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One of the defense mechanisms used by the host includes the sequestration of metals used in fungal metabolism, such as iron and zinc. There are several mechanisms that maintain the balance in the intracellular zinc supply. MicroRNAs are effector molecules of responses between the pathogen and host, favoring or preventing infection in many microorganisms. Fungi of the Paracoccidioides genus are thermodimorphic and the etiological agents of paracoccidioidomycosis (PCM). In the current pandemic scenario world mycosis studies continue to be highly important since a significant number of patients with COVID-19 developed systemic mycoses, co-infections that complicated their clinical condition. The objective was to identify transcriptomic and proteomic adaptations in Paracoccidioides brasiliensis during zinc deprivation. Nineteen microRNAs were identified, three of which were differentially regulated. Target genes regulated by those microRNAs are elements of zinc homeostasis such as ZRT1, ZRT3 and COT1 transporters. Transcription factors that have zinc in their structure are also targets of those miRNAs. Transcriptional and proteomic data suggest that P. brasiliensis undergoes metabolic remodeling to survive zinc deprivation and that miRNAs may be part of the regulatory process.<\/jats:p>","DOI":"10.3390\/jof9030281","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T02:31:55Z","timestamp":1677033115000},"page":"281","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Global Molecular Response of Paracoccidioides brasiliensis to Zinc Deprivation: Analyses at Transcript, Protein and MicroRNA Levels"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7123-1127","authenticated-orcid":false,"given":"Lorena Alves","family":"Mesquita","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7754-4047","authenticated-orcid":false,"given":"Alexandre Melo","family":"Bail\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Santana","family":"de Curcio","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kassyo Lobato Potenciano","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4897-8521","authenticated-orcid":false,"given":"Gabriel","family":"da Rocha Fernandes","sequence":"additional","affiliation":[{"name":"Inform\u00e1tica de Biossistemas & Gen\u00f4mica, Fiocruz Minas, Belo Horizonte 30190-009, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2053-204X","authenticated-orcid":false,"given":"Mirelle Garcia","family":"Silva-Bail\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3803-0339","authenticated-orcid":false,"given":"Evandro","family":"Novaes","sequence":"additional","affiliation":[{"name":"Setor de Gen\u00e9tica, Departamento de Biologia, Universidade Federal de Lavras, Lavras 37203-202, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5378-9840","authenticated-orcid":false,"given":"C\u00e9lia Maria","family":"de Almeida Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1007\/s00775-008-0404-5","article-title":"Metal Ions in Biological Catalysis: From Enzyme Databases to General Principles","volume":"13","author":"Andreini","year":"2008","journal-title":"J. Biol. Inorg. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1089\/ars.2006.8.1419","article-title":"Zinc Coordination Environments in Proteins as Redox Sensors and Signal Transducers","volume":"8","author":"Maret","year":"2006","journal-title":"Antioxid. Redox Signal."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1093\/jn\/137.4.1101","article-title":"Zinc Deficiency: A Special Challenge","volume":"137","author":"Hambidge","year":"2007","journal-title":"J. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Barth, L.M., Rink, L., and Wessels, I. (2020). Increase of the Intracellular Zinc Concentration Leads to an Activation and Internalisation of the Epidermal Growth Factor Receptor in A549 Cells. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22010326"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00204-005-0009-5","article-title":"Zinc: A Multipurpose Trace Element","volume":"80","author":"Stefanidou","year":"2005","journal-title":"Arch. Toxicol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1042\/bj3460329","article-title":"Zinc-Regulated Ubiquitin Conjugation Signals Endocytosis of the Yeast ZRT1 Zinc Transporter","volume":"346","author":"Gitan","year":"2000","journal-title":"Biochem. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1534\/g3.119.400933","article-title":"Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in Saccharomyces Cerevisiae","volume":"10","author":"Zhao","year":"2020","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.lab.2004.07.007","article-title":"Fungal Susceptibility to Zinc Deprivation","volume":"144","author":"Lulloff","year":"2004","journal-title":"J. Lab. Clin. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s12281-014-0180-3","article-title":"Essential Metals in Cryptococcus Neoformans: Acquisition and Regulation","volume":"8","author":"Ding","year":"2014","journal-title":"Curr. Fungal Infect. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.abb.2016.02.020","article-title":"Immunological Orchestration of Zinc Homeostasis: The Battle between Host Mechanisms and Pathogen Defenses","volume":"611","author":"Deepe","year":"2016","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1146\/annurev.bi.55.070186.004405","article-title":"Metallothionein","volume":"55","author":"Hamer","year":"1986","journal-title":"Annu. Rev. Biochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1002\/j.1460-2075.1995.tb07042.x","article-title":"Cloning and Functional Characterization of a Mammalian Zinc Transporter That Confers Resistance to Zinc","volume":"14","author":"Palmiter","year":"1995","journal-title":"EMBO J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.bbamcr.2006.03.005","article-title":"Zinc Transporters and the Cellular Trafficking of Zinc","volume":"1763","author":"Eide","year":"2006","journal-title":"Biochim. Biophys. Acta BBA-Mol. Cell Res."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Soares, L.W., Bail\u00e3o, A.M., Soares, C.M.D.A., and Bail\u00e3o, M.G.S. (2020). Zinc at the Host\u2013Fungus Interface: How to Uptake the Metal?. J. Fungi, 6.","DOI":"10.3390\/jof6040305"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.1073\/pnas.93.6.2454","article-title":"The Yeast ZRT1 Gene Encodes the Zinc Transporter Protein of a High-Affinity Uptake System Induced by Zinc Limitation","volume":"93","author":"Zhao","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"23203","DOI":"10.1074\/jbc.271.38.23203","article-title":"The Gene Encodes the Low Affinity Zinc Transporter In","volume":"271","author":"Zhao","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1093\/emboj\/19.12.2845","article-title":"Zinc Transporters That Regulate Vacuolar Zinc Storage in Saccharomyces Cerevisiae","volume":"19","author":"MacDiarmid","year":"2000","journal-title":"EMBO J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1093\/mmy\/myw013","article-title":"The ZIP Family Zinc Transporters Support the Virulence of Cryptococcus Neoformans","volume":"54","author":"Do","year":"2016","journal-title":"Med. Mycol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10104","DOI":"10.1038\/srep10104","article-title":"Effects of Zinc Transporters on Cryptococcus Gattii Virulence","volume":"5","author":"Diehl","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1111\/cmi.12238","article-title":"The ZrfC Alkaline Zinc Transporter Is Required for Aspergillus Fumigatus Virulence and Its Growth in the Presence of the Zn\/Mn-Chelating Protein Calprotectin","volume":"16","author":"Amich","year":"2014","journal-title":"Cell. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Crawford, A.C., Lehtovirta-Morley, L.E., Alamir, O., Niemiec, M.J., Alawfi, B., Alsarraf, M., Skrahina, V., Costa, A.C.B.P., Anderson, A., and Yellagunda, S. (2018). Biphasic Zinc Compartmentalisation in a Human Fungal Pathogen. PLoS Pathog., 14.","DOI":"10.1371\/journal.ppat.1007013"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Citiulo, F., Jacobsen, I.D., Miram\u00f3n, P., Schild, L., Brunke, S., Zipfel, P., Brock, M., Hube, B., and Wilson, D. (2012). Candida Albicans Scavenges Host Zinc via Pra1 during Endothelial Invasion. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002777"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/BF00261172","article-title":"Identification of a Gene Conferring Resistance to Zinc and Cadmium Ions in the Yeast Saccharomyces Cerevisiae","volume":"219","author":"Kamizono","year":"1989","journal-title":"Mol. Gen. Genet. MGG"},{"key":"ref_24","first-page":"3678","article-title":"COT1, a Gene Involved in Cobalt Accumulation in Saccharomyces Cerevisiae","volume":"12","author":"Conklin","year":"1992","journal-title":"Mol. Cell. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1042\/BJ20102003","article-title":"Transcriptional Regulation of the Zrg17 Zinc Transporter of the Yeast Secretory Pathway","volume":"435","author":"Wu","year":"2011","journal-title":"Biochem. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3704","DOI":"10.1093\/emboj\/19.14.3704","article-title":"A Dual Role for Zinc Fingers in Both DNA Binding and Zinc Sensing by the Zap1 Transcriptional Activator","volume":"19","author":"Bird","year":"2000","journal-title":"EMBO J."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Wu, C.Y., Bird, A.J., Chung, L.M., Newton, M.A., Winge, D.R., and Eide, D.J. (2008). Differential Control of Zap1-Regulated Genes in Response to Zinc Deficiency in Saccharomyces Cerevisiae. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-370"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Vicentefranqueira, R., Amich, J., Mar\u00edn, L., S\u00e1nchez, C.I., Leal, F., and Calera, J.A. (2018). The Transcription Factor ZafA Regulates the Homeostatic and Adaptive Response to Zinc Starvation in Aspergillus Fumigatus. Genes, 9.","DOI":"10.3390\/genes9070318"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1080\/00362178585380481","article-title":"The Ecology of Paracoccidioides Brasiliensis: A Puzzle Still Unsolved","volume":"23","author":"Restrepo","year":"1985","journal-title":"Med. Mycol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.2217\/fmb-2020-0156","article-title":"Systemic Mycoses: A Potential Alert for Complications in COVID-19 Patients","volume":"15","author":"De","year":"2020","journal-title":"Future Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s11046-020-00462-9","article-title":"Fungal Co-Infections Associated with Global COVID-19 Pandemic: A Clinical and Diagnostic Perspective from China","volume":"185","author":"Song","year":"2020","journal-title":"Mycopathologia"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.1016\/j.micinf.2006.07.019","article-title":"Differential Gene Expression by Paracoccidioides Brasiliensis in Host Interaction Conditions: Representational Difference Analysis Identifies Candidate Genes Associated with Fungal Pathogenesis","volume":"8","author":"Schrank","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1111\/j.1574-695X.2007.00277.x","article-title":"The Transcriptional Profile of Paracoccidioides Brasiliensis Yeast Cells Is Influenced by Human Plasma","volume":"51","author":"Shrank","year":"2007","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3389\/fmicb.2011.00049","article-title":"The Homeostasis of Iron, Copper, and Zinc in Paracoccidioides Brasiliensis, Cryptococcus Neoformans Var. Grubii, and Cryptococcus Gattii: A Comparative Analysis","volume":"2","author":"Silva","year":"2011","journal-title":"Front. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.funbio.2013.04.004","article-title":"A Proteomic View of the Response of Paracoccidioides Yeast Cells to Zinc Deprivation","volume":"117","author":"Parente","year":"2013","journal-title":"Fungal Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"FSO232","DOI":"10.4155\/fsoa-2017-0044","article-title":"Identification of Membrane Proteome of Paracoccidioides Lutzii and Its Regulation by Zinc","volume":"3","author":"Silva","year":"2017","journal-title":"Future Sci. OA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1101\/gr.2722704","article-title":"Identification of Mammalian MicroRNA Host Genes and Transcription Units","volume":"14","author":"Rodriguez","year":"2004","journal-title":"Genome Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Schnitger, A.K.D., Machova, A., Mueller, R.U., Androulidaki, A., Schermer, B., Pasparakis, M., Kr\u00f6nke, M., and Papadopoulou, N. (2011). Listeria Monocytogenes Infection in Macrophages Induces Vacuolar-Dependent Host MiRNA Response. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027435"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5160","DOI":"10.1038\/s41598-022-09163-3","article-title":"MicroRNA-Mediated Post-Transcriptional Regulation of Pinus Pinaster Response and Resistance to Pinewood Nematode","volume":"12","author":"Modesto","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"16153","DOI":"10.1038\/nplants.2016.153","article-title":"Cotton Plants Export MicroRNAs to Inhibit Virulence Gene Expression in a Fungal Pathogen","volume":"2","author":"Zhang","year":"2016","journal-title":"Nat. Plants"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.3389\/fmicb.2018.03057","article-title":"Cell Wall Synthesis, Development of Hyphae and Metabolic Pathways Are Processes Potentially Regulated by MicroRNAs Produced between the Morphological Stages of Paracoccidioides Brasiliensis","volume":"9","author":"Paccez","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"104772","DOI":"10.1016\/j.micinf.2020.10.008","article-title":"MiRNAs Regulate Iron Homeostasis in Paracoccidioides Brasiliensis","volume":"23","author":"Oliveira","year":"2021","journal-title":"Microbes Infect."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1128\/jcm.12.2.279-281.1980","article-title":"Growth of Paracoccidioides Brasiliensis Yeast Phase in a Chemically Defined Culture Medium","volume":"12","author":"Restrepo","year":"1980","journal-title":"J. Clin. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.3389\/fmicb.2019.01301","article-title":"Drug Repurposing for Paracoccidioidomycosis through a Computational Chemogenomics Framework","volume":"10","author":"Neves","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/nar\/gkr688","article-title":"MiRDeep2 Accurately Identifies Known and Hundreds of Novel MicroRNA Genes in Seven Animal Clades","volume":"40","author":"MacKowiak","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1261\/rna.5248604","article-title":"Fast and Effective Prediction of MicroRNA\/Target Duplexes","volume":"10","author":"Rehmsmeier","year":"2004","journal-title":"RNA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1002\/jssc.201100238","article-title":"Detection and Expression Analysis of Recombinant Proteins in Plant-Derived Complex Mixtures Using NanoUPLC-MSE","volume":"34","author":"Murad","year":"2011","journal-title":"J. Sep. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.3389\/fmicb.2017.02308","article-title":"Differential Metabolism of a Two-Carbon Substrate by Members of the Paracoccidioides Genus","volume":"8","author":"Baeza","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1002\/pmic.200800562","article-title":"The Detection, Correlation, and Comparison of Peptide Precursor and Product Ions from Data Independent LC-MS with Data Dependant LC-MS\/MS","volume":"9","author":"Geromanos","year":"2009","journal-title":"Proteomics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1093\/bioinformatics\/btu627","article-title":"FungiFun2: A Comprehensive Online Resource for Systematic Analysis of Gene Lists from Fungal Species","volume":"31","author":"Priebe","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"D459","DOI":"10.1093\/nar\/gkx989","article-title":"MetalPDB in 2018: A Database of Metal Sites in Biological Macromolecular Structures","volume":"46","author":"Putignano","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"D988","DOI":"10.1093\/nar\/gkab1049","article-title":"Ensembl 2022","volume":"50","author":"Cunningham","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast Universal RNA-Seq Aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: A Flexible Suite of Utilities for Comparing Genomic Features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"EdgeR: A Bioconductor Package for Differential Expression Analysis of Digital Gene Expression Data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_59","unstructured":"R Core Team, R. (2021). A Language and Environment for Statistical Computing, R Foundation for Statistical Computing."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1590\/1678-4685-gmb-2018-0014","article-title":"In Silico Characterization of MicroRNAs-like Sequences in the Genome of Paracoccidioides Brasiliensis","volume":"42","author":"Batista","year":"2019","journal-title":"Genet. Mol. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1038\/sj.emboj.7600137","article-title":"Bsd2 Binds the Ubiquitin Ligase Rsp5 and Mediates the Ubiquitination of Transmembrane Proteins","volume":"23","author":"Hettema","year":"2004","journal-title":"EMBO J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"7893","DOI":"10.1128\/MCB.20.21.7893-7902.2000","article-title":"Saccharomyces Cerevisiae Expresses Three Functionally Distinct Homologues of the Nramp Family of Metal Transporters","volume":"20","author":"Portnoy","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1039\/C4MT00331D","article-title":"An Evolutionary Perspective on Zinc Uptake by Human Fungal Pathogens","volume":"7","author":"Wilson","year":"2015","journal-title":"Metallomics"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"8520","DOI":"10.1074\/jbc.M116.762948","article-title":"Zinc Starvation Induces Autophagy in Yeast","volume":"292","author":"Kawamata","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_65","first-page":"711","article-title":"Insights Into Histoplasma Capsulatum Behavior on Zinc Deprivation","volume":"10","author":"Moraes","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1039\/C8MT00269J","article-title":"The Cellular Economy of the Saccharomyces Cerevisiae Zinc Proteome","volume":"10","author":"Wang","year":"2018","journal-title":"Metallomics"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.mycres.2008.03.004","article-title":"Oxidative Stress-Associated Impairment of Glucose and Ammonia Metabolism in the Filamentous Fungus, Aspergillus Niger B1-D","volume":"112","author":"Li","year":"2008","journal-title":"Mycol. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.bbamcr.2006.05.003","article-title":"Activation of Superoxide Dismutases: Putting the Metal to the Pedal","volume":"1763","author":"Culotta","year":"2006","journal-title":"Biochim. Biophys. Acta BBA-Mol. Cell Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5726","DOI":"10.1038\/sj.emboj.7601453","article-title":"Repression of ADH1 and ADH3 during Zinc Deficiency by Zap1-Induced Intergenic RNA Transcripts","volume":"25","author":"Bird","year":"2006","journal-title":"EMBO J."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Amini, S., Jacobsen, A., Ivanova, O., Lijnzaad, P., Heringa, J., Holstege, F.C.P., Feenstra, K.A., and Kemmeren, P. (2019). The Ability of Transcription Factors to Differentially Regulate Gene Expression Is a Crucial Component of the Mechanism Underlying Inversion, a Frequently Observed Genetic Interaction Pattern. PLoS Comput. Biol., 15.","DOI":"10.1371\/journal.pcbi.1007061"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Nicola, A.M., Andrade, R.V., Dantas, A.S., Andrade, P.A., Arraes, F.B.M., Fernandes, L., Silva-Pereira, I., and Felipe, M.S.S. (2008). The Stress Responsive and Morphologically Regulated Hsp90 Gene from Paracoccidioides Brasiliensis Is Essential to Cell Viability. BMC Microbiol., 8.","DOI":"10.1186\/1471-2180-8-158"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.fgb.2012.11.005","article-title":"Involvement of the 90 KDa Heat Shock Protein during Adaptation of Paracoccidioides Brasiliensis to Different Environmental Conditions","volume":"51","author":"Tamayo","year":"2013","journal-title":"Fungal Genet. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nrm.2017.20","article-title":"The HSP90 Chaperone Machinery","volume":"18","author":"Schopf","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1074\/jbc.REV118.002806","article-title":"Hsp90 and Hsp70 Chaperones: Collaborators in Protein Remodeling","volume":"294","author":"Genest","year":"2019","journal-title":"J. Biol. Chem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/msb.2011.48","article-title":"A Dynamic Model of Proteome Changes Reveals New Roles for Transcript Alteration in Yeast","volume":"7","author":"Lee","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1038\/s42003-021-02142-w","article-title":"A Combined Approach for Single-Cell MRNA and Intracellular Protein Expression Analysis","volume":"4","author":"Tarbier","year":"2021","journal-title":"Commun. Biol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"14492","DOI":"10.1038\/s41598-018-32792-6","article-title":"Specificity of the Osmotic Stress Response in Candida Albicans Highlighted by Quantitative Proteomics","volume":"8","author":"Jacobsen","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1091\/mbc.e05-06-0501","article-title":"Role of the Hog1 Stress-Activated Protein Kinase in the Global Transcriptional Response to Stress in the Fungal Pathogen Candida Albicans","volume":"17","author":"Enjalbert","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"ref_79","first-page":"39","article-title":"Repression of the Acid ZrfA\/ZrfB Zinc-Uptake System of Aspergillus Fumigatus Mediated by PacC under Neutral, Zinc-Limiting Conditions","volume":"12","author":"Amich","year":"2009","journal-title":"Int. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1038\/sj.emboj.7600122","article-title":"The Zap1 Transcriptional Activator Also Acts as a Repressor by Binding Downstream of the TATA Box in ZRT2","volume":"23","author":"Bird","year":"2004","journal-title":"EMBO J."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s12281-012-0108-8","article-title":"Metal Acquisition and Homeostasis in Fungi","volume":"6","author":"Parente","year":"2012","journal-title":"Curr. Fungal Infect. Rep."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.tim.2018.10.011","article-title":"MicroRNAs at the Host-Bacteria Interface: Host Defense or Bacterial Offense","volume":"27","author":"Aguilar","year":"2019","journal-title":"Trends Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1186\/s13054-016-1555-3","article-title":"The Involvement of Regulatory Non-Coding RNAs in Sepsis: A Systematic Review","volume":"20","author":"Ho","year":"2016","journal-title":"Crit. Care"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s11046-014-9764-2","article-title":"Zinc Acquisition: A Key Aspect in Aspergillus Fumigatus Virulence","volume":"178","author":"Amich","year":"2014","journal-title":"Mycopathologia"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"5044","DOI":"10.1128\/MCB.17.9.5044","article-title":"Zap1p, a Metalloregulatory Protein Involved in Zinc-Responsive Transcriptional Regulation in Saccharomyces Cerevisiae","volume":"17","author":"Zhao","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Ferguson, J., Chang, J.T., and Kluger, Y. (2007). Inter- and Intra-Combinatorial Regulation by Transcription Factors and MicroRNAs. 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