{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:38:37Z","timestamp":1760150317582,"version":"build-2065373602"},"reference-count":67,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T00:00:00Z","timestamp":1699315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005285","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Estado de Goi\u00e1s (FAPEG)","doi-asserted-by":"publisher","award":["201810267000022","405717\/2021-0"],"award-info":[{"award-number":["201810267000022","405717\/2021-0"]}],"id":[{"id":"10.13039\/501100005285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","doi-asserted-by":"publisher","award":["201810267000022","405717\/2021-0"],"award-info":[{"award-number":["201810267000022","405717\/2021-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The fungal pathogen Paracoccidioides lutzii causes systemic mycosis Paracoccidioidomycosis (PCM), which presents a broad distribution in Latin America. Upon infection, the fungus undergoes a morphological transition to yeast cells and provokes an inflammatory granulomatous reaction with a high number of neutrophils in the lungs. In this work, we employed proteomic analysis to investigate the in vitro response of the fungus to the interaction with human neutrophils. Proteomic profiling of P. lutzii yeast cells harvested at 2 and 4 h post interaction with human polymorphonuclear cells allowed the identification of 505 proteins differentially accumulated. The data indicated that P. lutzii yeast cells underwent a shift in metabolism from glycolysis to Beta oxidation, increasing enzymes of the glyoxylate cycle and upregulating enzymes related to the detoxification of oxidative and heat shock stress. To our knowledge, this is the first study employing proteomic analysis in the investigation of the response of a member of the Paracoccidioides genus to the interaction with neutrophils.<\/jats:p>","DOI":"10.3390\/jof9111088","type":"journal-article","created":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T00:44:18Z","timestamp":1699317858000},"page":"1088","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Response of Paracoccidioides lutzii to the Interaction with Human Neutrophils"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1655-9576","authenticated-orcid":false,"given":"Lana O\u2019Hara Souza","family":"Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goiania 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian Cristiane","family":"Baeza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Micologia M\u00e9dica, Centro de Ci\u00eancias M\u00e9dicas e Farmac\u00eauticas, Universidade Estadual do Oeste do Paran\u00e1, Cascavel 85819-110, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurine Lacerda","family":"Pigosso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goiania 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5350-7313","authenticated-orcid":false,"given":"Kleber Santiago Freitas e","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goiania 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-8036","authenticated-orcid":false,"given":"Maristela","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goiania 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1513-3247","authenticated-orcid":false,"given":"Marcos Antonio Batista","family":"de Carvalho J\u00fanior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goiania 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lia Maria","family":"de Almeida Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goiania 74690-900, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/02681218780000021","article-title":"Host-Parasite Relationships in Paracoccidioidomycosis","volume":"25","author":"Franco","year":"1987","journal-title":"J. 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