{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:47:23Z","timestamp":1790704043953,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Colombia\u2019s Ministry of Science, Technology, and Innovation (MINCIENCIAS)","award":["122284468008"],"award-info":[{"award-number":["122284468008"]}]},{"name":"Colombia\u2019s Ministry of Science, Technology, and Innovation (MINCIENCIAS)","award":["844-2019"],"award-info":[{"award-number":["844-2019"]}]},{"name":"Colombia\u2019s Ministry of Science, Technology, and Innovation (MINCIENCIAS)","award":["453-2020"],"award-info":[{"award-number":["453-2020"]}]},{"name":"School of Medicine and Health Sciences (ABN122), Universidad del Rosario, Bogot\u00e1 D.C., Colombia","award":["122284468008"],"award-info":[{"award-number":["122284468008"]}]},{"name":"School of Medicine and Health Sciences (ABN122), Universidad del Rosario, Bogot\u00e1 D.C., Colombia","award":["844-2019"],"award-info":[{"award-number":["844-2019"]}]},{"name":"School of Medicine and Health Sciences (ABN122), Universidad del Rosario, Bogot\u00e1 D.C., Colombia","award":["453-2020"],"award-info":[{"award-number":["453-2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study evaluated the activity of the synthetic peptide, PNR20, against Candida albicans ATCC 10231 and a MDR Colombian clinical isolate of Candida auris. Perturbation of yeast cell surface was evaluated using scanning electron microscopy. Cell viability of Vero cells was determined to assess peptide toxicity. Additionally, survival, fungal burden, and histopathology of BALB\/c mice infected intravenously with each Candida species and treated with PNR20 were analyzed. Morphological alterations were identified in both species, demonstrating the antifungal effect of PNR20. In vitro, Vero cells\u2019 viability was not affected by PNR20. All mice infected with either C. albicans or C. auris and treated with PNR20 survived and had a significant reduction in the fungal burden in the kidney compared to the control group. The histopathological analysis in mice infected and treated with PNR20 showed more preserved tissues, without the presence of yeast, compared to the control groups. This work shows that the utilization of PNR20 is a promising therapeutic alternative against disseminated candidiasis.<\/jats:p>","DOI":"10.3390\/jof9121149","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T11:43:16Z","timestamp":1701171796000},"page":"1149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Therapeutic Use of the Antimicrobial Peptide PNR20 to Resolve Disseminated Candidiasis in a Murine Model"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2838-9612","authenticated-orcid":false,"given":"Jeisson","family":"Micelly-Moreno","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences, Universidad Colegio Mayor de Cundinamarca, Bogota 110311, Colombia"},{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3485-6934","authenticated-orcid":false,"given":"Adriana","family":"Barreto-Santamar\u00eda","sequence":"additional","affiliation":[{"name":"Receptor-Ligand Department, Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Bogota 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5331-5693","authenticated-orcid":false,"given":"Gabriela","family":"Ar\u00e9valo-Pinz\u00f3n","sequence":"additional","affiliation":[{"name":"Microbiology Department, Faculty of Sciences, Pontificia Universidad Javeriana, Carrera 7 #40\u201362, Bogota 110231, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7547-5172","authenticated-orcid":false,"given":"Carolina","family":"Firacative","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1924","authenticated-orcid":false,"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3029-118X","authenticated-orcid":false,"given":"Patricia","family":"Escand\u00f3n","sequence":"additional","affiliation":[{"name":"Microbiology Group, Instituto Nacional de Salud, Bogota 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-2500","authenticated-orcid":false,"given":"Manuel A.","family":"Patarroyo","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Department, Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Bogota 111321, Colombia"},{"name":"Microbiology Department, Faculty of Medicine, Universidad Nacional de Colombia, Bogota 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4495-7077","authenticated-orcid":false,"given":"Juli\u00e1n E.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogota 111221, Colombia"},{"name":"Public Health Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18026","DOI":"10.1038\/nrdp.2018.26","article-title":"Invasive candidiasis","volume":"4","author":"Pappas","year":"2018","journal-title":"Nat. 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