{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:41:11Z","timestamp":1760128871841,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"COLCIENCIAS","award":["221356933625","DVG154","2016\u20132017"],"award-info":[{"award-number":["221356933625","DVG154","2016\u20132017"]}]},{"name":"Fondo de Investigaciones de la Universidad del Rosario","award":["221356933625","DVG154","2016\u20132017"],"award-info":[{"award-number":["221356933625","DVG154","2016\u20132017"]}]},{"name":"Comit\u00e9 para el Desarrollo de la Investigaci\u00f3n (CODI) of the Universidad de Antioquia","award":["221356933625","DVG154","2016\u20132017"],"award-info":[{"award-number":["221356933625","DVG154","2016\u20132017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Histoplasmosis is a fungal infection caused by the thermally dimorphic fungus Histoplasma capsulatum. This infection causes significant morbidity and mortality in people living with HIV\/AIDS, especially in countries with limited resources. Currently used diagnostic tests rely on culture and serology but with some limitations. No molecular assays are commercially available and the results from different reports have been variable. We aimed to evaluate quantitative real-time PCR (qPCR) targeting three protein-coding genes of Histoplasma capsulatum (100-kDa, H and M antigens) for detection of this fungus in formalin-fixed paraffin-embedded (FFPE) samples from patients with proven histoplasmosis. The sensitivity of 100-kDa, H and M qPCR assays were 93.9%, 91% and 57%, respectively. The specificity of 100-kDa qPCR was 93% when compared against samples from patients with other mycoses and other infections, and 100% when samples from patients with non-infectious diseases were used as controls. Our findings demonstrate that real-time PCR assays targeting 100-kDa and H antigen showed the most reliable results and can be successfully used for diagnosing this mycosis when testing FFPE samples.<\/jats:p>","DOI":"10.3390\/jof9070700","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T05:28:02Z","timestamp":1687757282000},"page":"700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets"],"prefix":"10.3390","volume":"9","author":[{"given":"Luisa F.","family":"L\u00f3pez","sequence":"first","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn 050034, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8305-7755","authenticated-orcid":false,"given":"\u00c1ngela M.","family":"Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto Colombiano de Medicina Tropical, Universidad CES, Medell\u00edn 055450, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"C\u00e1ceres","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn 050034, Colombia"},{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Chiller","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia P.","family":"Litvintseva","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lalitha","family":"Gade","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7052-7938","authenticated-orcid":false,"given":"\u00c1ngel","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Basic and Applied Microbiology Research Group (MICROBA), School of Microbiology, Universidad de Antioquia, Medell\u00edn 050010, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 111221, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Adenis, A., Nacher, M., Hanf, M., Vantilcke, V., Boukhari, R., Blachet, D., Demar, M., Aznar, C., Carme, B., and Couppie, P. 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