{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:08:42Z","timestamp":1790266122284,"version":"4.1.0"},"reference-count":231,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Romanian Executive Agency for Higher Education, Research, Development and Innovation Funding","award":["PN-III-P4-PCE-2021-0549"],"award-info":[{"award-number":["PN-III-P4-PCE-2021-0549"]}]},{"name":"Romanian Executive Agency for Higher Education, Research, Development and Innovation Funding","award":["PN 19.29.01.01"],"award-info":[{"award-number":["PN 19.29.01.01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Skin cancer, which includes the most frequent malignant non-melanoma carcinomas (basal cell carcinoma, BCC, and squamous cell carcinoma, SCC), along with the difficult to treat cutaneous melanoma (CM), pose important worldwide issues for the health care system. Despite the improved anti-cancer armamentarium and the latest scientific achievements, many skin cancer patients fail to respond to therapies, due to the remarkable heterogeneity of cutaneous tumors, calling for even more sophisticated biomarker discovery and patient monitoring approaches. Droplet digital polymerase chain reaction (ddPCR), a robust method for detecting and quantifying low-abundance nucleic acids, has recently emerged as a powerful technology for skin cancer analysis in tissue and liquid biopsies (LBs). The ddPCR method, being capable of analyzing various biological samples, has proved to be efficient in studying variations in gene sequences, including copy number variations (CNVs) and point mutations, DNA methylation, circulatory miRNome, and transcriptome dynamics. Moreover, ddPCR can be designed as a dynamic platform for individualized cancer detection and monitoring therapy efficacy. Here, we present the latest scientific studies applying ddPCR in dermato-oncology, highlighting the potential of this technology for skin cancer biomarker discovery and validation in the context of personalized medicine. The benefits and challenges associated with ddPCR implementation in the clinical setting, mainly when analyzing LBs, are also discussed.<\/jats:p>","DOI":"10.3390\/jpm12071136","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T22:06:00Z","timestamp":1657749960000},"page":"1136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Skin Cancer Research Goes Digital: Looking for Biomarkers within the Droplets"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6464-381X","authenticated-orcid":false,"given":"Elena-Georgiana","family":"Dobre","sequence":"first","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Splaiul Independentei 91\u201395, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2870-5497","authenticated-orcid":false,"given":"Carolina","family":"Constantin","sequence":"additional","affiliation":[{"name":"Immunology Department, \u201cVictor Babes\u201d National Institute of Pathology, 050096 Bucharest, Romania"},{"name":"Pathology Department, Colentina Clinical Hospital, 020125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9339-2805","authenticated-orcid":false,"given":"Monica","family":"Neagu","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Splaiul Independentei 91\u201395, 050095 Bucharest, Romania"},{"name":"Immunology Department, \u201cVictor Babes\u201d National Institute of Pathology, 050096 Bucharest, Romania"},{"name":"Pathology Department, Colentina Clinical Hospital, 020125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/s12276-020-0412-2","article-title":"New insights into the functions of Cox-2 in skin and esophageal malignancies","volume":"52","author":"Moon","year":"2020","journal-title":"Exp. 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