Criptococosis. Fundamentos de las Micosis Humanas, CIB-Universidad de Antioquia. [1st ed.]."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/IAI.66.1.89-97.1998","article-title":"Microevolution of a standard strain of Cryptococcus neoformans resulting in differences in virulence and other phenotypes","volume":"66","author":"Franzot","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1534\/genetics.105.046672","article-title":"Multilocus sequence typing reveals three genetic subpopulations of Cryptococcus neoformans var. grubii (serotype A), including a unique population in Botswana","volume":"172","author":"Litvintseva","year":"2006","journal-title":"Genetics"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Andrade-Silva, L.E., Ferreira-Paim, K., Ferreira, T.B., Vilas-Boas, A., Mora, D.J., Manzato, V.M., Fonseca, F.M., Buosi, K., Andrade-Silva, J., and Prudente, B.D.S. (2018). Genotypic analysis of clinical and environmental Cryptococcus neoformans isolates from Brazil reveals the presence of VNB isolates and a correlation with biological factors. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193237"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ferreira-Paim, K., Andrade-Silva, L., Fonseca, F.M., Ferreira, T.B., Mora, D.J., Andrade-Silva, J., Khan, A., Dao, A., Reis, E.C., and Almeida, M.T. (2017). MLST-based population genetic analysis in a global context reveals clonality amongst Cryptococcus neoformans var. grubii VNI isolates from HIV patients in Southeastern Brazil. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005223"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rocha, D.F.S., Cruz, K.S., Santos, C., Menescal, L.S.F., Neto, J., Pinheiro, S.B., Silva, L.M., Trilles, L., and Braga de Souza, J.V. (2018). MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0197841"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.fgb.2016.01.003","article-title":"Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population","volume":"87","author":"Cogliati","year":"2016","journal-title":"Fungal Genet. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Velez, N., Alvarado, M., Parra-Giraldo, C.M., Sanchez-Quitian, Z.A., Escandon, P., and Castaneda, E. (2018). Genotypic diversity is independent of pathogenicity in Colombian strains of Cryptococcus neoformans and Cryptococcus gattii in Galleria mellonella. J. Fungi, 4.","DOI":"10.3390\/jof4030082"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Firacative, C., Roe, C.C., Malik, R., Ferreira-Paim, K., Escandon, P., Sykes, J.E., Castanon-Olivares, L.R., Contreras-Peres, C., Samayoa, B., and Sorrell, T.C. (2016). MLST and whole-genome-based population analysis of Cryptococcus gattii VGIII links clinical, veterinary and environmental strains, and reveals divergent serotype specific sub-populations and distant ancestors. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004861"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1093\/molbev\/msx248","article-title":"DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets","volume":"34","author":"Rozas","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1128\/JCM.37.3.591-595.1999","article-title":"Multicenter comparison of the sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory standards M27-A reference method for testing clinical isolates of common and emerging Candida spp., Cryptococcus spp., and other yeasts and yeast-like organisms","volume":"37","author":"Pfaller","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_28","unstructured":"(2022). Performance Standards for Antifungal Susceptibility Testing of Yeasts (Standard No. CLSI supplement M27M44S)."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5898","DOI":"10.1128\/AAC.01115-12","article-title":"Cryptococcus neoformans-Cryptococcus gattii species complex: An international study of wild-type susceptibility endpoint distributions and epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole","volume":"56","author":"Aller","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3107","DOI":"10.1128\/AAC.06252-11","article-title":"Cryptococcus neoformans-Cryptococcus gattii species complex: An international study of wild-type susceptibility endpoint distributions and epidemiological cutoff values for amphotericin B and flucytosine","volume":"56","author":"Chowdhary","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Delma, F.Z., Al-Hatmi, A.M.S., Buil, J.B., van der Lee, H., Tehupeiory-Kooreman, M., de Hoog, G.S., Meletiadis, J., and Verweij, P.E. (2020). Comparison of MIC test strip and Sensititre YeastOne with the CLSI and EUCAST broth microdilution reference methods for in vitro antifungal susceptibility testing of Cryptococcus neoformans. Antimicrob. Agents Chemother., 64.","DOI":"10.1128\/AAC.02261-19"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1097\/QAD.0b013e3283570567","article-title":"A comparison of cases of paediatric-onset and adult-onset cryptococcosis detected through population-based surveillance, 2005\u20132007","volume":"26","author":"Meiring","year":"2012","journal-title":"AIDS"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1590\/0074-0276130537","article-title":"Cryptococcosis in Colombian children and literature review","volume":"109","author":"Lizarazo","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e170554","DOI":"10.1590\/0074-02760170554","article-title":"The status of cryptococcosis in Latin America","volume":"113","author":"Firacative","year":"2018","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e20180419","DOI":"10.1590\/0037-8682-0419-2018","article-title":"First case report of cryptococcosis due to Cryptococcus decagattii in a pediatric patient in Argentina","volume":"52","author":"Berejnoi","year":"2019","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1590\/S0037-86822010000600032","article-title":"Cryptococcus gattii molecular type VGII as agent of meningitis in a healthy child in Rio de Janeiro, Brazil: Report of an autochthonous case","volume":"43","author":"Pone","year":"2010","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1590\/S0074-02762011000600012","article-title":"Genotypes of Cryptococcus neoformans and Cryptococcus gattii as agents of endemic cryptococcosis in Teresina, Piaui (northeastern Brazil)","volume":"106","author":"Martins","year":"2011","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.3855\/jidc.9946","article-title":"Fatal neurocryptococcosis in a Colombian underage patient","volume":"13","author":"Noguera","year":"2019","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1128\/JCM.03177-13","article-title":"Multilocus sequence typing of serially collected isolates of Cryptococcus from HIV-infected patients in South Africa","volume":"52","author":"Govender","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","unstructured":"ISHAM (2022, November 22). Genotyping of Cryptococcus neoformans and C. gattii. Available online: https:\/\/www.isham.org\/working-groups\/genotyping-cryptococcous-neoformans-and-c-gattii."},{"key":"ref_41","first-page":"e00311","article-title":"Cryptococcosis Serotypes Impact Outcome and Provide Evidence of Cryptococcus neoformans Speciation","volume":"6","author":"Patel","year":"2015","journal-title":"mBio"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s00430-014-0341-6","article-title":"Molecular typing of clinical Cryptococcus neoformans isolates collected in Germany from 2004 to 2010","volume":"203","author":"Sanchini","year":"2014","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1038\/s41598-018-20054-4","article-title":"Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China","volume":"8","author":"Chen","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"252","DOI":"10.3109\/13693786.2012.708883","article-title":"Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan","volume":"51","author":"Mihara","year":"2013","journal-title":"Med. Mycol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"51","DOI":"10.7883\/yoken.66.51","article-title":"Determination of epidemiology of clinically isolated Cryptococcus neoformans strains in Japan by multilocus sequence typing","volume":"66","author":"Umeyama","year":"2013","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Khayhan, K., Hagen, F., Pan, W., Simwami, S., Fisher, M.C., Wahyuningsih, R., Chakrabarti, A., Chowdhary, A., Ikeda, R., and Taj-Aldeen, S.J. (2013). Geographically structured populations of Cryptococcus neoformans variety grubii in Asia correlate with HIV status and show a clonal population structure. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0072222"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Hatthakaroon, C., Pharkjaksu, S., Chongtrakool, P., Suwannakarn, K., Kiratisin, P., and Ngamskulrungroj, P. (2017). Molecular epidemiology of cryptococcal genotype VNIc\/ST5 in Siriraj Hospital, Thailand. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0173744"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Day, J.N., Qihui, S., Thanh, L.T., Trieu, P.H., Van, A.D., Thu, N.H., Chau, T.T.H., Lan, N.P.H., Chau, N.V.V., and Ashton, P.M. (2017). Comparative genomics of Cryptococcus neoformans var. grubii associated with meningitis in HIV infected and uninfected patients in Vietnam. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005628"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3559","DOI":"10.1111\/mec.13260","article-title":"Comparative analyses of clinical and environmental populations of Cryptococcus neoformans in Botswana","volume":"24","author":"Chen","year":"2015","journal-title":"Mol. Ecol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1128\/JCM.03392-13","article-title":"Antifungal drug susceptibility and phylogenetic diversity among Cryptococcus isolates from dogs and cats in North America","volume":"52","author":"Singer","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.fgb.2019.04.001","article-title":"Genotypes and population genetics of Cryptococcus neoformans and Cryptococcus gattii species complexes in Europe and the mediterranean area","volume":"129","author":"Cogliati","year":"2019","journal-title":"Fungal Genet. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1111\/1567-1364.12178","article-title":"Multilocus sequence typing (MLST) and M13 PCR fingerprinting revealed heterogeneity amongst Cryptococcus species obtained from Italian veterinary isolates","volume":"14","author":"Danesi","year":"2014","journal-title":"FEMS Yeast Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/myc.13041","article-title":"Genotypic diversity in clinical and environmental isolates of Cryptococcus neoformans from India using multilocus microsatellite and multilocus sequence typing","volume":"63","author":"Prakash","year":"2020","journal-title":"Mycoses"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"73","DOI":"10.5941\/MYCO.2014.42.1.73","article-title":"Molecular epidemiology of clinical Cryptococcus neoformans isolates in Seoul, Korea","volume":"42","author":"Park","year":"2014","journal-title":"Mycobiology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1093\/mmy\/myy105","article-title":"Multilocus sequence typing of Cryptococcus neoformans var. grubii from Laos in a regional and global context","volume":"57","author":"Thanh","year":"2018","journal-title":"Med. Mycol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"360","DOI":"10.5941\/MYCO.2015.43.3.360","article-title":"Genotypes of Clinical and Environmental Isolates of Cryptococcus neoformans and Cryptococcus gattii in Korea","volume":"43","author":"Park","year":"2015","journal-title":"Mycobiology"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Kassi, F.K., Drakulovski, P., Bellet, V., Roger, F., Chabrol, A., Krasteva, D., Doumbia, A., Landman, R., Kakou, A., and Reynes, J. (2019). Cryptococcus genetic diversity and mixed infections in Ivorian HIV patients: A follow up study. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007812"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Beale, M.A., Sabiiti, W., Robertson, E.J., Fuentes-Cabrejo, K.M., O\u2019Hanlon, S.J., Jarvis, J.N., Loyse, A., Meintjes, G., Harrison, T.S., and May, R.C. (2015). Genotypic Diversity Is Associated with Clinical Outcome and Phenotype in Cryptococcal Meningitis across Southern Africa. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003847"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"887.e881","DOI":"10.1016\/j.cmi.2016.07.008","article-title":"Predominance of Cryptococcus neoformans var. grubii multilocus sequence type 5 and emergence of isolates with non-wild-type minimum inhibitory concentrations to fluconazole: A multi-centre study in China","volume":"22","author":"Fan","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"499","DOI":"10.3109\/13693786.2012.751642","article-title":"Molecular epidemiology of Italian clinical Cryptococcus neoformans var. grubii isolates","volume":"51","author":"Cogliati","year":"2013","journal-title":"Med. Mycol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wiesner, D.L., Moskalenko, O., Corcoran, J.M., McDonald, T., Rolfes, M.A., Meya, D.B., Kajumbula, H., Kambugu, A., Bohjanen, P.R., and Knight, J.F. (2012). Cryptococcal genotype influences immunologic response and human clinical outcome after meningitis. mBio, 3.","DOI":"10.1128\/mBio.00196-12"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1093\/mmy\/myx026","article-title":"Molecular characterization of Cryptococcus neoformans isolated from the environment in Beijing, China","volume":"55","author":"Dou","year":"2017","journal-title":"Med. Mycol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Simwami, S.P., Khayhan, K., Henk, D.A., Aanensen, D.M., Boekhout, T., Hagen, F., Brouwer, A.E., Harrison, T.S., Donnelly, C.A., and Fisher, M.C. (2011). Low diversity Cryptococcus neoformans variety grubii multilocus sequence types from Thailand are consistent with an ancestral African origin. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1001343"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1093\/mmy\/myy022","article-title":"Molecular-type specific multiplex PCR produces a distinct VNII PCR pattern among Cryptococcus neoformans species complex","volume":"57","author":"Cogliati","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s11046-019-00376-1","article-title":"Whole-genome sequencing of an uncommon Cryptococcus neoformans MLST43 genotype isolated in Nigeria","volume":"184","author":"Nnadi","year":"2019","journal-title":"Mycopathologia"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Kaocharoen, S., Ngamskulrungroj, P., Firacative, C., Trilles, L., Piyabongkarn, D., Banlunara, W., Poonwan, N., Chaiprasert, A., Meyer, W., and Chindamporn, A. (2013). Molecular epidemiology reveals genetic diversity amongst isolates of the Cryptococcus neoformans\/C. gattii species complex in Thailand. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002297"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Reis, R.S., Bonna, I.C.F., Antonio, I., Pereira, S.A., Nascimento, C., Ferraris, F.K., Brito-Santos, F., Ferreira Gremiao, I.D., and Trilles, L. (2021). Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil. J. Fungi, 7.","DOI":"10.3390\/jof7110980"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.jinf.2008.06.020","article-title":"Treatment of cryptococcal meningitis in Peruvian AIDS patients using amphotericin B and fluconazole","volume":"57","author":"Dammert","year":"2008","journal-title":"J. Infect."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Concha-Velasco, F., Gonzalez-Lagos, E., Seas, C., and Bustamante, B. (2017). Factors associated with early mycological clearance in HIV-associated cryptococcal meningitis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0174459"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1054\/drup.1999.0090","article-title":"Drug resistance in Cryptococcus neoformans","volume":"2","author":"Perfect","year":"1999","journal-title":"Drug Resist. Updates"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/myc.13389","article-title":"Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents","volume":"65","author":"Bellet","year":"2022","journal-title":"Mycoses"},{"key":"ref_72","unstructured":"Padda, I.S., and Parmar, M. (2022). Flucytosine. StatPearls, StatPearls Publishing."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"4115","DOI":"10.1128\/JCM.01271-10","article-title":"In vitro susceptibility of the yeast pathogen Cryptococcus to fluconazole and other azoles varies with molecular genotype","volume":"48","author":"Chong","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1093\/mmy\/myab041","article-title":"Antifungal susceptibility of clinical Cryptococcus gattii isolates from Colombia varies among molecular types","volume":"59","author":"Firacative","year":"2021","journal-title":"Med. Mycol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.diagmicrobio.2012.02.018","article-title":"Epidemiologic cutoff values for triazole drugs in Cryptococcus gattii: Correlation of molecular type and in vitro susceptibility","volume":"73","author":"Lockhart","year":"2012","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3109\/13693786.2011.602126","article-title":"Correlation of antifungal susceptibility and molecular type within the Cryptococcus neoformans\/C. gattii species complex","volume":"50","author":"Trilles","year":"2012","journal-title":"Med. Mycol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"675213","DOI":"10.1155\/2013\/675213","article-title":"Global molecular epidemiology of Cryptococcus neoformans and Cryptococcus gattii: An atlas of the molecular types","volume":"2013","author":"Cogliati","year":"2013","journal-title":"Scientifica"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1093\/mmy\/myy161","article-title":"Molecular types of Cryptococcus neoformans and Cryptococcus gattii in Western Australia and correlation with antifungal susceptibility","volume":"57","author":"Lee","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/myc.12507","article-title":"Molecular epidemiology and in vitro antifungal susceptibility testing of 108 clinical Cryptococcus neoformans sensu lato and Cryptococcus gattii sensu lato isolates from Denmark","volume":"59","author":"Hagen","year":"2016","journal-title":"Mycoses"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/12\/1306\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:42:47Z","timestamp":1760146967000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/8\/12\/1306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,16]]},"references-count":79,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["jof8121306"],"URL":"https:\/\/doi.org\/10.3390\/jof8121306","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,16]]}}}