{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:07:25Z","timestamp":1788343645118,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PIVET Medical Center"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Sperm DNA fragmentation (SDF) levels have been measured in the workup for in vitro fertilization (IVF) at PIVET since 2007, with the Halosperm test having replaced the previous sperm chromatin structure assay (SCSA) since 2013. Of 2624 semen samples analyzed for the Halosperm test, 57 were excluded as the sperm concentration was &lt;5 million\/mL, a level too low for accurate testing, leaving 2567 samples for assessment within this study. The SDF rates were categorized in 5 sperm DNA fragmentation indices (DFI), ranging from &lt;5% to levels &gt;30%, and these categories were correlated with the respective semen analysis profiles and two clinical parameters, namely the age of the male and the ejaculatory abstinence period prior to the sample. The results showed a significant correlation with male age (r = 0.088; p &lt; 0.0001), the abstinence period (r = 0.076; p = 0.0001), and the semen volume (r 0.063; p = 0.001), meaning an adversely high SDF was associated with advanced age, prolonged abstinence, and raised semen volume parameters. There was a significant negative correlation with sperm morphology (r = \u22120.074; p = 0.0001), progressive motility (r = \u22120.257; p &lt; 0.0001), and semen pH (r = \u22120.066; p &lt; 0.001), meaning these semen anomalies were associated with high SDF values. With respect to abnormal morphology, sperm tail defects had a positive correlation (r = 0.096; p &lt; 0.0001) while midpiece defects showed a negative correlation (r = \u22120.057; p = 0.004), meaning that tail defects are most likely to associate with adverse DFI values. With respect to motility patterns, the poorer patterns showed a positive correlation with increased DFI, namely C pattern (r = 0.055; p = 0.005) and D pattern (r = 0.253; p &lt; 0.0001). These results imply that raised DFI reflects poor sperm quality and should be investigated in clinical trials involving IVF and the consideration of intracytoplasmic sperm injection (ICSI).<\/jats:p>","DOI":"10.3390\/jpm13030518","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T03:04:46Z","timestamp":1678763086000},"page":"518","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["How Well Do Semen Analysis Parameters Correlate with Sperm DNA Fragmentation? A Retrospective Study from 2567 Semen Samples Analyzed by the Halosperm Test"],"prefix":"10.3390","volume":"13","author":[{"given":"Shiao Chuan","family":"Chua","sequence":"first","affiliation":[{"name":"PIVET Medical Centre, Perth, WA 6007, Australia"},{"name":"Hospital Shah Alam, Shah Alam 40000, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven John","family":"Yovich","sequence":"additional","affiliation":[{"name":"PIVET Medical Centre, Perth, WA 6007, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Michael","family":"Hinchliffe","sequence":"additional","affiliation":[{"name":"PIVET Medical Centre, Perth, WA 6007, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9583-3683","authenticated-orcid":false,"given":"John Lui","family":"Yovich","sequence":"additional","affiliation":[{"name":"PIVET Medical Centre, Perth, WA 6007, Australia"},{"name":"Curtin Medical School, Curtin University, Perth, WA 6102, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"ref_1","first-page":"1786","article-title":"The International Glossary on Infertility and Fertility Care, 2017","volume":"32","author":"Adamson","year":"2017","journal-title":"Hum. Reprod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.clinbiochem.2018.03.012","article-title":"Fertility and infertility: Definition and epidemiology","volume":"62","author":"Wyns","year":"2018","journal-title":"Clin. Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12610-016-0046-3","article-title":"Predictive value of sperm deoxyribonucleic acid (DNA) fragmentation index in male infertility","volume":"27","author":"Wiweko","year":"2017","journal-title":"Basic Clin. Androl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12958-015-0032-1","article-title":"A unique view on male infertility around the globe","volume":"13","author":"Agarwal","year":"2015","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/s41585-018-0003-3","article-title":"Genetics of male infertility","volume":"15","author":"Krausz","year":"2018","journal-title":"Nat. Rev. Urol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1136\/bmj.305.6854.609","article-title":"Evidence for decreasing quality of semen during past 50 years","volume":"305","author":"Carlsen","year":"1992","journal-title":"BMJ"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/00016344409154563","article-title":"Studies on impaired fertility in man with special reference to the male","volume":"24","author":"Hammen","year":"1944","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_8","unstructured":"World Health Organization (2021). WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [6th ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"356","DOI":"10.21037\/tau.2019.06.22","article-title":"The effect of sperm DNA fragmentation index on assisted reproductive technology outcomes and its relationship with semen parameters and lifestyle","volume":"8","author":"Yang","year":"2019","journal-title":"Transl. Androl. Urol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.fertnstert.2009.10.046","article-title":"Sperm DNA fragmentation: Mechanisms of origin, impact on reproductive outcome, and analysis","volume":"93","author":"Sakkas","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/humrep\/dez308","article-title":"Female ageing affects the DNA repair capacity of oocytes in IVF using a controlled model of sperm DNA damage in mice","volume":"35","author":"Horta","year":"2020","journal-title":"Hum. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1002\/rmb2.12297","article-title":"Does sperm DNA fragmentation correlate with semen parameters?","volume":"18","author":"Le","year":"2019","journal-title":"Reprod. Med. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.rbmo.2015.05.006","article-title":"Should sperm DNA fragmentation testing be included in the male infertility work-up?","volume":"31","author":"Lewis","year":"2015","journal-title":"Reprod. Biomed. Online"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/aja.2013.81","article-title":"Diagnostic value of sperm DNA fragmentation and sperm high-magnification for predicting outcome of assisted reproduction treatment","volume":"15","author":"Lopez","year":"2013","journal-title":"Asian J. Androl."},{"key":"ref_15","unstructured":"World Health Organization (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization. [5th ed]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"78","DOI":"10.3109\/19396368.2010.515704","article-title":"Are sperm chromatin and DNA defects relevant in the clinic?","volume":"57","author":"Zini","year":"2011","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e221","DOI":"10.1111\/j.1365-2605.2009.00995.x","article-title":"Sperm chromatin structure assay as an independent predictor of fertility in vivo: A case-control study","volume":"33","author":"Giwercman","year":"2010","journal-title":"Int. J. Androl."},{"key":"ref_18","first-page":"A34","article-title":"Sperm Chromatin Structure Assay: Population profile and Correlation with Semen parameters and Sperm Function Test","volume":"47","author":"Yovich","year":"2007","journal-title":"ANZJOG"},{"key":"ref_19","first-page":"554","article-title":"Total antioxidant capacity and lipid peroxidation in semen of patient with hyperviscosity","volume":"16","author":"Layali","year":"2015","journal-title":"Cell J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.ucl.2008.01.014","article-title":"Anejaculation and retrograde ejaculation","volume":"35","author":"Ohl","year":"2008","journal-title":"Urol. Clin. N. Am."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1007\/s10815-021-02080-w","article-title":"Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients","volume":"38","author":"Ferrigno","year":"2021","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/S0015-0282(16)56704-1","article-title":"Use of the acrosome reaction to ionophore challenge test in managing patients in an assisted reproduction program: A prospective, double-blind, randomized controlled study","volume":"61","author":"Yovich","year":"1994","journal-title":"Fertil. Steril."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/j.1939-4640.1991.tb00223.x","article-title":"A test of the human sperm acrosome reaction following ionophore challenge. Relationship to fertility and other seminal parameters","volume":"12","author":"Cummins","year":"1991","journal-title":"J. Androl."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3833","DOI":"10.1038\/s41598-020-60876-9","article-title":"DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice","volume":"10","author":"Li","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.rbmo.2010.06.026","article-title":"Hypo-osmotic swelling test identifies individual spermatozoa with minimal DNA fragmentation","volume":"21","author":"Stanger","year":"2010","journal-title":"Reprod. Biomed. Online"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e979623","DOI":"10.4161\/21565562.2014.979623","article-title":"Mechanisms of spermiogenesis and spermiation and how they are disturbed","volume":"4","year":"2014","journal-title":"Spermatogenesis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"412","DOI":"10.5534\/wjmh.200128","article-title":"Sperm DNA Fragmentation: A New Guideline for Clinicians","volume":"38","author":"Agarwal","year":"2020","journal-title":"World J. Mens Health"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e13874","DOI":"10.1111\/and.13874","article-title":"Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations","volume":"53","author":"Esteves","year":"2021","journal-title":"Andrologia"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/j.2047-2927.2012.00041.x","article-title":"Prevalence of high DNA fragmentation index in male partners of unexplained infertile couples","volume":"1","author":"Oleszczuk","year":"2013","journal-title":"Andrology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.rbmo.2012.09.019","article-title":"Sperm DNA damage has a negative association with live-birth rates after IVF","volume":"26","author":"Simon","year":"2013","journal-title":"Reprod. Biomed. Online"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1016\/j.fertnstert.2008.01.086","article-title":"Correlation between DNA damage and sperm parameters: A prospective study of 1633 patients","volume":"91","author":"Belloc","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e23548","DOI":"10.1002\/jcla.23548","article-title":"Analysis of age-associated alternation of SCSA sperm DNA fragmentation index and semen characteristics of 1790 subfertile males in China","volume":"34","author":"Lu","year":"2020","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_33","first-page":"246","article-title":"Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?","volume":"5","author":"Farsi","year":"2016","journal-title":"Int. J. Mol. Cell. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/and.12003","article-title":"Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulations of sperm with highly degraded DNA","volume":"45","author":"Abad","year":"2013","journal-title":"Andrologia"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"349","DOI":"10.2164\/jandrol.04146","article-title":"Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment","volume":"26","author":"Greco","year":"2005","journal-title":"J. Androl."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"375","DOI":"10.5152\/tud.2022.22058","article-title":"Antioxidant Supplementation on Sperm DNA Fragmentation and Sperm Parameters: A Systematic Review and Meta-Analysis","volume":"48","author":"Noegroho","year":"2022","journal-title":"Turk. J. Urol."}],"container-title":["Journal of Personalized Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4426\/13\/3\/518\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:53:54Z","timestamp":1760122434000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4426\/13\/3\/518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,13]]},"references-count":36,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["jpm13030518"],"URL":"https:\/\/doi.org\/10.3390\/jpm13030518","relation":{},"ISSN":["2075-4426"],"issn-type":[{"value":"2075-4426","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,13]]}}}