{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:06:14Z","timestamp":1787313974639,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T00:00:00Z","timestamp":1690675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>To date, histological biomarkers expressed by laryngeal cancer are poorly known. The identification of biomarkers associated with laryngeal squamous cell carcinoma (SCC), would help explain the tumorogenesis and prevent the possible recurrence of the lesion after treatment. For this reason, the aim of this study is to investigate, for the first time, the Orphanin expression in 48 human specimens of laryngeal SCC and evaluate its possible correlation with patients prognosis. We analyzed pathological specimens from 48 patients with laryngeal SCC to detect the presence of Orphanin by using an immunohistochemistry test. We compared the findings with healthy tissue acquired from patients who underwent surgery for mesenchymal benign tumours of the larynx. The specimens were stained with anti-Orphanin monoclonal antibodies. Results were processed through a computerised image analysis system to determine a scale of staining intensity. All the tumoural specimens examined showed a significant immunoreaction for Orphanin when compared with healthy tissues (p &lt; 0.05) but with a different immune reactivity related to clinical-pathological features. A high Orphanin expression was not significantly related to Histological Grading (HG), TNM, and stage (p &gt; 0.05). In the multivariate analysis, the Orphanin expression was significantly related only to the malignant recurrence (p &lt; 0.05). Our study suggests that Orphanin could have a role in tumorigenesis by increasing the recurrence of cancer; therefore, it should be further explored as a possible biomarker for laryngeal cancer.<\/jats:p>","DOI":"10.3390\/jpm13081211","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T01:26:40Z","timestamp":1690766800000},"page":"1211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Prospective Observational Study on the Role of Immunohistochemical Expression of Orphanin in Laryngeal Squamous Cell Carcinoma Recurrence"],"prefix":"10.3390","volume":"13","author":[{"given":"Federico","family":"Sireci","sequence":"first","affiliation":[{"name":"Otorhinolaryngology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2120-5852","authenticated-orcid":false,"given":"Francesco","family":"Lorusso","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5844-9762","authenticated-orcid":false,"given":"Francesco","family":"Dispenza","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3414-6492","authenticated-orcid":false,"given":"Angelo","family":"Immordino","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Gallina","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Salvago","sequence":"additional","affiliation":[{"name":"Audiology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Martines","sequence":"additional","affiliation":[{"name":"Audiology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Bonaventura","sequence":"additional","affiliation":[{"name":"Histology and Embriology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Laura","family":"Uzzo","sequence":"additional","affiliation":[{"name":"Histology and Embriology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni Francesco","family":"Spatola","sequence":"additional","affiliation":[{"name":"Histology and Embriology Section, Biomedicine, Neuroscience and Advanced Diagnosics Department, University of Palermo, Via del Vespro 129, 133, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,30]]},"reference":[{"key":"ref_1","unstructured":"Barnes, L., Eveson, J., Reichart, P., and Sidransky, D. 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