{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T22:43:54Z","timestamp":1769553834545,"version":"3.49.0"},"reference-count":61,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Polycystic ovary syndrome (PCOS) is a disorder with a symptomatic manifestation of an array of metabolic and endocrine impairments. PCOS has a relatively high prevalence rate among young women of reproductive age and is a risk factor for some severe metabolic diseases such as T2DM, insulin insensitivity, and obesity, while the most dominant endocrine malfunction is an excess of testosterone showing hyperandrogenism and hirsutism. MicroRNAs have been implicated as mediators of metabolic diseases including obesity and insulin resistance, as these can regulate multiple cellular pathways such as insulin signaling and adipogenesis. Genome-wide association studies during the last few years have also linked the Kr\u00fcpple-like family of transcription factors such as KLF14, which contribute in mechanisms of mammalian gene regulation, with certain altered metabolic traits and risk of atherosclerosis and type-2 DM. This study has characterized the biochemical and endocrine parameters in PCOS patients with a comprehensive serum profiling in comparison to healthy controls and further examined the influence of allelic variations for miRNAs 27a (rs895819 A &gt; G), 196a2 (rs11614913 C &gt; T), 423 (rs6505162C &gt; A), and transcription factor KLF14 (rs972283 A &gt; G) gene polymorphism on the risk and susceptibility to PCOS. The experimental protocol included amplification refractory mutation-specific (ARMS)-PCR to detect and determine the presence of these polymorphic variants in the study subjects. The results in this case\u2013control study showed that most of the serum biomarkers, both biochemical and endocrine, that were analyzed in the study demonstrated statistically significant alterations in PCOS patients, including lipids (LDL, HDL, cholesterol), T2DM markers (fasting glucose, free insulin, HOMA-IR), and hormones (FSH, LH, testosterone, and progesterone). The distribution of Kr\u00fcppel-like factor 14 rs972283 G &gt; A, miR-27a rs895819 A &gt; G, and miR-196a-2 rs11614913 C &gt; T genotypes analyzed within PCOS patients and healthy controls in the considered population was significant (p &lt; 0.05), except for miR-423 rs6505162 C &gt; A genotypes (p &gt; 0.05). The study found that in the codominant model, KLF14-AA was strongly associated with greater PCOS susceptibility (OR 2.35, 95% CI = 1.128 to 4.893, p &lt; 0.022), miR-27a-GA was linked to an enhanced PCOS susceptibility (OR 2.06, 95% CI = 1.165 to 3.650, p &lt; 0.012), and miR-196a-CT was associated with higher PCOS susceptibility (OR 2.06, 95% CI = 1.191 to 3.58, p &lt; 0.009). Moreover, allele A of KLF-14 and allele T of miR-196a2 were strongly associated with PCOS susceptibility in the considered population.<\/jats:p>","DOI":"10.3390\/jpm12040586","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T05:11:34Z","timestamp":1649221894000},"page":"586","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Clinical Implications of Kr\u00fcpple-like Transcription Factor KLF-14 and Certain Micro-RNA (miR-27a, miR-196a2, miR-423) Gene Variations as a Risk Factor in the Genetic Predisposition to PCOS"],"prefix":"10.3390","volume":"12","author":[{"given":"Rashid","family":"Mir","sequence":"first","affiliation":[{"name":"Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"},{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9281-1550","authenticated-orcid":false,"given":"Nizar H.","family":"Saeedi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed M.","family":"Jalal","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3394-0686","authenticated-orcid":false,"given":"Malik A.","family":"Altayar","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jameel","family":"Barnawi","sequence":"additional","affiliation":[{"name":"Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"},{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1095-5455","authenticated-orcid":false,"given":"Abdullah","family":"Hamadi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8906-198X","authenticated-orcid":false,"given":"Faris J.","family":"Tayeb","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanad E.","family":"Alshammari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology & Toxicology, Faculty of Pharmacy, University of Hail, Hail 55476, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabil","family":"Mtiraoui","sequence":"additional","affiliation":[{"name":"Laboratory of Human Genome and Multifactorial Diseases, Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed Eltigani","family":"M. Ali","sequence":"additional","affiliation":[{"name":"King Salman Military Hospital, Tabuk 47512, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8073-6461","authenticated-orcid":false,"given":"Faisel M.","family":"Abuduhier","sequence":"additional","affiliation":[{"name":"Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"},{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8059-5571","authenticated-orcid":false,"given":"Mohammad Fahad","family":"Ullah","sequence":"additional","affiliation":[{"name":"Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"},{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Tabuk 71491, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1089\/jwh.2014.5000","article-title":"Prevalence of infertility and use of fertility treatment in women with polycystic ovary syndrome: Data from a large community-based cohort study","volume":"24","author":"Joham","year":"2015","journal-title":"J. Women\u2019s Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/nrendo.2018.24","article-title":"Polycystic ovary syndrome: Definition, aetiology, diagnosis and treatment","volume":"14","year":"2018","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"765","DOI":"10.6061\/clinics\/2015(11)09","article-title":"Treatment of infertility in women with polycystic ovary syndrome: Approach to clinical practice","volume":"70","author":"Melo","year":"2015","journal-title":"Clinics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/humrep\/des463","article-title":"Women with polycystic ovary syndrome have intrinsic insulin resistance on euglycaemic-hyperinsulaemic clamp","volume":"28","author":"Stepto","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1477-7827-11-56","article-title":"Obstetric complications in women with polycystic ovary syndrome: A systematic review and meta-analysis","volume":"11","author":"Qin","year":"2013","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/1471-0528.12558","article-title":"Increased risk of preterm delivery and pre-eclampsia in women with polycystic ovary syndrome and hyperandrogenaemia","volume":"121","author":"Naver","year":"2014","journal-title":"BJOG"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Liu, Y. (2018). Polymorphisms, Genetic Diversity and Disease Susceptibility, IntechOpen.","DOI":"10.5772\/intechopen.72269"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1136\/jmg.2005.032557","article-title":"A combination of genetic polymorphisms increases the risk of progressive disease in chronic hepatitis C","volume":"42","author":"Richardson","year":"2005","journal-title":"J. Med. Genet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/ijo.2015.170","article-title":"MicroRNAs as regulators of metabolic disease: Pathophysiologic significance and emerging role as biomarkers and therapeutics","volume":"40","author":"Deiuliis","year":"2016","journal-title":"Int. J. Obes."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most mammalian mRNAs are conserved targets of microRNAs","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1111\/j.1349-7006.2010.01683.x","article-title":"The microRNA pathway and cancer","volume":"101","author":"Kwak","year":"2010","journal-title":"Cancer Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/1753-0407.12143","article-title":"Type 2 diabetes mellitus-related genetic polymorphisms in microRNAs and microRNA target sites","volume":"6","author":"Gong","year":"2014","journal-title":"J. Diabetes"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Elfaki, I., Mir, R., Mir, M.M., AbuDuhier, F.M., Babakr, A.T., and Barnawi, J. (2019). Potential Impact of MicroRNA Gene Polymorphisms in the Pathogenesis of Diabetes and Atherosclerotic Cardiovascular Disease. J. Pers. Med., 9.","DOI":"10.3390\/jpm9040051"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12958-020-00687-9","article-title":"Non-coding RNAs in polycystic ovary syndrome: A systematic review and meta-analysis","volume":"19","author":"Mu","year":"2021","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.3389\/fimmu.2018.01316","article-title":"MicroRNA-Related Polymorphisms in Infectious Diseases-Tiny Changes with a Huge Impact on Viral Infections and Potential Clinical Applications","volume":"9","author":"Ellwanger","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e9846","DOI":"10.1097\/MD.0000000000009846","article-title":"Association of microRNA-423 rs6505162 C>A polymorphism with susceptibility and metastasis of colorectal carcinoma","volume":"97","author":"Jia","year":"2018","journal-title":"Medicine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"186","DOI":"10.3389\/fgene.2019.00186","article-title":"Association of mir-196a-2 rs11614913 and mir-149 rs2292832 Polymorphisms with Risk of Cancer: An Updated Meta-Analysis","volume":"10","author":"Choupani","year":"2019","journal-title":"Front. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s40659-021-00349-z","article-title":"Association between single-nucleotide polymorphisms in miRNA and breast cancer risk: An updated review","volume":"54","author":"Arancibia","year":"2021","journal-title":"Biol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1210\/en.2017-00219","article-title":"Functional Characterization of MicroRNA-27a-3p Expression in Human Polycystic Ovary Syndrome","volume":"159","author":"Wang","year":"2018","journal-title":"Endocrinology."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1016\/j.cellsig.2015.07.019","article-title":"Kr\u00fcppel-like factor 14 increases insulin sensitivity through activation of PI3K\/Akt signal pathway","volume":"27","author":"Yang","year":"2015","journal-title":"Cell. Signal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/s41588-018-0088-x","article-title":"Regulatory variants at KLF14 influence type 2 diabetes risk via a female-specific effect on adipocyte size and body composition","volume":"50","author":"Small","year":"2018","journal-title":"Nat. Genet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/nature08625","article-title":"Parental origin of sequence variants associated with complex diseases","volume":"462","author":"Kong","year":"2009","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.placenta.2019.09.013","article-title":"Klf14 is an imprinted transcription factor that regulates placental growth","volume":"88","author":"Koppes","year":"2019","journal-title":"Placenta"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"The Rotterdam ESHRE\/ASRM-Sponsored PCOS Consensus Workshop Group (2004). Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil. Steril., 81, 19\u201325.","DOI":"10.1016\/j.fertnstert.2003.10.004"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1080\/09513590.2019.1632830","article-title":"Adiponectin (ADIPOQ) gene variants and haplotypes in Saudi Arabian women with polycystic ovary syndrome (PCOS): A case\u2013control study","volume":"36","author":"Ezzidi","year":"2020","journal-title":"Gynecol. Endocrinol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.yexmp.2015.06.009","article-title":"Assessment of association between genetic variants in microRNA genes hsa-miR-499, hsa-miR-196a2 and hsa-miR-27a and prostate cancer risk in Serbian population","volume":"99","author":"Cidilko","year":"2015","journal-title":"Exp. Mol. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.2174\/1871530318666181005095724","article-title":"Chahal SMS Potential Impact of MicroRNA-423 Gene Variability in Coronary Artery Disease","volume":"19","author":"Jha","year":"2019","journal-title":"Endocr. Metab. Immune Disord. Drug Targets"},{"key":"ref_28","first-page":"e4145","article-title":"Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the KLF14 Gene","volume":"4","author":"Zabala","year":"2017","journal-title":"Open Access Libr. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"319","DOI":"10.4314\/tjpr.v17i2.17","article-title":"Association of single nucleotide polymorphisms in miR- 499 and miR-196a with susceptibility to breast cancer","volume":"17","author":"Doulah","year":"2018","journal-title":"Tropical. J. Pharm. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mir, R., Elfaki, I., Duhier, F.M.A., Alotaibi, M.A., AlAlawy, A.I., Barnawi, J., Babakr, A.T., Mir, M.M., Mirghani, H., and Hamadi, A. (2021). Molecular Determination of mirRNA-126 rs4636297, Phosphoinositide-3-Kinase Regulatory Subunit 1-Gene Variability rs7713645, rs706713 (Tyr73Tyr), rs3730089 (Met326Ile) and Their Association with Susceptibility to T2D. J. Pers. Med., 11.","DOI":"10.3390\/jpm11090861"},{"key":"ref_31","first-page":"755","article-title":"Polycystic Ovary Syndrome, A Complex Entity Involving Reproductive and Metabolic Impairments: A Systematic Review","volume":"120","author":"Chelaru","year":"2016","journal-title":"Rev. Med. Chir. Soc. Med. Nat. Iasi."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Teede, H., Deeks, A., and Moran, L. (2010). Polycystic ovary syndrome: A complex condition with psychological, reproductive and metabolic manifestations that impacts on health across the lifespan. BMC Med., 8.","DOI":"10.1186\/1741-7015-8-41"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.fertnstert.2018.05.004","article-title":"Recom-mendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome","volume":"110","author":"Teede","year":"2018","journal-title":"Fertil Steril."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"516","DOI":"10.3389\/fendo.2020.00516","article-title":"Fundamental Concepts and Novel Aspects of Polycystic Ovarian Syndrome: Expert Consensus Resolutions","volume":"11","author":"Aversa","year":"2020","journal-title":"Front. Endocrinol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"R71","DOI":"10.1530\/EC-18-0502","article-title":"Complex diseases and co-morbidities: Polycystic ovary syndrome and type 2 diabetes mellitus","volume":"8","author":"Rodgers","year":"2019","journal-title":"Endocr. Connect."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1038\/ijo.2017.126","article-title":"Metabolic profiling of polycystic ovary syndrome reveals interactions with abdominal obesity","volume":"41","author":"Valcarcel","year":"2017","journal-title":"Int. J. Obes."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"137","DOI":"10.5468\/ogs.2013.56.3.137","article-title":"Dyslipidemia in women with polycystic ovary syndrome","volume":"56","author":"Kim","year":"2013","journal-title":"Obstet. Gynecol. Sci."},{"key":"ref_38","first-page":"3389","article-title":"Altered composition of high density lipoproteins in women with the polycystic ovary syndrome","volume":"82","author":"Rajkhowa","year":"1997","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1365-2265.1994.tb02577.x","article-title":"Serum lipoprotein lipid profile in women with the polycystic ovary syndrome: Relation to anthropometric, endocrine and metabolic variables","volume":"41","author":"Holte","year":"1994","journal-title":"Clin. Endocrinol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1210\/en.2019-00428","article-title":"Neuroendocrine Impairments of Polycystic Ovary Syndrome","volume":"160","author":"Ruddenklau","year":"2019","journal-title":"Endocrinology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s00109-004-0603-7","article-title":"Regulatory polymorphisms underlying complex disease traits","volume":"83","author":"Knight","year":"2005","journal-title":"J. Mol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3345","DOI":"10.1038\/onc.2009.194","article-title":"Genetic predisposition to human disease: Allele-specific expression and low-penetrance regulatory loci","volume":"28","year":"2009","journal-title":"Oncogene"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1126\/science.1159397","article-title":"Germline allele-specific expression of TGFBR1 confers an increased risk of colorectal cancer","volume":"321","author":"Valle","year":"2008","journal-title":"Science"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/ng.609","article-title":"MAGIC investigators; GIANT Consortium. Twelve type 2 diabetes susceptibility loci identified through large-scale association analysis","volume":"42","author":"Voight","year":"2010","journal-title":"Nat. Genet."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1111\/j.1538-7836.2012.04815.x","article-title":"Genome-wide association study validation identifies novel loci for atherosclerotic cardiovascular disease","volume":"10","author":"Chen","year":"2012","journal-title":"J. Thromb. Haemost."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3389\/fcvm.2020.00091","article-title":"Transcription Factor KLF14 and Metabolic Syndrome","volume":"7","author":"Yang","year":"2020","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1152\/physrev.00058.2009","article-title":"Mammalian Kr\u00fcppel-like factors in health and diseases","volume":"90","author":"McConnell","year":"2010","journal-title":"Physiol. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/ng1011-1040c","article-title":"Identification of an imprinted master trans regulator at the KLF14 locus related to multiple metabolic phenotypes","volume":"43","author":"Small","year":"2011","journal-title":"Nat. Genet."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/nature24277","article-title":"Genetic effects on gene expression across human tissues","volume":"550","author":"Aguet","year":"2017","journal-title":"Nature"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.humimm.2014.01.008","article-title":"Association of KCNQ1 and KLF14 polymorphisms and risk of type 2 diabetes mellitus: A global meta-analysis","volume":"75","author":"Wang","year":"2014","journal-title":"Hum. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"231515","DOI":"10.1155\/2013\/231515","article-title":"Association of the KLF14 rs4731702 SNP and serum lipid levels in the Guangxi Mulao and Han populations","volume":"2013","author":"Huang","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1179558119874042","DOI":"10.1177\/1179558119874042","article-title":"Obesity and Polycystic Ovary Syndrome: Implications for Pathogenesis and Novel Management Strategies","volume":"13","author":"Barber","year":"2019","journal-title":"Clin. Med. Insights Reprod. Health"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3389\/fendo.2018.00402","article-title":"Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation","volume":"9","author":"Hayder","year":"2018","journal-title":"Front. Endocrinol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/nrm3313","article-title":"MicroRNAs in metabolism and metabolic disorders","volume":"13","author":"Rottiers","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s00439-017-1858-8","article-title":"Genetic variants in microRNA genes and targets associated with cardiovascular disease risk factors in the African-American population","volume":"137","author":"Li","year":"2018","journal-title":"Hum. Genet."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"587561","DOI":"10.3389\/fendo.2020.587561","article-title":"Association of Genetic Polymorphisms in MicroRNAs With Type 2 Diabetes Mellitus in a Chinese Population","volume":"11","author":"Zhu","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3514","DOI":"10.12659\/MSM.897147","article-title":"Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion","volume":"22","author":"Wang","year":"2016","journal-title":"Med. Sci. Monit."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.3892\/mmr.2016.5495","article-title":"MiRNA polymorphisms (miR-146a, miR-149, miR-196a2 and miR-499) are associated with the risk of coronary artery disease","volume":"14","author":"Sung","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"552421","DOI":"10.3389\/fcvm.2020.552421","article-title":"Risk of Cardiovascular and Cerebrovascular Events in Polycystic Ovarian Syndrome Women: A Meta-Analysis of Cohort Studies","volume":"7","author":"Zhang","year":"2020","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"10207","DOI":"10.3389\/bjbs.2021.10207","article-title":"MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity","volume":"79","author":"Jaafar","year":"2022","journal-title":"Br. J. Biomed. Sci."},{"key":"ref_61","first-page":"516","article-title":"Towards precision medicine: Interrogating the human genome to identify drug pathways associated with potentially functional, population-differentiated polymorphisms","volume":"19","author":"Bachtiar","year":"2019","journal-title":"Pharm. J."}],"container-title":["Journal of Personalized Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4426\/12\/4\/586\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:48:50Z","timestamp":1760136530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4426\/12\/4\/586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,6]]},"references-count":61,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["jpm12040586"],"URL":"https:\/\/doi.org\/10.3390\/jpm12040586","relation":{},"ISSN":["2075-4426"],"issn-type":[{"value":"2075-4426","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,6]]}}}