{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T19:27:07Z","timestamp":1787686027575,"version":"build-2784847793"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Background: Our aim is to describe Gallucci\u2019s (VV-G) technique for vesicourethral anastomosis and posterior musculofascial reconstruction (PMFR) during robot-assisted radical prostatectomy (RARP) and to assess early urinary continence recovery and perioperative outcomes. VV-G consists of a \u201csingle knot\u2013single running suture\u201d vesicourethral anastomosis with PMFR. Methods: Between September 2019 and October 2021, we prospectively compared VV-G vs. conventional Van Velthoven anastomosis (VV-STD) during RARP. We excluded patients with urinary incontinence, pelvic radiotherapy, and urethral and BPH surgery prior to RARP. Social continence (SC) recovery, perioperative complications, and length of hospital stay (LOS) were compared between VV-G vs. VV-STD. SC was defined as 0\u20131 pad\/die. We applied 1:1 propensity score matching (PSM) adjusting for different covariates (age, Charlson Comorbidity Index, BMI, prostate volume, nerve-sparing and lymph node dissection). Results: From 166 patients, 1:1 PSM resulted in two equally sized groups of 40 patients each with no residual differences (all p \u2265 0.2). VV-G yielded higher 3-month SC rates than VV-STD (97.5 vs. 55.0%, p &lt; 0.001). A tiny difference was still recorded at one-year follow-up (97.5 vs. 80.0%, p = 0.029, HR: 2.90, 95% CI: 1.74\u20134.85, p &lt; 0.001). Conversely, we observed no differences in any perioperative complications (15.0 vs. 22.5%, OR: 0.61, 95% CI 0.19\u20131.88, p = 0.4) and LOS (3 vs. 4 days, \u0394: \u22120.69 \u00b1 0.61, p = 0.1). Conclusions: VV-G significantly improved early SC recovery without increasing perioperative morbidity. In our opinion, VV-G represents an easy-to-learn and easy-to-teach technique due to its single-suture, single-knot, and symmetrical design.<\/jats:p>","DOI":"10.3390\/jpm13071072","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:43:30Z","timestamp":1688085810000},"page":"1072","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["\u201cSingle Knot\u2013Single Running Suture\u201d Vesicourethral Anastomosis with Posterior Musculofascial Reconstruction during Robot-Assisted Radical Prostatectomy: A Step-by-Step Guide of Surgical Technique"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3129-0544","authenticated-orcid":false,"given":"Rocco Simone","family":"Flammia","sequence":"first","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"},{"name":"Department of Urology, \u201cRegina Elena\u201d National Cancer Institute, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1140-0590","authenticated-orcid":false,"given":"Eugenio","family":"Bologna","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7586-4132","authenticated-orcid":false,"given":"Umberto","family":"Anceschi","sequence":"additional","affiliation":[{"name":"Department of Urology, \u201cRegina Elena\u201d National Cancer Institute, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Tufano","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3274-1247","authenticated-orcid":false,"given":"Leslie Claire","family":"Licari","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6067-5497","authenticated-orcid":false,"given":"Luca","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6478-8584","authenticated-orcid":false,"given":"Flavia","family":"Proietti","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"},{"name":"Department of Urology, \u201cRegina Elena\u201d National Cancer Institute, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3611-1559","authenticated-orcid":false,"given":"Federico","family":"Alviani","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Gallucci","sequence":"additional","affiliation":[{"name":"Department of Urology, \u201cRegina Elena\u201d National Cancer Institute, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4868-9025","authenticated-orcid":false,"given":"Giuseppe","family":"Simone","sequence":"additional","affiliation":[{"name":"Department of Urology, \u201cRegina Elena\u201d National Cancer Institute, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Costantino","family":"Leonardo","sequence":"additional","affiliation":[{"name":"Department of Maternal-Child and Urological Sciences, Sapienza University Rome, Policlinico Umberto I Hospital, 00161 Rome, Italy"},{"name":"Department of Urology, \u201cRegina Elena\u201d National Cancer Institute, 00128 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"a030361","DOI":"10.1101\/cshperspect.a030361","article-title":"The Epidemiology of Prostate Cancer","volume":"8","author":"Pernar","year":"2018","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int. J. Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1016\/S0022-5347(17)47661-0","article-title":"Radical prostatectomy with preservation of sexual function: Pathological findings in the first 100 cases","volume":"134","author":"Eggleston","year":"1985","journal-title":"J. Urol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1016\/j.eururo.2006.06.023","article-title":"Complications, urinary continence, and oncologic outcome of 1000 laparoscopic transperitoneal radical prostatectomies\u2014experience at the charit\u00e9 hospital berlin, campus mitte","volume":"50","author":"Lein","year":"2006","journal-title":"Eur. Urol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1056\/NEJMoa074311","article-title":"Quality of life and satisfaction with outcome among prostate-cancer survivors","volume":"358","author":"Sanda","year":"2008","journal-title":"N. Engl. J. Med."},{"key":"ref_6","first-page":"367","article-title":"Contemporary trends in the surgical management of urinary incontinence after radical prostatectomy in the United States","volume":"26","author":"Huang","year":"2022","journal-title":"Prostate Cancer Prostatic Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.eururo.2012.05.045","article-title":"Systematic review and meta-analysis of studies reporting urinary continence recovery after robot-assisted radical prostatectomy","volume":"62","author":"Ficarra","year":"2012","journal-title":"Eur. Urol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1097\/MOU.0b013e32835b0ae5","article-title":"Anatomic and technical considerations for optimizing recovery of urinary function during robotic-assisted radical prostatectomy","volume":"23","author":"Vora","year":"2013","journal-title":"Curr. Opin. Urol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.acuro.2021.06.004","article-title":"Continence definition and prognostic factors for early urinary continence recovery in posterior rhabdosphincter reconstruction after robot-assisted radical prostatectomy. Post-hoc analysis of a randomised controlled trial","volume":"46","author":"Salazar","year":"2022","journal-title":"Actas Urol. Esp."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/bju.13480","article-title":"Posterior musculofascial reconstruction after radical prostatectomy: An updated systematic review and a meta-analysis","volume":"118","author":"Grasso","year":"2016","journal-title":"BJU Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00345-012-0840-2","article-title":"Laparoscopic \u201csingle knot\u2013single running\u201d suture vesico-urethral anastomosis with posterior musculofascial reconstruction","volume":"30","author":"Simone","year":"2012","journal-title":"World J. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.eururo.2017.12.018","article-title":"Updates in the eighth edition of the tumor-node-metastasis staging classification for urologic cancers","volume":"73","author":"Paner","year":"2018","journal-title":"Eur. Urol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1097\/SLA.0b013e3181b13ca2","article-title":"The clavien-dindo classification of surgical complications: Five-year experience","volume":"250","author":"Clavien","year":"2009","journal-title":"Ann. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1016\/j.eururo.2008.12.035","article-title":"Operative details and oncological and functional outcome of robotic-assisted laparoscopic radical prostatectomy: 400 cases with a minimum of 12 months follow-up","volume":"55","author":"Murphy","year":"2009","journal-title":"Eur. Urol."},{"key":"ref_15","first-page":"99","article-title":"Evaluation of combined oncologic and functional outcomes after robotic-assisted laparoscopic extraperitoneal radical prostatectomy: Trifecta rate of achieving continence, potency and cancer control","volume":"31","author":"Xylinas","year":"2013","journal-title":"Urol. Oncol. Semin. Orig. Investig."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.eururo.2016.08.003","article-title":"EAU-ESTRO-SIOG guidelines on prostate cancer. Part 1: Screening, diagnosis, and local treatment with curative intent","volume":"71","author":"Mottet","year":"2017","journal-title":"Eur. Urol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.eururo.2011.10.044","article-title":"Updated nomogram predicting lymph node invasion in patients with prostate cancer undergoing extended pelvic lymph node dissection: The essential importance of percentage of positive cores","volume":"61","author":"Briganti","year":"2011","journal-title":"Eur. Urol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9191830","DOI":"10.1155\/2020\/9191830","article-title":"Analysis of learning curve in robot-assisted radical prostatectomy performed by a surgeon","volume":"2020","author":"Slusarenco","year":"2020","journal-title":"Adv. Urol."},{"key":"ref_19","first-page":"149.e1","article-title":"Learning curve of minimally invasive radical prostatectomy: Comprehensive evaluation and cumulative summation analysis of oncological outcomes","volume":"35","author":"Sivaraman","year":"2017","journal-title":"Urol. Oncol. Semin. Orig. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/S0090-4295(02)02543-8","article-title":"Technique for laparoscopic running urethrovesical anastomosis:the single knot method","volume":"61","author":"Ahlering","year":"2003","journal-title":"Urology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.eururo.2021.01.028","article-title":"Robot-assisted radical prostatectomy using the novel urethral fixation technique versus standard vesicourethral anastomosis","volume":"79","author":"Ficarra","year":"2021","journal-title":"Eur. Urol."},{"key":"ref_22","first-page":"127","article-title":"Personal research: Reconstruction of the urethral striated sphincter","volume":"73","author":"Rocco","year":"2001","journal-title":"Arch. Ital. Urol. Androl."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.eururo.2007.01.109","article-title":"Early continence recovery after open radical prostatectomy with restoration of the posterior aspect of the rhabdosphincter","volume":"52","author":"Rocco","year":"2007","journal-title":"Eur. Urol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1016\/S0022-5347(06)00262-X","article-title":"Restoration of posterior aspect of rhabdosphincter shortens continence time after radical retropubic prostatectomy","volume":"175","author":"Rocco","year":"2006","journal-title":"J. Urol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.eururo.2006.10.014","article-title":"Posterior reconstruction of the rhabdosphincter allows a rapid recovery of continence after transperitoneal videolaparoscopic radical prostatectomy","volume":"51","author":"Rocco","year":"2007","journal-title":"Eur. Urol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1111\/j.1464-410X.2008.08090.x","article-title":"Surgery illustrated\u2014Focus on details","volume":"102","author":"Coughlin","year":"2008","journal-title":"BJU Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.eururo.2020.09.044","article-title":"Hood technique for robotic radical prostatectomy\u2014preserving periurethral anatomical structures in the space of retzius and sparing the pouch of douglas, enabling early return of continence without compromising surgical margin rates","volume":"80","author":"Wagaskar","year":"2020","journal-title":"Eur. Urol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.eururo.2012.05.041","article-title":"Posterior musculofascial reconstruction after radical prostatectomy: A systematic review of the literature","volume":"62","author":"Rocco","year":"2012","journal-title":"Eur. Urol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.euo.2021.02.005","article-title":"A Randomised controlled trial to assess the benefit of posterior rhabdosphincter reconstruction in early urinary continence recovery after robot-assisted radical prostatectomy","volume":"5","author":"Salazar","year":"2022","journal-title":"Eur. Urol. Oncol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1111\/j.1464-410X.2008.07424.x","article-title":"Total reconstruction of the vesico-urethral junction","volume":"101","author":"Tewari","year":"2008","journal-title":"BJU Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"29","DOI":"10.4111\/kju.2012.53.1.29","article-title":"Effect of bladder neck preservation and posterior urethral reconstruction during robot-assisted laparoscopic radical prostatectomy for urinary continence","volume":"53","author":"You","year":"2012","journal-title":"Korean J. Urol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.juro.2014.10.023","article-title":"Effects of new 1-step posterior reconstruction method on recovery of continence after robot-assisted laparoscopic prostatectomy: Results of a prospective, single-blind, parallel group, randomized, controlled trial","volume":"193","author":"Jeong","year":"2015","journal-title":"J. Urol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.juro.2010.11.085","article-title":"Posterior rhabdosphincter reconstruction during robotic assisted radical prostatectomy: Results from a phase ii randomized clinical trial","volume":"185","author":"Sutherland","year":"2011","journal-title":"J. Urol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.eururo.2021.01.030","article-title":"Robotic-assisted versus laparoscopic surgery: Outcomes from the first multicentre, randomised, patient-blinded controlled trial in radical prostatectomy (Lap-01)","volume":"79","author":"Stolzenburg","year":"2021","journal-title":"Eur. Urol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1097\/PRS.0b013e318219c171","article-title":"The levels of evidence and their role in evidence-based medicine","volume":"128","author":"Burns","year":"2011","journal-title":"Plast. Reconstr. Surg."}],"container-title":["Journal of Personalized Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4426\/13\/7\/1072\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:03:29Z","timestamp":1760126609000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4426\/13\/7\/1072"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,29]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["jpm13071072"],"URL":"https:\/\/doi.org\/10.3390\/jpm13071072","relation":{},"ISSN":["2075-4426"],"issn-type":[{"value":"2075-4426","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,29]]}}}