{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:34:36Z","timestamp":1760150076920,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T00:00:00Z","timestamp":1697760000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department for Environment, Food &amp; Rural Affairs","award":["TH0157","TH3_1","TH32324"],"award-info":[{"award-number":["TH0157","TH3_1","TH32324"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>In England, Cryphonectria parasitica was detected for the first time in 2011 in a nursery and in 2016 in the wider environment. Surveys between 2017 and 2020 identified the disease at different sites in Berkshire, Buckinghamshire, Cornwall, Derbyshire, Devon, Dorset, London, West Sussex, and the island of Jersey, while the present study comprises the results of the 2020\u20132023 survey with findings in Derbyshire, Devon, Kent, Nottinghamshire, Herefordshire, Leicestershire, London, West Sussex, and the islands of Jersey and Guernsey. A total of 226 suspected samples were collected from 72 surveyed sites, as far north as Edinburgh and as far west as Plymouth (both of which were negative), and 112 samples tested positive by real-time PCR and isolation from 35 sites. The 112 isolates were tested for the vegetative compatibility group (VCG), mating type, and Cryphonectria hypovirus 1 (CHV1). Twelve VCGs were identified, with two of them (EU-5 and EU-22) being the first records in the UK. Both mating types were present (37% MAT-1 and 63% MAT-2), but only one mating type was present per site and VCG, and perithecia were never observed. Cryphonectria hypovirus 1 (CHV1), consistently subtype-I haplotype E-5, was detected in three isolates at a low concentration (5.9, 21.1, and 33.0 ng\/\u00b5L) from locations in London, Nottinghamshire, and Devon.<\/jats:p>","DOI":"10.3390\/jof9101036","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T11:53:56Z","timestamp":1697802836000},"page":"1036","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cryphonectria parasitica Detections in England, Jersey, and Guernsey during 2020\u20132023 Reveal Newly Affected Areas and Infections by the CHV1 Mycovirus"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1983-1886","authenticated-orcid":false,"given":"Pedro","family":"Romon-Ochoa","sequence":"first","affiliation":[{"name":"Forest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pankajini","family":"Samal","sequence":"additional","affiliation":[{"name":"Forest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Gorton","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Lewis","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8900-7860","authenticated-orcid":false,"given":"Ruth","family":"Chitty","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Eacock","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elzbieta","family":"Krzywinska","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Crampton","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-1433","authenticated-orcid":false,"given":"Ana","family":"P\u00e9rez-Sierra","sequence":"additional","affiliation":[{"name":"Forest Research, THDAS-Tree Health Diagnostics and Advisory Service, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mick","family":"Biddle","sequence":"additional","affiliation":[{"name":"Forest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben","family":"Jones","sequence":"additional","affiliation":[{"name":"Forestry Commission, 620 Bristol Business Park, Bristol BS16 1EJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Ward","sequence":"additional","affiliation":[{"name":"Forest Research, Plant Pathology Department, Alice Holt Research Station, Farnham, Surrey GU10 4LH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"EPPO (2005). 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