{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:29:08Z","timestamp":1790774948284,"version":"4.1.0"},"reference-count":192,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoX"],"abstract":"<jats:p>People are exposed to pesticides daily through food, drinking water, and the environment, both in urban and rural settings. These chemicals, while offering economic and agricultural benefits through pest control and increased productivity, may pose a growing risk to human health and ecosystem biodiversity. While the European regulatory framework offers a robust foundation for risk assessment, significant limitations persist, especially in addressing cumulative exposure, low-dose effects, and chemical mixtures. This review focuses on selected scientific and regulatory challenges by reviewing recent European Food Safety Authority (EFSA) conclusions, Organization for Economic Co-operation and Development (OECD) test guidelines updates, and current European legislative approaches. Particular attention is given to the regulation of endocrine-disrupting and reprotoxic substances, highlighting progress and remaining gaps in implementation. A brief mention will also be made of immuno-toxic substances, for which no specific hazard class has yet been established. Building on official reports and peer-reviewed literature, this review provides a structured evaluation of the scientific and regulatory landscape, including underexplored issues like the transition to animal-free toxicology and integration of biomonitoring with health data. The goal is to propose realistic, evidence-based improvements to current frameworks using integrated, interdisciplinary approaches that connect toxicology, policy, and implementation science. A shift to a holistic, systems-based, and precautionary paradigm is vital to address emerging challenges and ensure strong protection of health and environment, as well as supporting the needs of the agricultural sector.<\/jats:p>","DOI":"10.3390\/jox15050173","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:17:35Z","timestamp":1761056255000},"page":"173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Scientific and Regulatory Perspectives on Chemical Risk Assessment of Pesticides in the European Union"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8586-6836","authenticated-orcid":false,"given":"Fabio","family":"Buonsenso","sequence":"first","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, Sesto Fiorentino, 50019 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3390\/insects6010152","article-title":"Integrated pest management for sustainable intensification of agriculture in Asia and Africa","volume":"6","author":"Pretty","year":"2015","journal-title":"Insects"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.taap.2013.01.025","article-title":"Pesticides and human chronic diseases: Evidences, mechanisms, and perspectives","volume":"268","author":"Mostafalou","year":"2013","journal-title":"Toxicol. 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