{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T21:40:38Z","timestamp":1781127638862,"version":"3.54.1"},"reference-count":59,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Commission Project BIORIMA and SUNRISE","award":["760928"],"award-info":[{"award-number":["760928"]}]},{"name":"European Commission Project BIORIMA and SUNRISE","award":["101137324"],"award-info":[{"award-number":["101137324"]}]},{"name":"national funds via FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia I.P","award":["UID\/50017\/2025"],"award-info":[{"award-number":["UID\/50017\/2025"]}]},{"name":"national funds via FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia I.P","award":["LA\/P\/0094\/2020"],"award-info":[{"award-number":["LA\/P\/0094\/2020"]}]},{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"FCT, I.P. via a research contract under the Scientific Employment Stimulus-Individual Call","award":["2021.02867.CEECIND\/CP1659\/CT0004"],"award-info":[{"award-number":["2021.02867.CEECIND\/CP1659\/CT0004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoX"],"abstract":"<jats:p>Silver nanomaterials (Ag NMs) are widely used, including in consumer products, and they inevitably enter the environment, with the soil compartment acting as a major sink. However, most available toxicity data focus on the reference Ag NM300K and rely on standard tests, even though long(er)-term exposure tests are recognized as particularly important for assessing the risks to soil invertebrates. Hence, the aim of the present study was to investigate the toxicity of commercial Ag NPs (Ag-Sigma, NPs &lt; 150 nm) to the soil ecotoxicology model Folsomia candida (Collembola). Effects were assessed based on the standard OECD reproduction test (28 days) and beyond, with exposure prolonged for a second generation (56 days). Results showed that, based on the standard test (50% reproduction effect concentration\u2014EC50 = 988 mg Ag\/kg soil), the commercial Ag NPs were less toxic than the reference Ag NM300K and the ionic form AgNO3 (from literature). However, the toxicity dramatically increased (ca. 4 times) during the second-generation exposure (EC50(56d) = 234 mg Ag\/kg soil), surpassing the toxicity of Ag NM300K. The decrease in adults\u2019 size indicates that moulting might be affected. Overall, increased toxicity in prolonged exposure was not expected based on the available and standard test results, which highlights the importance of long(er)-term exposures to fully assess the risks of NMs to soil communities.<\/jats:p>","DOI":"10.3390\/jox15060210","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T08:30:17Z","timestamp":1765528217000},"page":"210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hazard Assessment of Ag Nanoparticles in Soil Invertebrates\u2014Strong Impact on the Longer-Term Exposure of Folsomia candida"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7537-2341","authenticated-orcid":false,"given":"Susana I. L.","family":"Gomes","sequence":"first","affiliation":[{"name":"Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2260-1224","authenticated-orcid":false,"given":"Janeck J.","family":"Scott-Fordsmand","sequence":"additional","affiliation":[{"name":"Department of Ecoscience, Aarhus University, C.F. M\u00f8llers Alle 4, DK-8000 Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8137-3295","authenticated-orcid":false,"given":"M\u00f3nica J. B.","family":"Amorim","sequence":"additional","affiliation":[{"name":"Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"ref_1","unstructured":"IMARC (2025, September 03). 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