{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:29:36Z","timestamp":1767850176281,"version":"3.49.0"},"reference-count":106,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008679","name":"the Ministry of Science, Technology and Innovation of Colombia","doi-asserted-by":"publisher","award":["FCB-02-19 project"],"award-info":[{"award-number":["FCB-02-19 project"]}],"id":[{"id":"10.13039\/501100008679","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008679","name":"the Ministry of Science, Technology and Innovation of Colombia","doi-asserted-by":"publisher","award":["FCB-02-19"],"award-info":[{"award-number":["FCB-02-19"]}],"id":[{"id":"10.13039\/501100008679","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008679","name":"the Ministry of Science, Technology and Innovation of Colombia","doi-asserted-by":"publisher","award":["CVU:227919"],"award-info":[{"award-number":["CVU:227919"]}],"id":[{"id":"10.13039\/501100008679","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the University of C\u00f3rdoba, Monter\u00eda, Colombia","award":["FCB-02-19 project"],"award-info":[{"award-number":["FCB-02-19 project"]}]},{"name":"the University of C\u00f3rdoba, Monter\u00eda, Colombia","award":["FCB-02-19"],"award-info":[{"award-number":["FCB-02-19"]}]},{"name":"the University of C\u00f3rdoba, Monter\u00eda, Colombia","award":["CVU:227919"],"award-info":[{"award-number":["CVU:227919"]}]},{"name":"CONACyT","award":["FCB-02-19 project"],"award-info":[{"award-number":["FCB-02-19 project"]}]},{"name":"CONACyT","award":["FCB-02-19"],"award-info":[{"award-number":["FCB-02-19"]}]},{"name":"CONACyT","award":["CVU:227919"],"award-info":[{"award-number":["CVU:227919"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Candida tropicalis, an opportunistic pathogen, ranks among the primary culprits of invasive candidiasis, a condition notorious for its resistance to conventional antifungal drugs. The urgency to combat these drug-resistant infections has spurred the quest for novel therapeutic compounds, with a particular focus on those of natural origin. In this study, we set out to evaluate the impact of isoespintanol (ISO), a monoterpene derived from Oxandra xylopioides, on the transcriptome of C. tropicalis. Leveraging transcriptomics, our research aimed to unravel the intricate transcriptional changes induced by ISO within this pathogen. Our differential gene expression analysis unveiled 186 differentially expressed genes (DEGs) in response to ISO, with a striking 85% of these genes experiencing upregulation. These findings shed light on the multifaceted nature of ISO\u2019s influence on C. tropicalis, spanning a spectrum of physiological, structural, and metabolic adaptations. The upregulated DEGs predominantly pertained to crucial processes, including ergosterol biosynthesis, protein folding, response to DNA damage, cell wall integrity, mitochondrial activity modulation, and cellular responses to organic compounds. Simultaneously, 27 genes were observed to be repressed, affecting functions such as cytoplasmic translation, DNA damage checkpoints, membrane proteins, and metabolic pathways like trans-methylation, trans-sulfuration, and trans-propylamine. These results underscore the complexity of ISO\u2019s antifungal mechanism, suggesting that it targets multiple vital pathways within C. tropicalis. Such complexity potentially reduces the likelihood of the pathogen developing rapid resistance to ISO, making it an attractive candidate for further exploration as a therapeutic agent. In conclusion, our study provides a comprehensive overview of the transcriptional responses of C. tropicalis to ISO exposure. The identified molecular targets and pathways offer promising avenues for future research and the development of innovative antifungal therapies to combat infections caused by this pathogenic yeast.<\/jats:p>","DOI":"10.3390\/jof9121199","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T06:53:16Z","timestamp":1702623196000},"page":"1199","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transcriptional Reprogramming of Candida tropicalis in Response to Isoespintanol Treatment"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5056-4355","authenticated-orcid":false,"given":"Orfa In\u00e9s","family":"Contreras-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Biology Department, Faculty of Basic Sciences, University of C\u00f3rdoba, Monter\u00eda 230002, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Angulo-Ort\u00edz","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Basic Sciences, University of C\u00f3rdoba, Monter\u00eda 230002, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9235-3844","authenticated-orcid":false,"given":"Gilmar","family":"Santaf\u00e9-Pati\u00f1o","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Basic Sciences, University of C\u00f3rdoba, Monter\u00eda 230002, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6338-7930","authenticated-orcid":false,"given":"Katia","family":"Avi\u00f1a-Padilla","sequence":"additional","affiliation":[{"name":"Center for Research and Advanced Studies of the I.P.N. Unit Irapuato, Irapuato 36821, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Camila","family":"Velasco-Pareja","sequence":"additional","affiliation":[{"name":"Bacteriology Department, Faculty of Health Sciences, University of C\u00f3rdoba, Monter\u00eda 230002, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8081-4212","authenticated-orcid":false,"given":"Mar\u00eda Fernanda","family":"Yasnot","sequence":"additional","affiliation":[{"name":"Bacteriology Department, Faculty of Health Sciences, University of C\u00f3rdoba, Monter\u00eda 230002, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e01793","DOI":"10.1016\/j.idcr.2023.e01793","article-title":"Candida Chorioretinitis in Renal Transplant Recipient with Candidemia Related to Contaminated Organ Preservation Fluid: A Role for Dilated Fundus Examination in Its Management","volume":"32","author":"Schils","year":"2023","journal-title":"IDCases"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.jiac.2023.03.018","article-title":"The First Case of Clade I Candida auris Candidemia in a Patient with COVID-19 in Japan","volume":"29","author":"Ohashi","year":"2023","journal-title":"J. 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