{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:18:23Z","timestamp":1760059103189,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","award":["Fundect\u2014358\/2022; 900\/2022","CNPq\u2014314858\/2020-2","FINEP\u201401.23.0679.00"],"award-info":[{"award-number":["Fundect\u2014358\/2022; 900\/2022","CNPq\u2014314858\/2020-2","FINEP\u201401.23.0679.00"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["Fundect\u2014358\/2022; 900\/2022","CNPq\u2014314858\/2020-2","FINEP\u201401.23.0679.00"],"award-info":[{"award-number":["Fundect\u2014358\/2022; 900\/2022","CNPq\u2014314858\/2020-2","FINEP\u201401.23.0679.00"]}]},{"name":"Financiadora de Estudos e Projetos","award":["Fundect\u2014358\/2022; 900\/2022","CNPq\u2014314858\/2020-2","FINEP\u201401.23.0679.00"],"award-info":[{"award-number":["Fundect\u2014358\/2022; 900\/2022","CNPq\u2014314858\/2020-2","FINEP\u201401.23.0679.00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoX"],"abstract":"<jats:p>Aedes aegypti (Linnaeus, 1762) is Brazil\u2019s primary vector of epidemiologically significant arboviruses such as yellow fever, dengue, Zika, and chikungunya. Despite using conventional chemical control measures, this species has developed resistance to standard chemical insecticides, prompting the search for natural larvicidal compounds. Plant protease inhibitors offer an insecticidal alternative as the primary digestive enzymes in the midgut of Ae. aegypti are proteases (trypsin and chymotrypsin). Ae. aegypti larvae fed with ILTI, a Kunitz-type trypsin inhibitor derived from Inga laurina seeds, at concentrations between 0.03 mg of protein per mL (mgP\/mL) and 0.12 mgP\/mL, exhibited delayed larval development, with a lethal concentration (LC50) of 0.095 mgP mL\u22121 of ILTI for 50% of fourth-instar larvae (L4). The ex vivo assay indicated that ILTI effectively inhibited the activity of larval trypsin, which remained susceptible to the inhibitor. Additionally, molecular modelling and docking studies were conducted to predict the three-dimensional ILTI\/enzyme molecular complexes at the atomic level. Therefore, the results demonstrate that ILTI functions as a protease inhibitor in this species, presenting itself as a promising larvicidal tool in the control of Ae. aegypti.<\/jats:p>","DOI":"10.3390\/jox15030077","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T04:32:37Z","timestamp":1747888357000},"page":"77","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of a Proteinase Inhibitor from Inga laurina Seeds (ILTI) on Aedes aegypti Larval Development"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8233-0713","authenticated-orcid":false,"given":"Ana","family":"Jacobowski","sequence":"first","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8216-3038","authenticated-orcid":false,"given":"Welington","family":"Leite","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antolim Martinez","family":"J\u00fanior","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduarda","family":"Reis","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2687-9525","authenticated-orcid":false,"given":"Lorena","family":"Pires","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vit\u00f3ria","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5330-5452","authenticated-orcid":false,"given":"Layza","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9835-467X","authenticated-orcid":false,"given":"Eduardo","family":"Arruda","sequence":"additional","affiliation":[{"name":"TechnoAnlogical Chemistry Laboratory, Federal University of Grande Dourados, Dourados 79.825-070, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9546-0525","authenticated-orcid":false,"given":"Oct\u00e1vio","family":"Franco","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Postgraduate Program in Biotechnology, Don Bosco Catholic University, Campo Grande 79.117-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6676-5362","authenticated-orcid":false,"given":"Marlon","family":"Cardoso","sequence":"additional","affiliation":[{"name":"S-Inova Biotech, Postgraduate Program in Biotechnology, Don Bosco Catholic University, Campo Grande 79.117-900, MS, Brazil"},{"name":"Postgraduate Program in Environmental Sciences and Agricultural Sustainability, Don Bosco Catholic University, Campo Grande 79.117-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1115-4083","authenticated-orcid":false,"given":"Priscila","family":"Hiane","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6969-6307","authenticated-orcid":false,"given":"Maria","family":"Macedo","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Purification and Their Biological Functions, Federal University of Mato Grosso do Sul, Campo Grande 79.050-010, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"ref_1","unstructured":"(2024, August 05). 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