{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:14:25Z","timestamp":1765728865146,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T00:00:00Z","timestamp":1684627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"Sydney Children\u2019s Hospital Foundation","doi-asserted-by":"publisher","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Rebecca L. Cooper Foundation","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"Cystic Fibrosis Australia\u2014The David Millar Giles Innovation","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"Sydney Children Hospital Network Foundation","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"Luminesce Alliance Research","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"Rotary Club of Sydney Cove","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"UNSW postgraduate award scholarships","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"Australian Government Research Training Program Scholarship","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]},{"name":"UNSW Scientia program","award":["NHMRC_APP1188987"],"award-info":[{"award-number":["NHMRC_APP1188987"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Primary nasal epithelial cells and culture models are used as important diagnostic, research and drug development tools for several airway diseases. Various instruments have been used for the collection of human nasal epithelial (HNE) cells but no global consensus yet exists regarding the optimal tool. This study compares the efficiency of two cytology brushes (Olympus (2 mm diameter) and Endoscan (8 mm diameter)) in collecting HNE cells. The study involved two phases, with phase one comparing the yield, morphology and cilia beat frequency (CBF) of cells collected from paediatric participants using each of the two brushes. Phase two compared nasal brushing under general anaesthetic and in the awake state, across a wide age range, via the retrospective audit of the use of the Endoscan brush in 145 participants. Results indicated no significant difference in CBF measurements between the two brushes, suggesting that the choice of brush does not compromise diagnostic accuracy. However, the Endoscan brush collected significantly more total and live cells than the Olympus brush, making it a more efficient option. Importantly, the Endoscan brush is more cost-effective, with a notable price difference between the two brushes.<\/jats:p>","DOI":"10.3390\/jpm13050864","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T02:28:42Z","timestamp":1684722522000},"page":"864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparing Cytology Brushes for Optimal Human Nasal Epithelial Cell Collection: Implications for Airway Disease Diagnosis and Research"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7069-6179","authenticated-orcid":false,"given":"Laura K.","family":"Fawcett","sequence":"first","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"},{"name":"Department of Respiratory Medicine, Sydney Children\u2019s Hospital, Sydney, NSW 2031, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nihan","family":"Turgutoglu","sequence":"additional","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8919-5515","authenticated-orcid":false,"given":"Katelin M.","family":"Allan","sequence":"additional","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"},{"name":"School of Biomedical Sciences, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Belessis","sequence":"additional","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"},{"name":"Department of Respiratory Medicine, Sydney Children\u2019s Hospital, Sydney, NSW 2031, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Widger","sequence":"additional","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"},{"name":"Department of Respiratory Medicine, Sydney Children\u2019s Hospital, Sydney, NSW 2031, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Jaffe","sequence":"additional","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"},{"name":"Department of Respiratory Medicine, Sydney Children\u2019s Hospital, Sydney, NSW 2031, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1144-5411","authenticated-orcid":false,"given":"Shafagh A.","family":"Waters","sequence":"additional","affiliation":[{"name":"Discipline of Paediatrics and Child Health, School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"},{"name":"Department of Respiratory Medicine, Sydney Children\u2019s Hospital, Sydney, NSW 2031, Australia"},{"name":"School of Biomedical Sciences, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,21]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Miller, D., Turner, S.W., Spiteri-Cornish, D., McInnes, N., Scaife, A., Danielian, P.J., Devereux, G., and Walsh, G.M. 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