{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T11:30:44Z","timestamp":1763811044690,"version":"build-2065373602"},"reference-count":76,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Histoplasma capsulatum is a thermodymorphic fungus that causes histoplasmosis, a systemic mycosis that presents different clinical manifestations, ranging from self-limiting to acute lung infection, chronic lung infection and disseminated infection. Usually, it affects severely immunocompromised patients although immunocompetent patients can also be infected. Currently, there are no vaccines to prevent histoplasmosis and the available antifungal treatment presents moderate to high toxicity. Additionally, there are few options of antifungal drugs. Thus, the aim of this study was to predict possible protein targets for the construction of potential vaccine candidates and predict potential drug targets against H. capsulatum. Whole genome sequences from four previously published H. capsulatum strains were analyzed and submitted to different bioinformatic approaches such as reverse vaccinology and subtractive genomics. A total of four proteins were characterized as good protein candidates (vaccine antigens) for vaccine development, three of which are membrane-bound and one is secreted. In addition, it was possible to predict four cytoplasmic proteins which were classified as good protein candidates and, through molecular docking performed for each identified target, we found four natural compounds that showed favorable interactions with our target proteins. Our study can help in the development of potential vaccines and new drugs that can change the current scenario of the treatment and prevention of histoplasmosis.<\/jats:p>","DOI":"10.3390\/jof9020193","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T03:47:33Z","timestamp":1675309653000},"page":"193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparative Genomics of Histoplasma capsulatum and Prediction of New Vaccines and Drug Targets"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6708-4785","authenticated-orcid":false,"given":"Paula Cristina Silva","family":"Almeida","sequence":"first","affiliation":[{"name":"Program of Multi-Professional Residence, Federal University of Triangulo Mineiro, Uberaba 38025-440, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Stelmastchuk","family":"Roque","sequence":"additional","affiliation":[{"name":"Program of Multi-Professional Residence, Federal University of Triangulo Mineiro, Uberaba 38025-440, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8813-8850","authenticated-orcid":false,"given":"Andrei Giacchetto","family":"Felice","sequence":"additional","affiliation":[{"name":"Post-Graduate Program in Tropical Medicine and Infectology, Federal University of Triangulo Mineiro, Uberaba 38025-440, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0558-483X","authenticated-orcid":false,"given":"Arun Kumar","family":"Jaiswal","sequence":"additional","affiliation":[{"name":"Post-Graduate Program in Bioinformatics, Federal University of Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8554-1660","authenticated-orcid":false,"given":"Sandeep","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Post-Graduate Program in Microbiology, Institute of Biology, Federal University of Bahia, Salvador 40170-110, Brazil"},{"name":"Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador 40231-300, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-2280","authenticated-orcid":false,"given":"Vasco","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Le\u00f3n","family":"Silva-Vergara","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Federal University of Triangulo Mineiro, Uberaba 38025-440, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7299-3724","authenticated-orcid":false,"given":"Siomar","family":"de Castro Soares","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of Triangulo Mineiro, Uberaba 38015-05, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6035-8392","authenticated-orcid":false,"given":"Kennio","family":"Ferreira-Paim","sequence":"additional","affiliation":[{"name":"Program of Multi-Professional Residence, Federal University of Triangulo Mineiro, Uberaba 38025-440, Brazil"},{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of Triangulo Mineiro, Uberaba 38015-05, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5326-0776","authenticated-orcid":false,"given":"Fernanda Machado","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Program of Multi-Professional Residence, Federal University of Triangulo Mineiro, Uberaba 38025-440, Brazil"},{"name":"Department of Biomedicine, Federal University of Triangulo Mineiro, Uberaba 38025-350, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"892","DOI":"10.2307\/4587021","article-title":"Isolation of Histoplasma capsulatum from soil","volume":"64","author":"Emmons","year":"1949","journal-title":"Public Health Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1093\/oxfordjournals.aje.a120973","article-title":"Isolation of Histoplasma capsulatum from Arizona bats","volume":"89","author":"Ajello","year":"1969","journal-title":"Am. 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