{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:24:18Z","timestamp":1791933858443,"version":"4.3.4"},"reference-count":42,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T00:00:00Z","timestamp":1685232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology I + D + I of Spain","award":["CGL2012-39523-C02-01"],"award-info":[{"award-number":["CGL2012-39523-C02-01"]}]},{"name":"Ministry of Science and Technology I + D + I of Spain","award":["CTM2017-89565-C2-1-P"],"award-info":[{"award-number":["CTM2017-89565-C2-1-P"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Aspergillus is a genus of saprophytic fungus widely distributed in the environment and associated with soil, decaying vegetation, or seeds. However, some species, such as A. fumigatus, are considered opportunistic pathogens in humans. Their conidia (asexual spores) and mycelia are associated with clinical diseases known as invasive aspergillosis (IA), mainly related to the respiratory tract, such as allergic asthma, allergic bronchopulmonary aspergillosis (ABPA), or hypersensitivity. However, they can also disseminate to other organs, particularly the central nervous system. Due to the dispersal mechanism of the conidia through the air, airborne fungal particle measurement should be used to prevent and control this mold. This study aims to measure the outdoor airborne concentration of Aspergillus conidia and the Asp f 1 allergen concentration in Bellaterra (Barcelona, Spain) during 2021 and 2022, and to compare their dynamics to improve the understanding of the biology of this genus and contribute to a better diagnosis, prevention, and therapeutic measures in the face of possible health problems. The results show that both particles were airborne nearly all year round, but their concentrations showed no correlation. Due to Asp f 1 not being present in the conidia itself but being detectable during their germination and in hyphal fragments, we report the relevance of the aero-immunological analysis as a methodology to detect the potential pathogenic hazard of this fungus.<\/jats:p>","DOI":"10.3390\/jof9060624","type":"journal-article","created":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T15:30:47Z","timestamp":1685287847000},"page":"624","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Aspergillus Conidia and Allergens in Outdoor Environment: A Health Hazard?"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2047-3337","authenticated-orcid":false,"given":"Concepci\u00f3n De","family":"De Linares","sequence":"first","affiliation":[{"name":"Department of Botany, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Spain"},{"name":"Institut de Ci\u00e8ncia i Tecnologia Ambientals (ICTA-UAB), Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rut","family":"Puigdemunt","sequence":"additional","affiliation":[{"name":"Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Spain"},{"name":"Institut de Ci\u00e8ncia i Tecnologia Ambientals (ICTA-UAB), Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordina","family":"Belmonte","sequence":"additional","affiliation":[{"name":"Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Spain"},{"name":"Institut de Ci\u00e8ncia i Tecnologia Ambientals (ICTA-UAB), Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15598","DOI":"10.3402\/tellusb.v64i0.15598","article-title":"Primary biological aerosol particles in the atmosphere: A review","volume":"64","author":"Huffman","year":"2012","journal-title":"Tellus B Chem. Phys. Meteorol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mandrioli, P., Caneva, G., and Sabbioni, C. (2003). Cultural Heritage and Aerobiology. Methods and Measurement Techniques for Biodeterioration Monitoring, Kluwer Academic Publishers.","DOI":"10.1007\/978-94-017-0185-3"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.copbio.2004.04.009","article-title":"Detection and enumeration of airborne biocontaminants","volume":"15","author":"Stetzenbach","year":"2004","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e23448","DOI":"10.1002\/jcla.23448","article-title":"Molecular allergen sensitization of Aspergillus fumigatus between allergic bronchopulmonary aspergillosis and A. fumigatus-sensitized asthma in Guangzhou, Southern China","volume":"34","author":"Luo","year":"2020","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1111\/j.1398-9995.2005.00895.x","article-title":"Standard skin prick testing and sensitization to inhalant allergens across Europe\u2014A survey from the GA2LEN network","volume":"60","author":"Heinzerling","year":"2005","journal-title":"Allergy"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"205","DOI":"10.4168\/aair.2015.7.3.205","article-title":"Mold allergens in respiratory allergy: From structure to therapy","volume":"7","author":"Twaroch","year":"2015","journal-title":"Allergy Asthma Immunol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3109\/1040841X.2012.754401","article-title":"Allergic bronchopulmonary mycosis due to fungi other than Aspergillus: A global overview","volume":"40","author":"Chowdhary","year":"2014","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1159\/000090276","article-title":"Fungal allergies: A yet unsolved problem","volume":"91","author":"Crameri","year":"2006","journal-title":"Allergy Asthma Mod. Soc. A Sci. Approach"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S207","DOI":"10.1080\/13693780400025179","article-title":"The invasive and saprophytic syndromes due to Aspergillus spp.","volume":"43","author":"Hope","year":"2005","journal-title":"Med. Mycol."},{"key":"ref_10","first-page":"239","article-title":"Molecular characterization of Aspergillus fumigatus allergens","volume":"42","author":"Banerjee","year":"2000","journal-title":"Indian J. Chest Dis. Allied Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s10453-010-9177-8","article-title":"Airborne Aspergillus and Penicillium in the atmosphere of Szczecin (Poland) (2004\u20132009)","volume":"27","year":"2011","journal-title":"Aerobiologia"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s10453-011-9229-8","article-title":"Aerobiological monitoring of Aspergillus\/Penicillium spores during the potato storage","volume":"28","year":"2012","journal-title":"Aerobiologia"},{"key":"ref_13","first-page":"167","article-title":"Determination of Allergen 1 in Poultry Farms Using the Enzyme Immunoassay","volume":"61","author":"Prester","year":"2010","journal-title":"Arch. Ind. Hyg. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Dananch\u00e9, C., Gustin, M.P., Cassier, P., Loeffert, S.T., Thibaudon, M., B\u00e9net, T., and Vanhems, P. (2017). Evaluation of hirst-type spore trap to monitor environmental fungal load in hospital. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0177263"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s10453-021-09696-6","article-title":"Spatial distribution of fungi from the analysis of aerobiological data with a gamma function","volume":"37","author":"Canela","year":"2021","journal-title":"Aerobiologia"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1744-7348.1952.tb00904.x","article-title":"An automatic volumetric spore-trap","volume":"39","author":"Hirst","year":"1952","journal-title":"Ann. Appl. Biol."},{"key":"ref_17","unstructured":"Gal\u00e1n, C., Cari\u00f1anos, P., Alc\u00e1zar, P., and Dom\u00ednguez, E. (2007). Manual de Calidad y Gesti\u00f3n de la Red Espa\u00f1ola de Aerobiolog\u00eda; [Quality Manual and Management Spanish Aerobiology Network], Servicio de Publicaciones de la Universidad de C\u00f3rdoba."},{"key":"ref_18","first-page":"385","article-title":"Pollen monitoring: Minimum requirements and reproducibility of analysis","volume":"30","author":"Smith","year":"2017","journal-title":"Aerobiologia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s10453-017-9496-0","article-title":"Recommended terminology for aerobiological studies","volume":"33","author":"Ariatti","year":"2017","journal-title":"Aerobiolog\u00eda"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.fbr.2011.07.002","article-title":"Airborne Aspergillus fumigatus conidia: A risk factor for aspergillosis","volume":"25","year":"2011","journal-title":"Fungal Biol. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"De Linares, C., Navarro, D., Puigdemunt, R., and Belmonte, J. (2022). Airborne Alt a 1 dynamic and its relationship with the airborne dynamics of Alternaria conidia and Pleosporales spores. J. Fungi, 8.","DOI":"10.3390\/jof8020125"},{"key":"ref_22","unstructured":"(2023, April 25). Indoor Biotechnologies. Available online: https:\/\/inbio.com\/elisa-2.0\/elisa-2.0-kits-mold."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s00484-008-0191-2","article-title":"The effects of meteorological factors on airborne fungal spore concentration in two areas differing in urbanization level","volume":"53","author":"Oliveira","year":"2009","journal-title":"Int. J. Biometeorol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.1128\/aem.59.8.2648-2652.1993","article-title":"Abundance of airborne Penicillium CFU in relation to urbanization in Mexico City","volume":"59","author":"Rosas","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s10453-019-09569-z","article-title":"Analysis of the airborne fungal spores present in the atmosphere of Salamanca (MW Spain): A preliminary survey","volume":"35","year":"2019","journal-title":"Aerobiologia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.1016\/j.atmosenv.2008.01.009","article-title":"Prevalence of culturable airborne spores of selected allergenic and pathogenic fungi in outdoor air","volume":"42","author":"Fuller","year":"2008","journal-title":"Atmos. Environ."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.envpol.2005.07.004","article-title":"Correlation of ambient inhalable bioaerosols with particulate matter and ozone: A two-year study","volume":"140","author":"Adhikari","year":"2006","journal-title":"Environ. Pollut."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/00173134.1977.11864648","article-title":"A study of airborne fungal spores with the aid of the FOA slit-sampler","volume":"16","year":"1977","journal-title":"Grana"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1080\/00173139109432011","article-title":"Seasonal variation of outdoor airborne viable microfungi in Copenhagen, Denmark","volume":"30","author":"Larsen","year":"1991","journal-title":"Grana"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/j.1365-2222.1993.tb00330.x","article-title":"Environmental exposure to Aspergillus fumigatus allergen (Asp f I)","volume":"23","author":"Sporik","year":"1993","journal-title":"Clin. Exp. Allergy"},{"key":"ref_31","unstructured":"Buss Cezar Fontana, M. (2009). Estudio Epidemiol\u00f3gico de Alergia a Hongos y Otros Neumoalergenos, en Estudiantes de Medicina de la Universitat Aut\u00f2noma de Barcelona, con Relaci\u00f3n a Los Niveles F\u00fangicos Ambientales, Universitat Aut\u00f2noma de Barcelona."},{"key":"ref_32","unstructured":"R\u00faa Giraldo, A.L. (2013). Aerobiolog\u00eda de las Esporas de Pleosporales en Ambientes intra y Extradomiciliarios de Barcelona: Aplicaci\u00f3n a la Cl\u00ednica en Poblaci\u00f3n Al\u00e9rgica, Universitat Aut\u00f2noma de Barcelona."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"754","DOI":"10.3109\/02770903.2010.492539","article-title":"Quantification of airborne Aspergillus allergens: Redefining the approach","volume":"47","author":"Vermani","year":"2010","journal-title":"J. Asthma"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s10453-021-09721-8","article-title":"Aspergillus and Penicillium spores as urban pathogens of the Havana atmosphere, Cuba","volume":"37","author":"Almaguer","year":"2021","journal-title":"Aerobiologia"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s10453-005-4180-1","article-title":"Long term trends in outdoor Aspergillus\/Penicillium spore concentrations in Derby, UK from 1970 to 2003 and a comparative study in 1994 and 1996 with the indoor air of two local houses","volume":"21","author":"Millington","year":"2005","journal-title":"Aerobiologia"},{"key":"ref_36","first-page":"154","article-title":"Correlation between Alt a 1 levels and clinical symptoms in Alternaria alternata-monosensitized patients","volume":"22","author":"Brito","year":"2012","journal-title":"J. Investig. Allergol. Clin. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.funbio.2020.02.005","article-title":"Particle size distribution of the major Alternaria alternata allergen, Alt a 1, derived from airborne spores and subspore fragments","volume":"124","author":"Grewling","year":"2020","journal-title":"Fungal Biol."},{"key":"ref_38","first-page":"349","article-title":"Outdoor environmental levels of Aspergillus spp.","volume":"44","author":"Guinea","year":"2006","journal-title":"Conidia Over A Wide Geogr. Area. Sabouraudia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.atmosenv.2017.11.038","article-title":"Comparison of fungal spores concentrations measured with wideband integrated bioaerosol sensor and Hirst methodology","volume":"175","author":"Lemonis","year":"2018","journal-title":"Atmos. Environ."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/s10453-016-9436-4","article-title":"Comparisons of fungal spore distributions using air sampling at Worcester, England (2006\u20132010)","volume":"32","author":"Herbert","year":"2016","journal-title":"Aerobiologia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s10453-015-9417-z","article-title":"Determination of airborne fungal spores of Gaziantep (SE Turkey)","volume":"32","author":"Akata","year":"2016","journal-title":"Aerobiologia"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.scitotenv.2013.07.104","article-title":"Size-selective assessment of agricultural workers\u2019 personal exposure to airborne fungi and fungal fragments","volume":"466","author":"Lee","year":"2014","journal-title":"Sci. Total Environ."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/9\/6\/624\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:43:56Z","timestamp":1760125436000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/9\/6\/624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,28]]},"references-count":42,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["jof9060624"],"URL":"https:\/\/doi.org\/10.3390\/jof9060624","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,28]]}}}