{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:07:43Z","timestamp":1779959263965,"version":"3.53.1"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoX"],"abstract":"<jats:p>Background: Acetamiprid (ACMP) exposure mediates a variety of pathological complications, including testicular toxicity. Berberine (BBR) is a plant-derived alkaloid with potential pharmacological properties. This study sought to evaluate the ameliorative effects of BBR against ACMP-induced testicular toxicity. Methods: Male Wistar rats were divided into four groups: control, BBR-treated, ACMP-exposed, and BBR+ACMP co-treated, and were administered with BBR (150 mg\/kg b.wt) and ACMP (21.7 mg\/kg b.wt) for 21 days. Biochemical and FTIR analyses, RT-PCR, computational analyses, and histopathological examination were conducted to assess alterations in lipid and protein profiles, as well as apoptotic and structural changes. Results: ACMP exposure was associated with oxidative injury, functional alterations (stretching of -OH, -CH2, -NH, C=O, C-N, -COO-, -PO2\u2212), and compositional changes in proteins and lipids. Pre-treatment of BBR (2 h prior) was associated with attenuation of the functional and compositional alterations in proteins and lipids in co-treated rats. RT-PCR and computational analysis showed increased Bax and caspase-3 and decreased Bcl-2 mRNA expression, suggesting a potential modulation of ACMP-induced apoptosis by BBR. Histological examination showed that pre-treatment with BBR prevented ACMP-induced structural alterations, including cellular disorganization and alteration in seminiferous tubules. Conclusions: The study suggested that the BBR may exert ameliorative effects against ACMP-induced testicular toxicity by modulating lipid and protein changes and the anti-apoptotic pathway. Thus, BBR could be used as a potential ameliorative agent against oxidative stress. However, more mechanistic studies are needed for broader biological relevance and validity.<\/jats:p>","DOI":"10.3390\/jox16030095","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T08:00:36Z","timestamp":1779955236000},"page":"95","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ameliorative Effects of Berberine Against Acetamiprid-Induced Toxicity in the Testes of Rats: A Computational and Histological Insight"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6936-9223","authenticated-orcid":false,"given":"Jagjeet","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Zoology, Government College, Satnali, Mahendergarh 123024, Haryana, India"},{"name":"Department of Zoology, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7456-5405","authenticated-orcid":false,"given":"Annu","family":"Phogat","sequence":"additional","affiliation":[{"name":"Department of Zoology, Government College, Satnali, Mahendergarh 123024, Haryana, India"},{"name":"Department of Zoology, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reena","family":"Sheoran","sequence":"additional","affiliation":[{"name":"Department of Zoology, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Hasanpuri","sequence":"additional","affiliation":[{"name":"Department of Zoology, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2415-5563","authenticated-orcid":false,"given":"Manoj Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Zoology, Rao Pahlad Singh Degree College, Balana, Mahendergarh 123029, Haryana, India"},{"name":"Department of Genetics, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8909-9542","authenticated-orcid":false,"given":"Vinay","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Zoology, Maharshi Dayanand University, Rohtak 124001, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1007\/s10311-021-01353-1","article-title":"Toxicity of the Acetamiprid Insecticide for Mammals: A Review","volume":"20","author":"Phogat","year":"2022","journal-title":"Environ. 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