{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:22:24Z","timestamp":1790619744516,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JPM"],"abstract":"<jats:p>Osteoarthritis (OA) is the most common complex musculoskeletal disorder, resulting from the degeneration of the articular cartilage and characterized by joint pain and dysfunction that culminate in progressive articular cartilage loss. We present our experience in the management of hip and knee OA by means of the intra-articular injection of fat micrograft, describing our approach, which was developed from the belief in the powerful reparative effect of autologous fat graft on damaged tissue, as well as its natural lubricating effect on the joints. Inclusion criteria were as follows: men and women, aged 20 to 80 years, that referred articular pain of the hips and\/or knees, showing initial-stage degenerative OA. From October 2018 to July 2023, a total of 250 patients underwent treatment with the Sefficare\u00ae device (SEFFILINE srl, Bologna, Italy). The Superficial Enhanced Fluid Fat Injection device was used to perform autologous regenerative treatments in a safe, standardized, easy, and effective way on 160 women, 64%, and 90 men, 36%. A total of 190 procedures (76%) involved the knees, with 20 patients who were bilaterally treated, while 60 procedures, all unilateral, involved the hips (24%). The mean age at treatment was 52.4 years. Before treatment, each patient had undergone X-rays and Magnetic Resonance Imaging (MRI) of the painful hip\/knee to evaluate and grade the articular OA. Postoperatively, each patient was assessed after one, three, six, and twelve months. The donor site postoperative course was uneventful other than minimal discomfort. Clinically, the ROM (range of motion) of the treated knee\/hip increased an average of 10 degrees 3 months after treatment, but the stiffness was reduced, as reported by the patients. The VAS (Visual Analog Scale) was submitted at 3, 6, and 12 months, demonstrating a progressive reduction of pain, with the best score obtained at six months postoperatively. In total, 85% of patients were satisfied one year after treatment, with a considerable improvement in pain and quality of life. The satisfactory outcome of this minimally invasive procedure indicates that the intra-articular injection of fat micrograft can replace or considerably delay the need for the classical major joint replacement surgery, thanks to its impact on the quality of life of patients and financial cost.<\/jats:p>","DOI":"10.3390\/jpm14060604","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T03:44:35Z","timestamp":1717645475000},"page":"604","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Role of Autologous Micro-Fragmented Adipose Tissue in Osteoarthritis Treatment"],"prefix":"10.3390","volume":"14","author":[{"given":"Paolo","family":"Trentani","sequence":"first","affiliation":[{"name":"Medipro Center Bologna, 40100 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Meredi","sequence":"additional","affiliation":[{"name":"Inail Insurance Medicine Center, 40100 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8135-0819","authenticated-orcid":false,"given":"Paola","family":"Zarantonello","sequence":"additional","affiliation":[{"name":"IRCCS Rizzoli Ortopaedic Institute Bologna-Argenta, 40100 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4105-725X","authenticated-orcid":false,"given":"Alessandro","family":"Gennai","sequence":"additional","affiliation":[{"name":"Studio Gennai, 40100 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.addr.2006.01.006","article-title":"Osteoarthritis","volume":"58","author":"Buckwalter","year":"2006","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/art.10913","article-title":"The costs of arthritis","volume":"49","author":"Dunlop","year":"2003","journal-title":"Arthritis Rheum."},{"key":"ref_3","unstructured":"Moshref, S.S., Jamal, Y.S., Al-Hibshi, A.M., and Kaki, A.M. (2019). Tibia Pathology and Fractures, IntechOpen."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"11795441211047284","DOI":"10.1177\/11795441211047284","article-title":"Intra-articular Hyaluronic Acid for Osteoarthritis of the Knee in the United States: A Systematic Review of Economic Evaluations","volume":"14","author":"Mordin","year":"2021","journal-title":"Clin. Med. Insights Arthritis Musculoskelet. Disord."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1089\/hum.2010.138","article-title":"Mesenchymal stem cells and osteoarthritis: Remedy or accomplice?","volume":"21","author":"Coleman","year":"2010","journal-title":"Hum. Gene Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/j.joca.2013.06.025","article-title":"Safety of intra-articular cell-therapy with culture-expanded stem cells in humans: A systematic literature review","volume":"21","author":"Peeters","year":"2013","journal-title":"Osteoarthr. Cartil."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1089\/107632701300062859","article-title":"Multilineage cells from human adipose tissue: Implications for cell-based therapies","volume":"7","author":"Zuk","year":"2001","journal-title":"Tissue Eng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4279","DOI":"10.1091\/mbc.e02-02-0105","article-title":"Human adipose tissue is a source of multipotent stem cells","volume":"13","author":"Zuk","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1091\/mbc.e09-07-0589","article-title":"The adipose-derived stem cell: Looking back and looking ahead","volume":"21","author":"Zuk","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s12265-011-9257-3","article-title":"Adult human adipose tissue contains several types of multipotent cells","volume":"4","author":"Tallone","year":"2011","journal-title":"J. Cardiovasc. Transl. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1097\/00006534-200203000-00037","article-title":"Rat extramedullary adipose tissue as a source of osteochondrogenic progenitor cells","volume":"109","author":"Huang","year":"2002","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S33","DOI":"10.1038\/ncpcardio0444","article-title":"Plasticity of human adipose stem cells toward endothelial cells and cardiomyocytes","volume":"3","author":"Fraser","year":"2006","journal-title":"Nat. Clin. Pract. Cardiovasc. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1002\/jcb.20886","article-title":"Mesenchymal stem cells as trophic mediators","volume":"98","author":"Caplan","year":"2006","journal-title":"J. Cell Biochem."},{"key":"ref_14","first-page":"13","article-title":"Differentiation of normal and dysplastic juvenile hip joints by means of the summarized hip factor","volume":"74","author":"Hepp","year":"1971","journal-title":"Arch Orthop Unfallchir."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1136\/ard.16.4.494","article-title":"Radiological assessment of osteo-arthrosis","volume":"16","author":"Kellgren","year":"1957","journal-title":"Ann. Rheum. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1097\/01.blo.0000534679.75870.5f","article-title":"Classifications in Brief: T\u00f6nnis Classification of Hip Osteoarthritis","volume":"476","author":"Kovalenko","year":"2018","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1007\/s11999-016-4732-4","article-title":"Classifications in Brief: Kellgren-Lawrence Classification of Osteoarthritis","volume":"474","author":"Kohn","year":"2016","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1046\/j.1365-2702.2001.00525.x","article-title":"Examining the validity of pressure ulcer risk assessment scales: Developing and using illustrated patient simulations to collect the data INFORMATION POINT: Visual Analogue Scale","volume":"10","author":"Gould","year":"2001","journal-title":"J. Clin. Nurs."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1177\/1090820X14528000","article-title":"Harvest of superficial layers of fat with a microcannula and isolation of adipose tissue-derived stromal and vascular cells","volume":"34","author":"Trivisonno","year":"2014","journal-title":"Aesthet.Surg. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.jcyt.2015.04.004","article-title":"Qualitative and quantitative differences of adipose- derived stromal cells from superficial and deep subcutaneous lipoaspirates: A matter of fat","volume":"17","author":"Cicione","year":"2015","journal-title":"Cytotherapy"},{"key":"ref_21","first-page":"e2239","article-title":"Superficial enhanced fluid fat injection (SEFFI and MicroSEFFI) in facial rejuvenation","volume":"5","author":"Gennai","year":"2017","journal-title":"CellR4"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1093\/asj\/sjv001","article-title":"Superficial Enhanced Fluid Fat Injection (SEFFI) to correct volume defects and skin aging of the face and periocular region","volume":"35","author":"Bernardini","year":"2015","journal-title":"Aesthet. Surg. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1001\/jamafacial.2015.1146","article-title":"Fluid fat injection for volume restoration and skin regeneration of the periocular aesthetic unit","volume":"18","author":"Bernardini","year":"2016","journal-title":"JAMA Facial Plast. Surg."},{"key":"ref_24","first-page":"2","article-title":"Comparison of Harvesting and Processing Technique for Adipose Tissue Graft: Evaluation of Cell Viability","volume":"4","author":"Gennai","year":"2021","journal-title":"Int. J. Regenr. Med."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Senesi, L., De Francesco, F., Farinelli, L., Manzotti, S., Gagliardi, G., Papalia, G.F., Riccio, M., and Gigante, A. (2019). Mechanical and Enzymatic Procedures to Isolate the Stromal Vascular Fraction from Adipose Tissue: Preliminary Results. Front. Cell Dev. Biol., 7.","DOI":"10.3389\/fcell.2019.00088"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1093\/asj\/sjw106","article-title":"The Effects of Fat Harvesting and Preparation, Air Exposure, Obesity, and Stem Cell Enrichment on Adipocyte Viability Prior to Graft Transplantation","volume":"35","author":"Cucchiani","year":"2016","journal-title":"Aesthetic Surg. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1097\/00005650-199603000-00003","article-title":"A 12-Item Short-Form Health Survey: Construction of scales and preliminary tests of reliability and validity","volume":"34","author":"Ware","year":"1996","journal-title":"Med. Care"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/S0895-4356(98)00109-7","article-title":"Cross-validation of item selection and scoring for the SF-12 Health Survey in nine countries: Results from the IQOLA Project","volume":"51","author":"Gandek","year":"1998","journal-title":"J. Clin. Epidemiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/oxfordjournals.pubmed.a024606","article-title":"A shorter form health survey: Can the SF-12 replicate results from the SF-36 in longitudinal studies?","volume":"19","author":"Jenkinson","year":"1997","journal-title":"J. Public Health"},{"key":"ref_30","unstructured":"Ware, J.E., Keller, S.D., and Kosinski, M. (1995). SF-12: How to Score the SF-12 Physical and Mental Health Summary Scales, Health Institute, New England Medical Center."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.stem.2008.07.003","article-title":"A perivascular origin for mesenchymal stem cells in multiple human organs","volume":"3","author":"Crisan","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Thoene, M., Bejer-Olenska, E., and Wojtkiewicz, J. (2023). The Current State of Osteoarthritis Treatment Options Using Stem Cells for Regenerative Therapy: A Review. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24108925"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"101684","DOI":"10.1016\/j.arr.2022.101684","article-title":"Research progress in the use of mesenchymal stem cells and their derived exosomes in the treatment of osteoarthritis","volume":"80","author":"Yu","year":"2022","journal-title":"Ageing Res. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Giorgino, R., Albano, D., Fusco, S., Peretti, G.M., Mangiavini, L., and Messina, C. (2023). Knee Osteoarthritis: Epidemiology, Pathogenesis, and Mesenchymal Stem Cells: What Else Is New? An Update. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24076405"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1002\/sctm.18-0122","article-title":"Intra-Articular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis: A Phase IIb, Randomized, Placebo-Controlled Clinical Trial","volume":"8","author":"Lee","year":"2019","journal-title":"Stem Cells Transl. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"295","DOI":"10.2217\/rme-2017-0152","article-title":"Human adipose-derived mesenchymal stem cells for osteoarthritis: A pilot study with long-term follow-up and repeated injections","volume":"13","author":"Song","year":"2018","journal-title":"Regen. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"749","DOI":"10.2217\/rme-2023-0071","article-title":"Current evidence on mesenchymal stem cells for hip osteoarthritis: A narrative review","volume":"18","author":"Entessari","year":"2023","journal-title":"Regen. Med."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wei, P., and Bao, R. (2022). Intra-Articular Mesenchymal Stem Cell Injection for Knee Osteoarthritis: Mechanisms and Clinical Evidence. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010059"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1756-185X.2011.01599.x","article-title":"Mesenchymal stem cell therapy for knee osteoarthritis. Preliminary report of four patients","volume":"14","author":"Davatchi","year":"2011","journal-title":"Int. J. Rheum. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"213","DOI":"10.2217\/rme-2018-0161","article-title":"Adipose-derived mesenchymal stem cell therapy in the treatment of knee osteoarthritis: A randomized controlled trial","volume":"14","author":"Freitag","year":"2019","journal-title":"Regen. Med."}],"container-title":["Journal of Personalized Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4426\/14\/6\/604\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:54:26Z","timestamp":1760108066000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4426\/14\/6\/604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,6]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["jpm14060604"],"URL":"https:\/\/doi.org\/10.3390\/jpm14060604","relation":{},"ISSN":["2075-4426"],"issn-type":[{"value":"2075-4426","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,6]]}}}