{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T12:40:31Z","timestamp":1786452031495,"version":"build-2736575974"},"reference-count":71,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Xenobiot.","JoX"],"abstract":"<jats:p>Strobilurin fungicides are among the most widely used agrochemicals worldwide. Dimoxystrobin has been shown to induce hepatic injury in adult zebrafish; however, whether this damage also involves early extracellular matrix remodelling and hepatobiliary remodelling remains unknown. Here, we investigated early remodelling of the extracellular matrix, focusing on initial collagen deposition and glycosaminoglycan redistribution after a short-term exposure (96 h) to two environmentally relevant concentrations of dimoxystrobin (6.56 and 13.13 \u00b5g\/L) on the liver of Danio rerio using an integrated histological, histochemical, and semi-quantitative approach. The results showed a progressive dose-dependent alteration of hepatic architecture, accompanied by a significant increase in bile pigment-like deposits and collagen deposition. Exposure also induced a significant increase in the deposition of acidic glycosaminoglycans and a severe depletion of glycogen-dependent Periodic Acid\u2013Schiff positivity components, accompanied by an increase in amylase-resistant Periodic Acid\u2013Schiff reactive components. Overall, these findings indicate that dimoxystrobin-induced hepatotoxicity extends beyond parenchymal degeneration to encompass coordinated stromal and hepatobiliary remodelling. The present study also indicates that the periductal microenvironment may represent a sensitive target of dimoxystrobin toxicity and supports the use of histochemical and semi-quantitative analyses as sensitive tools for detecting sublethal pesticide-induced early extracellular matrix remodelling in fish liver.<\/jats:p>","DOI":"10.3390\/jox16040148","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:51:36Z","timestamp":1786449096000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Histochemical and Semi-Quantitative Evidence of Dimoxystrobin-Induced Liver Extracellular Matrix Remodelling in Adult Zebrafish"],"prefix":"10.3390","volume":"16","author":[{"given":"Ilaria","family":"Olivito","sequence":"first","affiliation":[{"name":"Department of Biology, Ecology and Earth Science (DiBEST), University of Calabria, Via P. Bucci 4\/B, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0719-0292","authenticated-orcid":false,"given":"Valentina","family":"Basile","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Science (DiBEST), University of Calabria, Via P. Bucci 4\/B, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Paolo Maria","family":"Graziani","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Science (DiBEST), University of Calabria, Via P. Bucci 4\/B, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naouel","family":"Gharbi","sequence":"additional","affiliation":[{"name":"Fish Biology and Aquaculture Group, Ocean and Environment Department, NORCE Norwegian Research Center, 5006 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4121-9837","authenticated-orcid":false,"given":"Rachele","family":"Macirella","sequence":"additional","affiliation":[{"name":"Department of Biology, Ecology and Earth Science (DiBEST), University of Calabria, Via P. Bucci 4\/B, 87036 Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1021\/acs.est.8b04392","article-title":"Fungicides: An overlooked pesticide class?","volume":"53","author":"Zubrod","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"123678","DOI":"10.1016\/j.envpol.2024.123678","article-title":"Current trends and mismatches on fungicide use and assessment of the ecological effects in freshwater ecosystems","volume":"347","author":"Cuco","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_3","first-page":"33","article-title":"Fungicides in agriculture and their side effects on soil enzyme activities: A review","volume":"29","author":"Kenarova","year":"2023","journal-title":"Bulg. J. Agric. Sci."},{"key":"ref_4","unstructured":"Sch\u00e4fer, R.B., Van den Brink, P.J., and Liess, M. (2012). Impacts of pesticides on freshwater ecosystems. Ecological Impacts of Toxic Chemicals, Bentham Science Publishers."},{"key":"ref_5","first-page":"e67749","article-title":"A scientific review of pesticides: Classification, toxicity, health effects, sustainability, and environmental impact","volume":"16","author":"Garud","year":"2024","journal-title":"Cureus"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"180385","DOI":"10.1016\/j.scitotenv.2025.180385","article-title":"Agrochemical fungicides as environmental drivers of antifungal resistance","volume":"1000","author":"Parra","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zhang, B., Feng, S., Gao, Y., Xie, W., Chen, Y., and Song, S. (2025). First Human Biomonitoring Evidence of Strobilurin Fungicide Exposure in South China: Impact on Oxidative Stress and Liver Damage. Toxics, 13.","DOI":"10.2139\/ssrn.5212784"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Musso, L., Fabbrini, A., and Dallavalle, S. (2020). Natural compound-derived cytochrome bc1 complex inhibitors as antifungal agents. Molecules, 25.","DOI":"10.3390\/molecules25194582"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e10001","DOI":"10.48130\/newcontam-0025-0002","article-title":"A brief review of strobilurin fungicides: Environmental exposure, transformation, and toxicity","volume":"1","author":"Liu","year":"2025","journal-title":"New Contam."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"152706","DOI":"10.1016\/j.tox.2021.152706","article-title":"Relative comparison of strobilurin fungicides at environmental levels: Focus on mitochondrial function and larval activity in early staged zebrafish (Danio rerio)","volume":"452","author":"Li","year":"2021","journal-title":"Toxicology"},{"key":"ref_11","first-page":"063","article-title":"Strobilurins: New group of fungicides","volume":"5","author":"Selim","year":"2021","journal-title":"Int. J. Plant Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"111848","DOI":"10.1016\/j.ecoenv.2020.111848","article-title":"Chronic effects of the strobilurin fungicide azoxystrobin in the leaf shredder Gammarus fossarum (Crustacea; Amphipoda) via two effect pathways","volume":"209","author":"Konschak","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2023). Peer review of the pesticide risk assessment of the active substance dimoxystrobin. EFSA J., 21, e08329.","DOI":"10.2903\/j.efsa.2023.8329"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"14387","DOI":"10.1021\/acs.est.2c02261","article-title":"National assessment of long-term groundwater response to pesticide regulation","volume":"56","author":"Kim","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.watres.2015.07.021","article-title":"The legacy of pesticide pollution: An overlooked factor in current risk assessments of freshwater systems","volume":"84","author":"Rasmussen","year":"2015","journal-title":"Water Res."},{"key":"ref_16","unstructured":"(2026, February 12). Pesticide Properties DataBase (PPDB). Dimoxystrobin (Ref: BAS 505F). Available online: https:\/\/sitem.herts.ac.uk\/aeru\/ppdb\/en\/Reports\/246.htm."},{"key":"ref_17","unstructured":"CLH Report (2026, July 29). Proposal for Harmonised Classification and Labelling Based on Regulation (EC) No 1272\/2008 (CLP Regulation), Annex VI, Part 2 Substance Name: Dimoxystrobin. Available online: https:\/\/echa.europa.eu\/documents\/10162\/71711b06-bc63-d700-6553-dd633d93ea8b."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"750","DOI":"10.33218\/001c.21978","article-title":"Zebrafish (Danio rerio) as an ecotoxicological model for Nanomaterial induced toxicity profiling","volume":"4","author":"Verma","year":"2021","journal-title":"Precis. Nanomed."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Li, H., Liu, Y., Chen, Q., Jin, L., and Peng, R. (2023). Research progress of zebrafish model in aquatic ecotoxicology. Water, 15.","DOI":"10.3390\/w15091735"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/bs.ctdb.2016.10.007","article-title":"Zebrafish in toxicology and environmental health","volume":"124","author":"Bambino","year":"2017","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"16243","DOI":"10.1007\/s11356-015-5362-1","article-title":"Danio rerio as a model in aquatic toxicology and sediment research","volume":"22","author":"Hollert","year":"2015","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The zebrafish reference genome sequence and its relationship to the human genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e14285","DOI":"10.1016\/j.heliyon.2023.e14557","article-title":"Effectiveness of zebrafish models in understanding human diseases-A review of models","volume":"9","author":"Adhish","year":"2023","journal-title":"Heliyon"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1111\/bph.15473","article-title":"Zebrafish as a tractable model of human cardiovascular disease","volume":"179","author":"Bowley","year":"2022","journal-title":"Br. J. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"116493","DOI":"10.1016\/j.ecoenv.2024.116493","article-title":"Effect of short-term exposure to the strobilurin fungicide dimoxystrobin: Morphofunctional, behavioural and mitochondrial alterations in Danio rerio embryos and larvae","volume":"279","author":"Ahmed","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"138914","DOI":"10.1016\/j.chemosphere.2023.138914","article-title":"Short-term effects of the strobilurin fungicide dimoxystrobin on zebrafish gills: A morpho-functional study","volume":"333","author":"Ahmed","year":"2023","journal-title":"Chemosphere"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Macirella, R., Ahmed, A.I.M., Talarico, F., Gharbi, N., Mezzasalma, M., and Brunelli, E. (2024). Morphological alterations and oxidative stress induction in Danio rerio liver after short-term exposure to the strobilurin fungicide dimoxystrobin. Environments, 11.","DOI":"10.3390\/environments11120282"},{"key":"ref_28","unstructured":"McCarthy, J.F., and Shugart, L.R. (1990). Liver structural alterations for pathology in fishes. Biomarkers of Environmental Contamination, CRC Press\/Lewis Publishers."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Genten, F., Terwinghe, E., and Danguy, A. (2009). Digestive system. Atlas of Fish Histology, Science Publishers. Chapter 7.","DOI":"10.1201\/9780367803599"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110336","DOI":"10.1016\/j.cbpc.2025.110336","article-title":"Transcriptomic profiling reveals tetrabromobisphenol A-induced dysregulation of hepatic lipid homeostasis in common carp (Cyprinus carpio)","volume":"299","author":"Li","year":"2025","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ma, H., Wu, W., Zhang, W., Li, H., Pan, Z., Wang, C., Gao, R., Ji, Q., Chen, Z., and You, H. (2026). The extracellular matrix in liver regeneration: Biological and therapeutic insights. Bioengineering, 13.","DOI":"10.20944\/preprints202602.0248.v1"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1089\/ars.2013.5697","article-title":"Extracellular matrix and liver disease","volume":"21","author":"Arriazu","year":"2014","journal-title":"Antioxid. Redox Signal"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1172\/JCI24282","article-title":"Liver fibrosis","volume":"115","author":"Bataller","year":"2005","journal-title":"J. Clin. Investig."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3892\/ijmm.2026.5733","article-title":"Beyond hepatic stellate cell heterogeneity: Resolving fibrosis, restoring regeneration","volume":"57","author":"Gao","year":"2026","journal-title":"Int. J. Mol. Med."},{"key":"ref_35","unstructured":"Watson, R.R., Preedy, V.R., and Zibadi, S. (2014). Chapter 51-Protection and Reversal of Hepatic Fibrosis by Polyphenols. Polyphenols in Human Health and Disease, Academic Press."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s43152-021-00030-3","article-title":"Extracellular matrix remodeling in chronic liver disease","volume":"2","author":"Ortiz","year":"2021","journal-title":"Curr. Tissue Microenviron. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1172\/JCI93557","article-title":"Extracellular matrix as a driver of progressive fibrosis","volume":"128","author":"Herrera","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e70087","DOI":"10.1111\/cpr.70087","article-title":"Deciphering Age-Dependent ECM Remodelling in Liver: Proteomic Profiling and Its Implications for Aging and Therapeutic Targets","volume":"58","author":"Liu","year":"2025","journal-title":"Cell Prolif."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"728","DOI":"10.21037\/atm-20-2948","article-title":"It\u2019s all about the spaces between cells: Role of extracellular matrix in liver fibrosis","volume":"9","author":"Khurana","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"108885","DOI":"10.1016\/j.fsi.2023.108885","article-title":"Liver fibrosis in fish research: From an immunological perspective","volume":"139","author":"Cao","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"ref_41","unstructured":"Gomez Cortes, L., Marinov, D., Sanseverino, I., Navarro Cuenca, A., Niegowska, M., Porcel Rodriguez, E., and Lettieri, T. (2020). Selection of Substances for the 3rd Watch List under the Water Framework Directive, Publications Office of the European Union. Available online: https:\/\/data.europa.eu\/doi\/10.2760\/194067."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"168427","DOI":"10.1016\/j.scitotenv.2023.168427","article-title":"Evidence of strobilurin fungicides and their metabolites in Dongjiang River ecosystem, southern China: Bioaccumulation and ecological risks","volume":"908","author":"Zhao","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_43","unstructured":"Organisation for Economic Co-Operation and Development (OECD) (2025). Test No. 203: Fish, Acute Toxicity Test. OECD Guidelines for the Testing of Chemicals, Section 2, OECD Publishing."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1369\/0022155415592180","article-title":"Image analysis or stereology: Which to choose for quantifying fibrosis?","volume":"63","author":"Marcos","year":"2015","journal-title":"J. Histochem. Cytochem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.neuro.2022.06.002","article-title":"Neurotoxicity assessment of QoI strobilurin fungicides azoxystrobin and trifloxystrobin in human SH-SY5Y neuroblastoma cells: Insights from lipidomics and mitochondrial bioenergetics","volume":"91","author":"Nguyen","year":"2022","journal-title":"Neurotoxicology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"116671","DOI":"10.1016\/j.envpol.2021.116671","article-title":"A comprehensive review of strobilurin fungicide toxicity in aquatic species: Emphasis on mode of action from the zebrafish model","volume":"275","author":"Wang","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"100282","DOI":"10.1016\/j.fhfh.2026.100282","article-title":"From waste streams to bioactive treasures: The sustainable journey of glycosaminoglycans","volume":"9","author":"Naghdi","year":"2026","journal-title":"Food Hydrocoll. Health"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1080\/01926230590890187","article-title":"A brief overview of nonneoplastic hepatic toxicity in fish","volume":"33","author":"Wolf","year":"2005","journal-title":"Toxicol. Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1053\/j.gastro.2014.07.018","article-title":"Cell death and cell death responses in liver disease: Mechanisms and clinical relevance","volume":"147","author":"Luedde","year":"2014","journal-title":"Gastroenterology"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Whalen, C., Verma, A., Kurashima, K., Carter, J., Nazzal, H., and Jain, A. (2024). Novel models for assessing and pathophysiology of hepatic ischemia\u2013reperfusion injury mechanisms. Medicina, 60.","DOI":"10.3390\/medicina60091507"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"417590","DOI":"10.1155\/2015\/417590","article-title":"Autophagy and liver ischemia\u2013reperfusion injury","volume":"2015","author":"Cursio","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Melrose, J. (2025). Glycosaminoglycans, instructive biomolecules that regulate cellular activity and synaptic neuronal control of specific tissue functional properties. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062554"},{"key":"ref_53","first-page":"320","article-title":"Glycosaminoglycans in tissue regeneration: Insights into glycobiology and their biomedical application","volume":"60","author":"Kasarinaite","year":"2026","journal-title":"Bioact. Mater."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wang, Q., and Chi, L. (2022). The alterations and roles of glycosaminoglycans in human diseases. Polymers, 14.","DOI":"10.3390\/polym14225014"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Proudfoot, A.E.I., Johnson, Z., Bonvin, P., and Handel, T.M. (2017). Glycosaminoglycan interactions with chemokines add complexity to a complex system. Pharmaceuticals, 10.","DOI":"10.3390\/ph10030070"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1016\/j.intimp.2005.04.010","article-title":"Modulation of acute inflammation by targeting glycosaminoglycan\u2013cytokine interactions","volume":"5","author":"Cripps","year":"2005","journal-title":"Int. Immunopharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Akk\u0131z, H., Gieseler, R.K., and Canbay, A. (2024). Liver fibrosis: From basic science towards clinical progress, focusing on the central role of hepatic stellate cells. Int. J. Mol. Sci., 25.","DOI":"10.20944\/preprints202405.1666.v1"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Wang, X., Copmans, D., and de Witte, P.A.M. (2021). Using zebrafish as a disease model to study fibrotic disease. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22126404"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"102741","DOI":"10.1016\/j.aqrep.2025.102741","article-title":"Dietary kelp meal alleviates feed starch intolerance of hybrid snakehead (Channa maculata \u00d7 Channa argus) by improving liver glycolipid metabolism and metabolite profile","volume":"42","author":"Wang","year":"2025","journal-title":"Aquac. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zheng, L., Wang, Z., Zhang, B., Yan, L., Wang, P., Zhao, C., Lin, H., Qiu, L., and Zhou, C. (2023). Effects of high dietary carbohydrate levels on growth performance, enzyme activities, expression of genes related to liver glucose metabolism, and the intestinal microbiota of Lateolabrax maculatus juveniles. Fishes, 8.","DOI":"10.3390\/fishes8090431"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"7589827","DOI":"10.1155\/2023\/7589827","article-title":"Data mining evidences variabilities in glucose and lipid metabolism among fish strains: A case study on three genotypes of gibel carp fed by different carbohydrate sources","volume":"2023","author":"Song","year":"2023","journal-title":"Aquac. Nutr."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Li, X., Han, T., Zheng, S., and Wu, G. (2021). Hepatic glucose metabolism and its disorders in fish. Recent Advances in Animal Nutrition and Metabolism, Springer International Publishing.","DOI":"10.1007\/978-3-030-85686-1_11"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.cld.2020.04.001","article-title":"Pathology in patients with chronic liver disease: A practical approach to liver biopsy interpretation in patients with acute and chronic liver diseases","volume":"24","author":"Rao","year":"2020","journal-title":"Clin. Liver Dis."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Kipp, Z.A., Pauss, S.N., Martinez, G.J., Hinds, T.D., and Lee, W.H. (2025). Bilirubin hepatic and intestinal transport and catabolism: Physiology, pathophysiology, and benefits. Antioxidants, 14.","DOI":"10.3390\/antiox14111326"},{"key":"ref_65","first-page":"480","article-title":"Blood performance of jaundice catfish Clarias gariepinus","volume":"12","author":"Hastuti","year":"2019","journal-title":"AACL Bioflux"},{"key":"ref_66","unstructured":"Kalakonda, A., Jenkins, B.A., and John, S. (2026, March 02). Physiology, bilirubin, StatPearls [Internet], Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK470290\/."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00011-025-02049-w","article-title":"Bilirubin metabolism and its application in disease prevention: Mechanisms and research advances","volume":"74","author":"Zhang","year":"2025","journal-title":"Inflamm. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"250","DOI":"10.19027\/jai.23.2.250-261","article-title":"Histopathology of liver, kidney, intestine, spleen, and bile of catfish with jaundice","volume":"23","author":"Asrido","year":"2024","journal-title":"J. Akuakultur Indones."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1007\/s10646-026-03085-w","article-title":"From cells to tadpoles: Mechanistic insights into ZnO-nanoparticle-induced oxidative stress and developmental toxicity in Bufo bufo","volume":"35","author":"Vava","year":"2026","journal-title":"Ecotoxicology"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S1089-3261(05)70114-8","article-title":"Mechanisms of cholestasis","volume":"4","author":"Meier","year":"2000","journal-title":"Clin. Liver Dis."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.mayocp.2015.03.017","article-title":"The cholangiopathies","volume":"90","author":"Lazaridis","year":"2015","journal-title":"Mayo Clin. Proc."}],"container-title":["Journal of Xenobiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2039-4713\/16\/4\/148\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T12:17:23Z","timestamp":1786450643000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2039-4713\/16\/4\/148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,11]]},"references-count":71,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["jox16040148"],"URL":"https:\/\/doi.org\/10.3390\/jox16040148","relation":{},"ISSN":["2039-4713"],"issn-type":[{"value":"2039-4713","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,11]]}}}