{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:45:49Z","timestamp":1768016749500,"version":"3.49.0"},"reference-count":23,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CONACyT-Mexico","award":["166052"],"award-info":[{"award-number":["166052"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The ascomycete Histoplasma capsulatum is the causative agent of systemic respiratory mycosis histoplasmosis, which sometimes develops acute disseminated or chronic clinical forms, with the latter usually associated with granuloma formation. The present report shows differential histopathological changes in the pulmonary inflammatory response of mice infected intranasally with the mycelial morphotype of H. capsulatum strains with distinct genotypes, EH-46 and G-217B, classified as LAm A2 and NAm 2 phylogenetic species, respectively. Infected male BALB\/c mice were sacrificed at different postinfection times, and their serial lung sections were stained with periodic acid\u2013Schiff and analyzed via microscopy. In mice infected with the LAm A2 strain, the results showed progressive changes in the inflammatory infiltrate of the lung parenchyma during the first hours and days postinfection as well as granulomas with macrophages containing intracellular yeast cells, which prevailed at 14 and 21 days postinfection. Bronchiolar-associated lymphoid tissue was induced in mice infected with both strains, primarily in mice infected with the NAm 2 strain. Several lung sections from mice infected with the LAm A2 strain showed PAS-positive yeast cells aggregated in a perinuclear crown-like arrangement in macrophages from 3 h to 21 days postinfection. These findings highlight differences in the host pulmonary inflammatory response associated with distinct H. capsulatum species.<\/jats:p>","DOI":"10.3390\/jof9100974","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T08:24:44Z","timestamp":1695889484000},"page":"974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species"],"prefix":"10.3390","volume":"9","author":[{"given":"Evelyn","family":"Pulido-Camarillo","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Tisular, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Av. Universidad 3000, Circuito Escolar s\/n, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge H.","family":"Sahaza","sequence":"additional","affiliation":[{"name":"Unidad de Micolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nayla","family":"de Souza Pitangui","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular e Molecular, Faculdade de Medicina, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto 14049-900, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8059-0826","authenticated-orcid":false,"given":"Maria Jos\u00e9 S.","family":"Mendes-Giannini","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual Paulista (UNESP), Araraquara 14800-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M.","family":"Fusco-Almeida","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual Paulista (UNESP), Araraquara 14800-903, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-7922","authenticated-orcid":false,"given":"Armando","family":"P\u00e9rez-Torres","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Tisular, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Av. Universidad 3000, Circuito Escolar s\/n, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lucia","family":"Taylor","sequence":"additional","affiliation":[{"name":"Unidad de Micolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.cimid.2014.03.002","article-title":"Usefulness of the murine model to study the immune response against Histoplasma capsulatum infection","volume":"37","author":"Sahaza","year":"2014","journal-title":"Comp. Immunol. Microbiol. Infect. 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