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ARSB","award":["CNPq\/PECI: 200536\/2022-2"],"award-info":[{"award-number":["CNPq\/PECI: 200536\/2022-2"]}]},{"name":"Fopesq\/Proppi-UFF and Rede Micologia RJ. ARSB","award":["PIBIC-CNPq-UFF"],"award-info":[{"award-number":["PIBIC-CNPq-UFF"]}]},{"name":"Fopesq\/Proppi-UFF and Rede Micologia RJ. ARSB","award":["FAPERJ E-26\/211.502\/2021"],"award-info":[{"award-number":["FAPERJ E-26\/211.502\/2021"]}]},{"name":"Fopesq\/Proppi-UFF and Rede Micologia RJ. ARSB","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Fopesq\/Proppi-UFF and Rede Micologia RJ. ARSB","award":["308986\/2022-9"],"award-info":[{"award-number":["308986\/2022-9"]}]},{"name":"Fopesq\/Proppi-UFF and Rede Micologia RJ. ARSB","award":["302722\/2022-0"],"award-info":[{"award-number":["302722\/2022-0"]}]},{"name":"RLDM","award":["CNPq\/PECI: 200536\/2022-2"],"award-info":[{"award-number":["CNPq\/PECI: 200536\/2022-2"]}]},{"name":"RLDM","award":["PIBIC-CNPq-UFF"],"award-info":[{"award-number":["PIBIC-CNPq-UFF"]}]},{"name":"RLDM","award":["FAPERJ E-26\/211.502\/2021"],"award-info":[{"award-number":["FAPERJ E-26\/211.502\/2021"]}]},{"name":"RLDM","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"RLDM","award":["308986\/2022-9"],"award-info":[{"award-number":["308986\/2022-9"]}]},{"name":"RLDM","award":["302722\/2022-0"],"award-info":[{"award-number":["302722\/2022-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Sporothrix brasiliensis is the most pathogenic species, responsible for the Brazilian cat-transmitted sporotrichosis hyperendemic. In this scenario, an investigation of the pathogen\u2013host interaction can provide relevant information for future treatment strategies. To this end, the invertebrate Galleria mellonella has proven to be a suitable alternative for evaluating the virulence of pathogenic fungi, since the insect immune system is similar to the mammalian innate immune response. The aim of this work was to investigate phenotypic and molecular aspects of the immune response of G. mellonella throughout the S. brasiliensis infection. Hemocyte density and the evolution of the fungal load were evaluated. In parallel, RT-qPCR expression analysis of genes encoding antimicrobial peptides (Gallerimycin and Galiomycin) and stress management genes (C7 Contig 15362 and C8 Contig 19101) was conducted. The fungal load and hemocyte densities increased simultaneously and proportionally to the deleterious morphological events and larvae mortality. Gallerimycin, C7 Contig 15362 and C8 Contig 19101 genes were positively regulated (p &lt; 0.05) at distinct moments of S. brasiliensis infection, characterizing a time-dependent and alternately modulated profile. Galiomycin gene expression remained unchanged. Our results contribute to the future proposal of potential alternative pathways for treating and consequently controlling S. brasiliensis zoonosis, a major public health issue in Latin America.<\/jats:p>","DOI":"10.3390\/jof9111053","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T03:33:51Z","timestamp":1698377631000},"page":"1053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sporothrix brasiliensis Infection Modulates Antimicrobial Peptides and Stress Management Gene Expression in the Invertebrate Biomodel Galleria mellonella"],"prefix":"10.3390","volume":"9","author":[{"given":"Nath\u00e1lia Faria","family":"Reis","sequence":"first","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrela Concei\u00e7\u00e3o Santos","family":"de Jesus","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0245-2687","authenticated-orcid":false,"given":"Lais Cavalcanti dos Santos Velasco","family":"de Souza","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Martins","family":"Alc\u00e2ntara","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Andrade de Castro","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone Cristina Pereira","family":"Brito","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia de Azambuja","family":"Penna","sequence":"additional","affiliation":[{"name":"Laboratory of Insect Biology, Federal Fluminense 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Cocci, Biomedical Institute, Fluminense Federal University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8955-3204","authenticated-orcid":false,"given":"Ricardo Luiz Dantas","family":"Machado","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-0595","authenticated-orcid":false,"given":"Hector M.","family":"Mora-Montes","sequence":"additional","affiliation":[{"name":"Department of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Guanajuato 36050, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0461-7521","authenticated-orcid":false,"given":"Andr\u00e9a Regina de Souza","family":"Baptista","sequence":"additional","affiliation":[{"name":"Center for Microorganisms\u2019 Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Federal Fluminense University, Niter\u00f3i 24020-141, RJ, Brazil"},{"name":"Rede Micologia RJ\u2014Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ), Rio de Janeiro 21941-630, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gremi\u00e3o, I.D.F., Miranda, L.H.M., Reis, E.G., Rodrigues, A.M., and Pereira, S.A. 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