{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:18:01Z","timestamp":1790626681287,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T00:00:00Z","timestamp":1683936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hungarian National Research, Development and Innovation Fund","award":["NN 128867"],"award-info":[{"award-number":["NN 128867"]}]},{"name":"Hungarian National Research, Development and Innovation Fund","award":["K 138489"],"award-info":[{"award-number":["K 138489"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Alternative oxidase is a terminal oxidase in the branched mitochondrial electron transport chain of most fungi including Aspergillus niger (subgenus Circumdati, section Nigri). A second, paralogous aox gene (aoxB) is extant in some A. niger isolates but also present in two divergent species of the subgenus Nidulantes\u2014A. calidoustus and A. implicatus\u2014as well as in Penicillium swiecickii. Black aspergilli are cosmopolitan opportunistic fungi that can cause diverse mycoses and acute aspergillosis in immunocompromised individuals. Amongst the approximately 75 genome-sequenced A. niger strains, aoxB features considerable sequence variation. Five mutations were identified that rationally affect transcription or function or terminally modify the gene product. One mutant allele that occurs in CBS 513.88 and A. niger neotype strain CBS 554.65 involves a chromosomal deletion that removes exon 1 and intron 1 from aoxB. Another aoxB allele results from retrotransposon integration. Three other alleles result from point mutations: a missense mutation of the start codon, a frameshift, and a nonsense mutation. A. niger strain ATCC 1015 has a full-length aoxB gene. The A. niger sensu stricto complex can thus be subdivided into six taxa according to extant aoxB allele, which may facilitate rapid and accurate identification of individual species<\/jats:p>","DOI":"10.3390\/jof9050570","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T08:33:01Z","timestamp":1684139581000},"page":"570","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mutations in the Second Alternative Oxidase Gene: A New Approach to Group Aspergillus niger Strains"],"prefix":"10.3390","volume":"9","author":[{"given":"Michel","family":"Flipphi","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"M\u00e1rton","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"Juh\u00e1sz-Nagy P\u00e1l Doctoral School of Biology and Environmental Sciences, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivien","family":"B\u00edr\u00f3","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"Juh\u00e1sz-Nagy P\u00e1l Doctoral School of Biology and Environmental Sciences, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"\u00c1g","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8356-1716","authenticated-orcid":false,"given":"Erzs\u00e9bet","family":"S\u00e1ndor","sequence":"additional","affiliation":[{"name":"Institute of Food Science, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1854-9029","authenticated-orcid":false,"given":"Erzs\u00e9bet","family":"Fekete","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3077-8732","authenticated-orcid":false,"given":"Levente","family":"Karaffa","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,13]]},"reference":[{"key":"ref_1","first-page":"374","article-title":"Production of gluconic acid by using some irradiated microorganisms","volume":"8","author":"Ahmed","year":"2015","journal-title":"J. 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