{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:17:14Z","timestamp":1780579034255,"version":"3.54.1"},"reference-count":80,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Centro Ricerche ed Infrastrutture Marine Avanzate in Calabria","award":["RIC20SGREC_F_ZUPO\u2014BluCaProd"],"award-info":[{"award-number":["RIC20SGREC_F_ZUPO\u2014BluCaProd"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoX"],"abstract":"<jats:p>Coastal ecosystems are increasingly threatened by human activities, highlighting the need for sensitive tools to assess environmental risk. An active biomonitoring approach, using the Mediterranean mussel (Mytilus galloprovincialis), was employed to evaluate anthropogenic chemical contamination in the North Ionian Sea, a still poorly studied area, by comparing mussel health status before (PrePT) and after (PostPT) the peak tourist season. Bioaccumulation of metal(loid)s was quantified in whole organisms. Oxidative stress was assessed in the gills and digestive gland through catalase (CAT), superoxide dismutase (SOD), lipid peroxidation (LPO), and oxidized carbonyl proteins (OMP). Neurotoxicity was evaluated via acetylcholinesterase (AChE) activity, while gene expression of stress-related biomarkers was analysed for metallothioneins (mt10, mt20), sod, cat, Glutathione S-transferase (gst), and Heat Shock Protein 70 (hsp70). Results suggest a progressive contaminant accumulation likely associated with intensified summer anthropogenic activity. Biomarker responses revealed clear activation of oxidative stress, with tissue-specific patterns. The findings confirm the effectiveness of active biomonitoring and multibiomarker approach in assessing coastal water quality and provide valuable baseline data for the management of marine ecosystems.<\/jats:p>","DOI":"10.3390\/jox16030104","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:52:30Z","timestamp":1780573950000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tissue-Specific Biomarkers and Bioaccumulation in Mytilus galloprovincialis: Seasonal Anthropogenic Stress in the North Ionian Sea (Calabria, Italy)"],"prefix":"10.3390","volume":"16","author":[{"given":"Maria Assunta","family":"Iovine","sequence":"first","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1218-0358","authenticated-orcid":false,"given":"Mariacristina","family":"Filice","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6932-5119","authenticated-orcid":false,"given":"Luisa","family":"Albarano","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, Complesso Universitario di Monte Sant\u2019Angelo, Via Cinthia n.21, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Caferro","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3389-4085","authenticated-orcid":false,"given":"Sandra","family":"Imbrogno","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Mazza","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, Complesso Universitario di Monte Sant\u2019Angelo, Via Cinthia n.21, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3978-6170","authenticated-orcid":false,"given":"Maria","family":"Costantini","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Villa Comunale, 80112 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9766-8784","authenticated-orcid":false,"given":"Valerio","family":"Zupo","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology, Ischia Marine Centre, Stazione Zoologica Anton Dohrn, 80077 Ischia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4908-0633","authenticated-orcid":false,"given":"Alfonsina","family":"Gattuso","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8507-900X","authenticated-orcid":false,"given":"Giovanni","family":"Libralato","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, Complesso Universitario di Monte Sant\u2019Angelo, Via Cinthia n.21, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carmela","family":"Cerra","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119440","DOI":"10.1016\/j.envres.2024.119440","article-title":"Comprehensive Review on Toxic Heavy Metals in the Aquatic System: Sources, Identification, Treatment Strategies, and Health Risk Assessment","volume":"258","author":"Saravanan","year":"2024","journal-title":"Environ. 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