{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:56:32Z","timestamp":1762520192126,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T00:00:00Z","timestamp":1674518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003086","name":"Basque Government","doi-asserted-by":"publisher","award":["IT1362-19","IT1657-22"],"award-info":[{"award-number":["IT1362-19","IT1657-22"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The detection of Scedosporium\/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium\/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium\/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium\/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium\/Lomentospora in patients with CF.<\/jats:p>","DOI":"10.3390\/jof9020158","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T03:23:49Z","timestamp":1674617029000},"page":"158","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium\/Lomentospora in Cystic Fibrosis Patients"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8540-5629","authenticated-orcid":false,"given":"Leire","family":"Martin-Souto","sequence":"first","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2594-8220","authenticated-orcid":false,"given":"Aitziber","family":"Antoran","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maialen","family":"Areitio","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9994-6602","authenticated-orcid":false,"given":"Leire","family":"Aparicio-Fernandez","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8119-8749","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"Mart\u00edn-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Microbiology Department, Vall d\u2019Hebron University Hospital, 08035 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Department of Research and Development, IMG Pharma Biotech S.L., 48160 Derio, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0338-5554","authenticated-orcid":false,"given":"Egoitz","family":"Astigarraga","sequence":"additional","affiliation":[{"name":"Department of Research and Development, IMG Pharma Biotech S.L., 48160 Derio, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8330-1567","authenticated-orcid":false,"given":"Gabriel","family":"Barreda-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Research and Development, IMG Pharma Biotech S.L., 48160 Derio, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Division of Cystic Fibrosis, CF Center Westbrandenburg, Campus Potsdam, Klinikum Westbrandenburg, 14467 Potsdam, Germany"},{"name":"Faculty of Medicine, HMU-Health and Medical University Potsdam, 14471 Potsdam, Germany"},{"name":"Charit\u00e9-Universitaetsmedizin Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6154-4920","authenticated-orcid":false,"given":"Volker","family":"Rickerts","sequence":"additional","affiliation":[{"name":"Division for Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute Berlin, 13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando L.","family":"Hernando","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0478-1038","authenticated-orcid":false,"given":"Aitor","family":"Rementeria","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Idoia","family":"Buldain","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2696-162X","authenticated-orcid":false,"given":"Andoni","family":"Ramirez-Garcia","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1128\/CMR.00068-09","article-title":"The Changing Microbial Epidemiology in Cystic Fibrosis","volume":"23","author":"LiPuma","year":"2010","journal-title":"Clin. 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