{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:04:01Z","timestamp":1785801841699,"version":"3.56.0"},"reference-count":58,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation (FAPESP)","doi-asserted-by":"publisher","award":["2017\/00231-3"],"award-info":[{"award-number":["2017\/00231-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["2017\/00231-3"],"award-info":[{"award-number":["2017\/00231-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The gold standard for diagnosing invasive candidiasis still relies on blood cultures, which are inefficient and time-consuming to analyze. We developed an in-house qPCR assay to identify the 5 major Candida species in 78 peripheral blood (PB) samples from ICU patients at risk of candidemia. Blood cultures and (1,3)-\u03b2-D-glucan (BDG) testing were performed concurrently to evaluate the performance of the qPCR. The qPCR was positive for DNA samples from all 20 patients with proven candidemia (positive PB cultures), showing complete concordance with Candida species identification in blood cultures, except for detection of dual candidemia in 4 patients, which was missed by blood cultures. Additionally, the qPCR detected Candida species in six DNA samples from patients with positive central venous catheters blood (CB) but negative PB cultures. BDG values were similarly high in these six samples and the ones with proven candidemia, strongly suggesting the diagnosis of a true candidemia episode despite the negative PB cultures. Samples from patients neither infected nor colonized yielded negative results in both the qPCR and BDG testing. Our qPCR assay was at least as sensitive as blood cultures, but with a shorter turnaround time. Furthermore, negative results from the qPCR provided strong evidence for the absence of candidemia caused by the five major Candida species.<\/jats:p>","DOI":"10.3390\/jof9060635","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T02:57:10Z","timestamp":1685501830000},"page":"635","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia"],"prefix":"10.3390","volume":"9","author":[{"given":"Gabriel N.","family":"Felix","sequence":"first","affiliation":[{"name":"Laboratory of Medical Mycology (LIM 53), Instituto de Medicina Tropical, Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1112-081X","authenticated-orcid":false,"given":"Vera L. T.","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Imunologia (LIM 48), Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9509-9248","authenticated-orcid":false,"given":"Afonso R.","family":"da Silva Junior","sequence":"additional","affiliation":[{"name":"Central Laboratory Division (LIM 03), Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello M. C.","family":"Magri","sequence":"additional","affiliation":[{"name":"Central Laboratory Division (LIM 03), Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-010, Brazil"},{"name":"Departamento de Mol\u00e9stias Infecciosas e Parasit\u00e1rias, Faculdade de Medicina, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Rossi","sequence":"additional","affiliation":[{"name":"Central Laboratory Division (LIM 03), Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5319-0421","authenticated-orcid":false,"given":"Odeli N. E.","family":"Sejas","sequence":"additional","affiliation":[{"name":"Cancer Institute of S\u00e3o Paulo State (ICESP), Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 01246-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0765-6654","authenticated-orcid":false,"given":"Edson","family":"Abdala","sequence":"additional","affiliation":[{"name":"Cancer Institute of S\u00e3o Paulo State (ICESP), Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 01246-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz M. S.","family":"Malbouisson","sequence":"additional","affiliation":[{"name":"Discipline of Anesthesiology, Faculdade de Medicina, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7282-5453","authenticated-orcid":false,"given":"Thais","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department, Hospital do Servidor P\u00fablico Estadual de S\u00e3o Paulo (IAMSPE), S\u00e3o Paulo 04029-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5469-3602","authenticated-orcid":false,"given":"Gil","family":"Benard","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Mycology (LIM 53), Instituto de Medicina Tropical, Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4322-312X","authenticated-orcid":false,"given":"Gilda M. B.","family":"Del Negro","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Mycology (LIM 53), Instituto de Medicina Tropical, Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo (HC-FMUSP), S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0178420","DOI":"10.1128\/JCM.01784-20","article-title":"Recognition of Diagnostic Gaps for Laboratory Diagnosis of Fungal Diseases: Expert Opinion from the Fungal Diagnostics Laboratories Consortium (FDLC)","volume":"59","author":"Zhang","year":"2021","journal-title":"J. Clin. 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