{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:02:34Z","timestamp":1765486954509,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCTI\/CNPq (Minist\u00e9rio da Ci\u00eancia e Tecnologia\/Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)"},{"name":"FNDCT (Fundo Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)"},{"name":"FAPEG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s)"},{"name":"FAPESB (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado da Bahia)"},{"name":"CAPES (Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\/Finance Code)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Background: The treatment of paracoccidioidomycosis (PCM) is a challenge, and the discovery of new antifungal compounds is crucial. The phenacylideneoxindoles exhibited promising antifungal activity against Paracoccidioides spp., but their mode of action remains unknown. Methods: Through proteomic analysis, we investigated the effects of (E)-3-(2-oxo-2-phenylethylidene)indolin-2-one on P. brasiliensis. In addition, we investigated the metabolic alterations of P. brasiliensis in response to the compound. Furthermore, the effects of the compound on the membrane, ethanol production, and reactive oxygen species (ROS) production were verified. Results: We identified differentially regulated proteins that revealed significant metabolic reorganization, including an increase in ethanol production, suggesting the activation of alcoholic fermentation and alterations in the rigidity of fungal cell membrane with an increase of the ergosterol content and formation of ROS. Conclusions: These findings enhance our understanding of the mode of action and response of P. brasiliensis to the investigated promising antifungal compound, emphasizing its potential as a candidate for the treatment of PCM.<\/jats:p>","DOI":"10.3390\/jof9080854","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T10:09:33Z","timestamp":1692180573000},"page":"854","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic Profiling of Paracoccidioides brasiliensis in Response to Phenacylideneoxindol Derivative: Unveiling Molecular Targets and Pathways"],"prefix":"10.3390","volume":"9","author":[{"given":"L\u00edvia do Carmo","family":"Silva","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2094-6042","authenticated-orcid":false,"given":"Olivia Basso","family":"Rocha","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor Godinho","family":"Portis","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thaynara Gonzaga","family":"Santos","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5350-7313","authenticated-orcid":false,"given":"Kleber Santiago","family":"Freitas e Silva","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimundo Francisco dos","family":"Santos Filho","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Universidade Federal da Bahia, Salvador 40170-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8822-517X","authenticated-orcid":false,"given":"Silvio","family":"Cunha","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Universidade Federal da Bahia, Salvador 40170-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f4nio","family":"Alonso","sequence":"additional","affiliation":[{"name":"Institute of Physics, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5378-9840","authenticated-orcid":false,"given":"C\u00e9lia Maria de Almeida","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-8036","authenticated-orcid":false,"given":"Maristela","family":"Pereira","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1590\/0037-8682-0230-2017","article-title":"Brazilian Guidelines for the Clinical Management of Paracoccidioidomycosis","volume":"50","author":"Mendes","year":"2017","journal-title":"Rev. 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