{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T15:47:08Z","timestamp":1773244028310,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Doctoral Fund Project of Southwest University, China","award":["SWU7110300034"],"award-info":[{"award-number":["SWU7110300034"]}]},{"name":"the Doctoral Fund Project of Southwest University, China","award":["SWU-KR22013"],"award-info":[{"award-number":["SWU-KR22013"]}]},{"name":"the Fundamental Research Funds for the Central Universities, Southwest University, China","award":["SWU7110300034"],"award-info":[{"award-number":["SWU7110300034"]}]},{"name":"the Fundamental Research Funds for the Central Universities, Southwest University, China","award":["SWU-KR22013"],"award-info":[{"award-number":["SWU-KR22013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Beauveria bassiana (B. bassiana) is a broad-spectrum entomopathogenic fungus that can control pests in agriculture and forestry. In this study, encoding ecdysteroid uridine diphosphate glucosyltransferase gene (egt) was successfully screened in B. bassiana on the medium containing 500\u03bcg\/mL G418 sulfate solution through the protoplast transformation method. This enzyme has the function of 20E (20-hydroxyecdysone) inactivation, thus increasing the mortality of the early instar larvae infected with B. bassiana. In this study, we transformed B. bassiana with the egt gene, which deactivates 20-hydroxyecdysone, a key hormone in insect development. The results showed that transgenic B. bassiana killed more silkworms of the 2nd instar larvae than the wild-type with a shorter LT50 time, which was reduced by approximately 20% (day 1 of the 2nd instar silkworm infection of B. bassiana) and 26.4% (day 2 of the 2nd instar silkworm infection of B. bassiana) compared to the wild-type, and also showed a higher mortality number before molting. The transgenic B. bassiana had a higher coverage of the body surface of silkworms compared to the wild type on the 3rd instar. In summary, improving entomopathogenic fungi using biological methods such as genetic engineering is feasible.<\/jats:p>","DOI":"10.3390\/jof9100987","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T11:58:57Z","timestamp":1696420737000},"page":"987","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ecdysteroid UDP-Glucosyltransferase Expression in Beauveria bassiana Increases Its Pathogenicity against Early Instar Silkworm Larvae"],"prefix":"10.3390","volume":"9","author":[{"given":"Xueqin","family":"Mao","sequence":"first","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongxu","family":"Xing","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, \/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Die","family":"Liu","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoran","family":"Xu","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyu","family":"Hou","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Lin","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9263-2886","authenticated-orcid":false,"given":"Qingyou","family":"Xia","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Lin","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanwang","family":"Shen","sequence":"additional","affiliation":[{"name":"Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1111\/j.1365-2672.2004.02215.x","article-title":"Molecular monitoring and evaluation of the application of the insect-pathogenic fungus Beauveria bassiana in southeast China","volume":"96","author":"Wang","year":"2004","journal-title":"J. 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