{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:15:02Z","timestamp":1792091702395,"version":"4.4.0"},"reference-count":112,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca-Agencia de Ciencia y Tecnolog\u00eda de la Regi\u00f3n de Murcia, Spain","doi-asserted-by":"publisher","award":["21969\/PI\/22"],"award-info":[{"award-number":["21969\/PI\/22"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca-Agencia de Ciencia y Tecnolog\u00eda de la Regi\u00f3n de Murcia, Spain","doi-asserted-by":"publisher","award":["PID2021-124674NB-I00"],"award-info":[{"award-number":["PID2021-124674NB-I00"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"European Union","doi-asserted-by":"publisher","award":["21969\/PI\/22"],"award-info":[{"award-number":["21969\/PI\/22"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"European Union","doi-asserted-by":"publisher","award":["PID2021-124674NB-I00"],"award-info":[{"award-number":["PID2021-124674NB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The classification of Mucorales encompasses a collection of basal fungi that have traditionally demonstrated an aversion to modern genetic manipulation techniques. This aversion led to a scarcity of knowledge regarding their biology compared to other fungal groups. However, the emergence of mucormycosis, a fungal disease caused by Mucorales, has attracted the attention of the clinical field, mainly because available therapies are ineffective for decreasing the fatal outcome associated with the disease. This revitalized curiosity about Mucorales and mucormycosis, also encouraged by the recent COVID-19 pandemic, has spurred a significant and productive effort to uncover their mysteries in recent years. Here, we elaborate on the most remarkable breakthroughs related to the recently discovered genetic advances in Mucorales and mucormycosis. The utilization of a few genetic study models has enabled the identification of virulence factors in Mucorales that were previously described in other pathogens. More notably, recent investigations have identified novel genes and mechanisms controlling the pathogenic potential of Mucorales and their interactions with the host, providing fresh avenues to devise new strategies against mucormycosis. Finally, new study models are allowing virulence studies that were previously hampered in Mucorales, predicting a prolific future for the field.<\/jats:p>","DOI":"10.3390\/jof9030335","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T02:01:47Z","timestamp":1678327307000},"page":"335","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Mucorales and Mucormycosis: Recent Insights and Future Prospects"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5406-1613","authenticated-orcid":false,"given":"Ghizlane","family":"Tahiri","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2059-303X","authenticated-orcid":false,"given":"Carlos","family":"Lax","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6608-4145","authenticated-orcid":false,"given":"Jos\u00e9 Tom\u00e1s","family":"C\u00e1novas-M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Carrillo-Mar\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Sanchis","sequence":"additional","affiliation":[{"name":"Serra H\u00fanter Fellow, Facultad de Medicina y Ciencias de la Salud, Departamento de Ciencias M\u00e9dicas B\u00e1sicas, Unidad de Microbiolog\u00eda y Microbiolog\u00eda Ambiental, Universidad Rovira i Virgili, 43003 Reus, Spain"},{"name":"Institut d\u2019Investigaci\u00f3 Sanit\u00e0ria Pere Virgili (IISPV), 43003 Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3091-4378","authenticated-orcid":false,"given":"Eusebio","family":"Navarro","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7605-1726","authenticated-orcid":false,"given":"Victoriano","family":"Garre","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3762-5971","authenticated-orcid":false,"given":"Francisco Esteban","family":"Nicol\u00e1s","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.ijantimicag.2017.08.010","article-title":"Antifungal resistance in Mucorales","volume":"50","author":"Dannaoui","year":"2017","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S23","DOI":"10.1093\/cid\/cir866","article-title":"Epidemiology and Clinical Manifestations of Mucormycosis","volume":"54","author":"Petrikkos","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Carnero, L.C., and Mora-Montes, H.M. (2022). Mucormycosis and COVID-19-Associated Mucormycosis: Insights of a Deadly but Neglected Mycosis. J. Fungi, 8.","DOI":"10.3390\/jof8050445"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cmi.2018.07.011","article-title":"The epidemiology and clinical manifestations of mucormycosis: A systematic review and meta-analysis of case reports","volume":"25","author":"Jeong","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S245","DOI":"10.1093\/mmy\/myz011","article-title":"Pathogenicity patterns of mucormycosis: Epidemiology, interaction with immune cells and virulence factors","volume":"57","author":"Hassan","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"402","DOI":"10.2310\/7070.2005.34607","article-title":"Mucormycosis in immunocompetent individuals: An increasing trend","volume":"34","author":"Sridhara","year":"2005","journal-title":"J. Otolaryngol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.mimet.2011.01.002","article-title":"High reliability transformation of the basal fungus Mucor circinelloides by electroporation","volume":"84","author":"Garre","year":"2011","journal-title":"J. Microbiol. Methods"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.mimet.2014.05.016","article-title":"Efficient transformation of Rhizopus delemar by electroporation of germinated spores","volume":"103","author":"Xu","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100124","DOI":"10.1016\/j.crmeth.2021.100124","article-title":"Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus Rhizopus microsporus","volume":"1","author":"Lax","year":"2021","journal-title":"Cell Rep. Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nature13575","article-title":"Antifungal drug resistance evoked via RNAi-dependent epimutations","volume":"513","author":"Calo","year":"2014","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1099\/jmm.0.46386-0","article-title":"Natural resistance, iron and infection: A challenge for clinical medicine","volume":"55","author":"Bullen","year":"2006","journal-title":"J. Med. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1172\/JCI82744","article-title":"Bicarbonate correction of ketoacidosis alters host-pathogen interactions and alleviates mucormycosis","volume":"126","author":"Gebremariam","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"10649","DOI":"10.1038\/s41598-022-14515-0","article-title":"Secretion of the siderophore rhizoferrin is regulated by the cAMP-PKA pathway and is involved in the virulence of Mucor lusitanicus","volume":"12","author":"Santoyo","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.biocel.2017.06.005","article-title":"The rhizoferrin biosynthetic gene in the fungal pathogen Rhizopus delemar is a novel member of the NIS gene family","volume":"89","author":"Carroll","year":"2017","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7660","DOI":"10.1038\/s41598-018-26051-x","article-title":"Components of a new gene family of ferroxidases involved in virulence are functionally specialized in fungal dimorphism","volume":"8","author":"Murcia","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1111\/j.1365-2958.2010.07234.x","article-title":"The high affinity iron permease is a key virulence factor required for Rhizopus oryzae pathogenesis","volume":"77","author":"Ibrahim","year":"2010","journal-title":"Mol. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Schwartze, V.U., Winter, S., Shelest, E., Marcet-Houben, M., Horn, F., Wehner, S., Linde, J., Valiante, V., Sammeth, M., and Riege, K. (2014). Gene Expansion Shapes Genome Architecture in the Human Pathogen Lichtheimia corymbifera: An Evolutionary Genomics Analysis in the Ancient Terrestrial Mucorales (Mucoromycotina). PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004496"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/21505594.2015.1009732","article-title":"Iron starvation induces apoptosis in Rhizopus oryzae in vitro","volume":"6","author":"Shirazi","year":"2015","journal-title":"Virulence"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15898","DOI":"10.1038\/s41598-017-16123-9","article-title":"Intrinsic short-tailed azole resistance in mucormycetes is due to an evolutionary conserved aminoacid substitution of the lanosterol 14\u03b1-demethylase","volume":"7","author":"Caramalho","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e03386-22","DOI":"10.1128\/mbio.03386-22","article-title":"cotH Genes Are Necessary for Normal Spore Formation and Virulence in Mucor lusitanicus","volume":"14","author":"Szebenyi","year":"2023","journal-title":"MBio"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"eaaw1327","DOI":"10.1126\/sciadv.aaw1327","article-title":"Anti-CotH3 antibodies protect mice from mucormycosis by prevention of invasion and augmenting opsonophagocytosis","volume":"5","author":"Gebremariam","year":"2019","journal-title":"Sci. Adv."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1080\/21505594.2018.1435249","article-title":"Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies","volume":"9","author":"Sanchis","year":"2018","journal-title":"Virulence"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Arques, C., Navarro-Mendoza, M.I., Murcia, L., Navarro, E., Garre, V., and Nicol\u00e1s, F.E. (2021). The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales. Int. J. Mol. Sci., 22.","DOI":"10.20944\/preprints202101.0277.v1"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e02765-18","DOI":"10.1128\/mBio.02765-18","article-title":"Mucor circinelloides Thrives inside the Phagosome through an Atf-Mediated Germination Pathway","volume":"10","author":"Murcia","year":"2019","journal-title":"Mbio"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Trieu, T.A., Navarro-Mendoza, M.I., Perez-Arques, C., Sanchis, M., Capilla, J., Navarro-Rodriguez, P., Lopez-Fernandez, L., Torres-Martinez, S., Garre, V., and Ruiz-Vazquez, R.M. (2017). RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006150"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Lee, S.C., Li, A., Calo, S., and Heitman, J. (2013). Calcineurin plays key roles in the dimorphic transition and virulence of the human pathogenic zygomycete Mucor circinelloides. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003625"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1111\/mmi.13071","article-title":"Calcineurin orchestrates dimorphic transitions, antifungal drug responses and host-pathogen interactions of the pathogenic mucoralean fungus Mucor circinelloides","volume":"97","author":"Lee","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1128\/EC.00026-09","article-title":"A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides","volume":"8","author":"Ocampo","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"ref_29","first-page":"e13236","article-title":"The heterotrimeric G-protein beta subunit Gpb1 controls hyphal growth under low oxygen conditions through the protein kinase A pathway and is essential for virulence in the fungus Mucor circinelloides","volume":"22","author":"Vellanki","year":"2020","journal-title":"Cell Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Arques, C., Navarro-Mendoza, M.I., Murcia, L., Navarro, E., Garre, V., and Nicol\u00e1s, F.E. (2020). A non-canonical RNAi pathway controls virulence and genome stability in Mucorales. PLoS Genet., 16.","DOI":"10.1101\/2020.01.14.906289"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1574-6968.1992.tb05285.x","article-title":"Rhizoferrin: A complexone type siderophore of the Mucorales and entomophthorales (Zygomycetes)","volume":"73","author":"Thieken","year":"1992","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/s00253-003-1335-2","article-title":"Molecular genetics of fungal siderophore biosynthesis and uptake: The role of siderophores in iron uptake and storage","volume":"62","author":"Haas","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Liu, M., Lin, L., Gebremariam, T., Luo, G., Skory, C.D., French, S.W., Chou, T.F., Edwards, J.E., Ibrahim, A.S., and Edwards, J.E. (2015). Fob1 and Fob2 Proteins Are Virulence Determinants of Rhizopus oryzae via Facilitating Iron Uptake from Ferrioxamine. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004842"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0891-5520(02)00068-5","article-title":"Clinical pharmacology of antifungal compounds","volume":"17","author":"Groll","year":"2003","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.2165\/11596540-000000000-00000","article-title":"Triazole antifungal agents in invasive fungal infections: A comparative review","volume":"71","year":"2011","journal-title":"Drugs"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/0006-291X(89)91792-0","article-title":"Defective sterol C5-6 desaturation and azole resistance: A new hypothesis for the mode of action of azole antifungals","volume":"164","author":"Watson","year":"1989","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s10096-006-0107-1","article-title":"Zygomycosis: The re-emerging fungal infection","volume":"25","author":"Chayakulkeeree","year":"2006","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/jac\/dkz400","article-title":"Molecular and genetic basis of azole antifungal resistance in the opportunistic pathogenic fungus Candida albicans","volume":"75","author":"Nishimoto","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"697","DOI":"10.2217\/fmb.14.27","article-title":"Exploring azole antifungal drug resistance in Aspergillus fumigatus with special reference to resistance mechanisms","volume":"9","author":"Chowdhary","year":"2014","journal-title":"Future Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1128\/JCM.06133-11","article-title":"Antifungal susceptibility and phylogeny of opportunistic members of the order Mucorales","volume":"50","author":"Vitale","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/j.funbio.2015.08.009","article-title":"Phylogenetic analysis of fungal heterotrimeric G protein-encoding genes and their expression during dimorphism in Mucor circinelloides","volume":"119","year":"2015","journal-title":"Fungal. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3340","DOI":"10.1128\/AAC.00313-13","article-title":"Efficacy of liposomal amphotericin B and posaconazole in intratracheal models of murine mucormycosis","volume":"57","author":"Luo","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1128\/AAC.04424-14","article-title":"Susceptibility profiles of amphotericin B and posaconazole against clinically relevant Mucorales species under hypoxic conditions","volume":"59","author":"Maurer","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5420","DOI":"10.1128\/AAC.00905-16","article-title":"Aspergillus fumigatus Intrinsic Fluconazole Resistance Is Due to the Naturally Occurring T301I Substitution in Cyp51Ap","volume":"60","author":"Leonardelli","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1128\/AAC.01599-09","article-title":"Azole resistance profile of amino acid changes in Aspergillus fumigatus CYP51A based on protein homology modeling","volume":"54","author":"Snelders","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1128\/AAC.47.3.1120-1124.2003","article-title":"A point mutation in the 14\u03b1-sterol demethylase gene cyp51a contributes to itraconazole resistance in Aspergillus fumigatus","volume":"47","author":"Mellado","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e00536-15","DOI":"10.1128\/mBio.00536-15","article-title":"Genomic context of azole resistance mutations in Aspergillus fumigatus determined using whole-genome sequencing","volume":"6","author":"Abdolrasouli","year":"2015","journal-title":"MBio"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.3389\/fmicb.2016.01382","article-title":"Epidemiological and Genomic Landscape of Azole Resistance Mechanisms in Aspergillus Fungi","volume":"7","author":"Hagiwara","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"26213","DOI":"10.1038\/srep26213","article-title":"Triazole resistance mediated by mutations of a conserved active site tyrosine in fungal lanosterol 14\u03b1-demethylase","volume":"6","author":"Sagatova","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/nrmicro2921","article-title":"The Bacillus subtilis endospore: Assembly and functions of the multilayered coat","volume":"11","author":"McKenney","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"E3482","DOI":"10.1073\/pnas.1605917113","article-title":"Phosphorylation of spore coat proteins by a family of atypical protein kinases","volume":"113","author":"Nguyen","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Saggese, A., Scamardella, V., Sirec, T., Cangiano, G., Isticato, R., Pane, F., Amoresano, A., Ricca, E., and Baccigalupi, L. (2014). Antagonistic role of CotG and CotH on spore germination and coat formation in Bacillus subtilis. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104900"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"12218","DOI":"10.1038\/ncomms12218","article-title":"An integrated genomic and transcriptomic survey of mucormycosis-causing fungi","volume":"7","author":"Chibucos","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1172\/JCI71349","article-title":"CotH3 mediates fungal invasion of host cells during mucormycosis","volume":"124","author":"Gebremariam","year":"2014","journal-title":"J. Clin. Investig."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e01087-20","DOI":"10.1128\/mBio.01087-20","article-title":"GRP78 and Integrins Play Different Roles in Host Cell Invasion during Mucormycosis","volume":"11","author":"Alqarihi","year":"2020","journal-title":"MBio"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1016\/j.ygeno.2018.09.003","article-title":"Comparative analysis of five Mucor species transcriptomes","volume":"111","author":"Lebreton","year":"2019","journal-title":"Genomics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e00746-18","DOI":"10.1128\/JCM.00746-18","article-title":"PCR-based approach targeting Mucorales-specific gene family for diagnosis of mucormycosis","volume":"56","author":"Baldin","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1093\/femsre\/fuv035","article-title":"Fungal dimorphism: The switch from hyphae to yeast is a specialized morphogenetic adaptation allowing colonization of a host","volume":"39","author":"Boyce","year":"2015","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1586\/eri.11.152","article-title":"Candida albicans dimorphism as a therapeutic target","volume":"10","author":"Jacobsen","year":"2014","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_60","first-page":"203","article-title":"Identification and analysis of genes involved in the control of dimorphism in Mucor circinelloides (syn. racemosus)","volume":"2","author":"Wolff","year":"2002","journal-title":"FEMS Yeast Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1128\/mr.55.2.234-258.1991","article-title":"Mucor dimorphism","volume":"55","author":"Orlowski","year":"1991","journal-title":"Microbiol. Rev."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Chang, Z., Billmyre, R.B., Lee, S.C., and Heitman, J. (2019). Broad antifungal resistance mediated by RNAi-dependent epimutation in the basal human fungal pathogen Mucor circinelloides. PLoS Genet., 15.","DOI":"10.1101\/526459"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/bs.adgen.2015.07.002","article-title":"Distinct RNAi Pathways in the Regulation of Physiology and Development in the Fungus Mucor circinelloides","volume":"91","author":"Garre","year":"2015","journal-title":"Adv. Genet."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1365-2958.2011.07939.x","article-title":"Two distinct RNA-dependent RNA polymerases are required for initiation and amplification of RNA silencing in the basal fungus Mucor circinelloides","volume":"83","author":"Calo","year":"2012","journal-title":"Mol. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1128\/EC.00191-09","article-title":"A Single dicer Gene Is Required for Efficient Gene Silencing Associated with Two Classes of Small Antisense RNAs in Mucor circinelloides","volume":"8","author":"Calo","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Cervantes, M., Vila, A., Nicol\u00e1s, F.E., Moxon, S., de Haro, J.P., Dalmay, T., Torres-Mart\u00ednez, S., and Ruiz-V\u00e1zquez, R.M. (2013). A Single Argonaute Gene Participates in Exogenous and Endogenous RNAi and Controls Cellular Functions in the Basal Fungus Mucor circinelloides. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0069283"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"5535","DOI":"10.1093\/nar\/gkq301","article-title":"Endogenous short RNAs generated by Dicer 2 and RNA-dependent RNA polymerase 1 regulate mRNAs in the basal fungus Mucor circinelloides","volume":"38","author":"Nicolas","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Nicol\u00e1s, F.E., Vila, A., Moxon, S., Cascales, M.D., Torres-Mart\u00ednez, S., Ruiz-V\u00e1zquez, R.M., and Garre, V. (2015). The RNAi machinery controls distinct responses to environmental signals in the basal fungus Mucor circinelloides. BMC Genom., 16.","DOI":"10.1186\/s12864-015-1443-2"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Calo, S., Nicol\u00e1s, F.E., Lee, S.C., Vila, A., Cervantes, M., Torres-Martinez, S., Ruiz-Vazquez, R.M., Cardenas, M.E., and Heitman, J. (2017). A non-canonical RNA degradation pathway suppresses RNAi-dependent epimutations in the human fungal pathogen Mucor circinelloides. PLoS Genet., 13.","DOI":"10.1371\/journal.pgen.1006686"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Trieu, T.A., Calo, S., Nicol\u00e1s, F.E., Vila, A., Moxon, S., Dalmay, T., Torres-Mart\u00ednez, S., Garre, V., and Ruiz-V\u00e1zquez, R.M. (2015). A Non-canonical RNA Silencing Pathway Promotes mRNA Degradation in Basal Fungi. PLoS Genet., 11.","DOI":"10.1371\/journal.pgen.1005168"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e02579-19","DOI":"10.1128\/mBio.02579-19","article-title":"Drug-resistant epimutants exhibit organ-specific stability and induction during murine infections caused by the human fungal pathogen Mucor circinelloides","volume":"10","author":"Chang","year":"2019","journal-title":"MBio"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"C\u00e1novas-M\u00e1rquez, J.T., Navarro-Mendoza, M.I., P\u00e9rez-Arques, C., Lax, C., Tahiri, G., P\u00e9rez-Ruiz, J.A., Lorenzo-Guti\u00e9rrez, D., Calo, S., L\u00f3pez-Garc\u00eda, S., and Navarro, E. (2021). Role of the non-canonical rnai pathway in the antifungal resistance and virulence of Mucorales. Genes, 12.","DOI":"10.20944\/preprints202103.0717.v1"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Nicol\u00e1s, F.E., Murcia, L., Navarro, E., Navarro-Mendoza, M.I., P\u00e9rez-Arques, C., and Garre, V. (2020). Mucorales species and macrophages. J. Fungi, 6.","DOI":"10.3390\/jof6020094"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5294","DOI":"10.1093\/nar\/gkab238","article-title":"A ribonuclease III involved in virulence of Mucorales fungi has evolved to cut exclusively single-stranded RNA","volume":"49","author":"Falk","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev-genet-110711-155618","article-title":"RNase III: Genetics and Function; Structure and Mechanism","volume":"47","author":"Court","year":"2013","journal-title":"Annu. Rev. Genet."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.cell.2015.12.019","article-title":"Structure of Human DROSHA","volume":"164","author":"Kwon","year":"2016","journal-title":"Cell"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/j.cell.2018.03.080","article-title":"Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate","volume":"173","author":"Liu","year":"2018","journal-title":"Cell"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1365-2958.2007.06032.x","article-title":"A stepwise model for double-stranded RNA processing by ribonuclease III","volume":"67","author":"Gan","year":"2008","journal-title":"Mol. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s12281-019-0337-1","article-title":"Mucormycosis: Pathogenesis and Pathology","volume":"13","author":"Challa","year":"2019","journal-title":"Curr. Fungal Infect. Rep."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Harpf, V., Rambach, G., Parth, N., Neurauter, M., Fleischer, V., Lackner, M., Lass-Fl\u00f6rl, C., W\u00fcrzner, R., and Speth, C. (2023). Complement, but Not Platelets, Plays a Pivotal Role in the Outcome of Mucormycosis In Vivo. J. Fungi, 9.","DOI":"10.3390\/jof9020162"},{"key":"ref_81","first-page":"1375","article-title":"A zebrafish larval model reveals early tissue-specific innate immune responses to Mucor circinelloides","volume":"8","author":"Voelz","year":"2015","journal-title":"Dis. Model. Mech."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1038\/s41437-019-0191-5","article-title":"The model organism diaspora","volume":"123","author":"Hodgkin","year":"2019","journal-title":"Hered"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/BF02907500","article-title":"High frequency transformation of Mucor with recombinant plasmid DNA","volume":"49","author":"Roncero","year":"1984","journal-title":"Carlsberg Res. Commun."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s004380100558","article-title":"A negative regulator of light-inducible carotenogenesis in Mucor circinelloides","volume":"266","author":"Navarro","year":"2001","journal-title":"Mol. Genet. Genom."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3983","DOI":"10.1093\/emboj\/cdg384","article-title":"Two classes of small antisense RNAs in fungal RNA silencing triggered by non-integrative transgenes","volume":"22","author":"Nicolas","year":"2003","journal-title":"EMBO J."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1016\/j.cub.2019.09.024","article-title":"Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres","volume":"29","author":"Panchal","year":"2019","journal-title":"Curr. Biol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1007\/BF02907499","article-title":"Enrichment method for the isolation of auxotrophic mutants of Mucor using the polyene antibiotic N-glycosyl-polifungin","volume":"49","author":"Roncero","year":"1984","journal-title":"Carlsberg Res. Commun."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/BF00280302","article-title":"Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene","volume":"230","author":"Anaya","year":"1991","journal-title":"Mol. Genom."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.fgb.2006.09.003","article-title":"Mutants defective in a Mucor circinelloides dicer-like gene are not compromised in siRNA silencing but display developmental defects","volume":"44","author":"Nicolas","year":"2007","journal-title":"Fungal Genet. Biol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.1574-6968.2007.01044.x","article-title":"A RING-finger photocarotenogenic repressor involved in asexual sporulation in Mucor circinelloides","volume":"280","author":"Nicolas","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s00253-007-1281-5","article-title":"Lycopene over-accumulation by disruption of the negative regulator gene crgA in Mucor circinelloides","volume":"78","author":"Navarro","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Arques, C., Navarro-Mendoza, M.I., Murcia, L., Lax, C., Sanchis, M., Capilla, J., Navarro, E., Garre, V., and Nicol\u00e1s, F.E. (2021). A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene Network during Host Interactions. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020459"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1111\/j.1365-2958.2006.05291.x","article-title":"Distinct white collar-1 genes control specific light responses in Mucor circinelloides","volume":"61","author":"Silva","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.fgb.2012.12.003","article-title":"A White Collar 1-like protein mediates opposite regulatory functions in Mucor circinelloides","volume":"52","author":"Navarro","year":"2013","journal-title":"Fungal Genet. Biol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1111\/j.1365-2958.2008.06470.x","article-title":"A RING-finger protein regulates carotenogenesis via proteolysis-independent ubiquitylation of a White Collar-1-like activator","volume":"70","author":"Silva","year":"2008","journal-title":"Mol. Microbiol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"15348","DOI":"10.3390\/ijms140815348","article-title":"Functional Diversity of RNAi-Associated sRNAs in Fungi","volume":"14","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jbiotec.2009.04.003","article-title":"Transcriptional activation increases RNA silencing efficiency and stability in the fungus Mucor circinelloides","volume":"142","year":"2009","journal-title":"J. Biotechnol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/978-1-4939-8742-9_13","article-title":"Molecular tools for carotenogenesis analysis in the mucoral Mucor circinelloides","volume":"Volume 1852","author":"Navarro","year":"2018","journal-title":"Methods in Molecular Biology"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Prakash, H., and Chakrabarti, A. (2019). Global epidemiology of mucormycosis. J. Fungi, 5.","DOI":"10.3390\/jof5010026"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1038\/nature03997","article-title":"Pathogenic fungus harbours endosymbiotic bacteria for toxin production","volume":"437","author":"Hertweck","year":"2005","journal-title":"Nature"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1038\/s41467-017-02052-8","article-title":"Bacterial endosymbionts influence host sexuality and reveal reproductive genes of early divergent fungi","volume":"8","author":"Mondo","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.cub.2022.01.028","article-title":"A bacterial endosymbiont of the fungus Rhizopus microsporus drives phagocyte evasion and opportunistic virulence","volume":"32","author":"Itabangi","year":"2022","journal-title":"Curr. Biol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"101237","DOI":"10.1016\/j.xpro.2022.101237","article-title":"Transformation and CRISPR-Cas9-Mediated homologous recombination in the fungus Rhizopus microsporus","volume":"3","author":"Lax","year":"2022","journal-title":"Star. Protoc."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Ma, L.J., Ibrahim, A.S., Skory, C., Grabherr, M.G., Burger, G., Butler, M., Elias, M., Idnurm, A., Lang, B.F., and Sone, T. (2009). Genomic analysis of the basal lineage fungus Rhizopus oryzae reveals a whole-genome duplication. PLoS Genet., 5.","DOI":"10.1371\/journal.pgen.1000549"},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Lax, C., P\u00e9rez-arques, C., Navarro-mendoza, M.I., C\u00e1novas-m\u00e1rquez, J.T., Tahiri, G., P\u00e9rez-ruiz, J.A., Osorio-concepci\u00f3n, M., Murcia-flores, L., Navarro, E., and Garre, V. (2020). Genes, pathways, and mechanisms involved in the virulence of Mucorales. Genes, 11.","DOI":"10.3390\/genes11030317"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1111\/1469-0691.12466","article-title":"Why is mucormycosis more difficult to cure than more common mycoses?","volume":"20","author":"Katragkou","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1016\/j.cub.2016.04.038","article-title":"Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication","volume":"26","author":"Corrochano","year":"2016","journal-title":"Curr. Biol."},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Binder, U., Navarro-Mendoza, M.I., Naschberger, V., Bauer, I., Nicolas, F.E., Pallua, J.D., Lass-Fl\u00f6rl, C., and Garre, V. (2018). Generation of a mucor circinelloides reporter strain\u2014A promising new tool to study antifungal drug efficacy and mucormycosis. Genes, 9.","DOI":"10.3390\/genes9120613"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"12802","DOI":"10.1038\/s41598-018-30754-6","article-title":"An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages","volume":"8","author":"Garre","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Lax, C., C\u00e1novas-M\u00e1rquez, J.T., Tahiri, G., Navarro, E., Garre, V., and Nicol\u00e1s, F.E. (2022). Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis. Int. J. Mol Sci., 23.","DOI":"10.3390\/ijms23073454"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"e53","DOI":"10.1002\/cpmc.53","article-title":"Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation","volume":"49","author":"Vellanki","year":"2018","journal-title":"Curr. Protoc. Microbiol."},{"key":"ref_112","doi-asserted-by":"crossref","unstructured":"Osorio-Concepci\u00f3n, M., Lax, C., Navarro, E., Nicol\u00e1s, F.E., and Garre, V. (2021). DNA Methylation on N6-Adenine Regulates the Hyphal Development during Dimorphism in the Early-Diverging Fungus Mucor lusitanicus. J. 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