{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:02:00Z","timestamp":1791849720715,"version":"4.3.4"},"reference-count":24,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["JPM"],"abstract":"<jats:p>Background\/Objectives: Synovial sarcoma (SS) is an aggressive soft-tissue tumor characterized by the chromosomal translocation t(X;18) (p11.2;q11.2), most commonly involving the fusion of the SYT gene on chromosome 18 with the SSX1 or SSX2 genes on chromosome X. This study aims to explore the clinicopathological and molecular characteristics of synovial sarcoma in a cohort of Moroccan patients. Methods: We analyzed 48 cases of synovial sarcoma using formalin-fixed, paraffin-embedded (FFPE) tissue samples. Histological grading was performed according to the FNCLCC system. Immunohistochemical staining was employed to detect cytokeratin (CK) and epithelial membrane antigen (EMA). Molecular analysis included fluorescence in situ hybridization (FISH) to identify SS18 gene rearrangements and reverse transcription\u2013polymerase chain reaction (RT-PCR) to detect SYT-SSX fusion transcripts. Results: Among the cohort, 56% of cases showed SS18 gene rearrangements via FISH, while RT-PCR confirmed the presence of SS18-SSX1 and SS18-SSX2 transcripts in 60% and 32% of cases, respectively. The remainder was classified as undifferentiated sarcoma. Notably, no significant associations were observed between SYT-SSX fusion type and clinicopathological features. Conclusions: These findings underscore the importance of integrating molecular techniques for precise diagnosis in synovial sarcoma. The results align with global patterns, emphasizing the necessity for molecular testing to enhance diagnostic accuracy and informing potential therapeutic advancements.<\/jats:p>","DOI":"10.3390\/jpm15100455","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T14:01:39Z","timestamp":1759154499000},"page":"455","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Profiling of SYT-SSX Fusion Transcripts for Enhanced Diagnosis of Synovial Sarcomas"],"prefix":"10.3390","volume":"15","author":[{"given":"Sara","family":"Louati","sequence":"first","affiliation":[{"name":"Medical Biotechnology Laboratory, Medical and Pharmacy School, Mohammed V University of Rabat, Rabat BP 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoutar","family":"Bentayebi","sequence":"additional","affiliation":[{"name":"Medical Biotechnology Laboratory, Medical and Pharmacy School, Mohammed V University of Rabat, Rabat BP 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5478-2123","authenticated-orcid":false,"given":"Ibtissam","family":"Saad","sequence":"additional","affiliation":[{"name":"Mohammed VI University of Sciences and Health (UM6SS), Casablanca 20000, Morocco"},{"name":"Mohammed VI Center for Research and Innovation (CM6RI), Rabat 10112, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8790-0479","authenticated-orcid":false,"given":"Yvonne","family":"Gloor","sequence":"additional","affiliation":[{"name":"CANSEARCH Research Platform for Pediatric Oncology and Hematology, Department of Pediatrics, Gynecology and Obstetrics, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7747-7454","authenticated-orcid":false,"given":"Nadia","family":"Senhaji","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, Moulay Ismail University of Meknes, Meknes 50000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelmajid","family":"Elmrini","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery B4, CHU Hassan II Hospital, University of Sidi Mohammed Ben Abdellah, Fez 30000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lahcen","family":"Belyamani","sequence":"additional","affiliation":[{"name":"Mohammed VI University of Sciences and Health (UM6SS), Casablanca 20000, Morocco"},{"name":"Emergency Department, Military Hospital Mohammed V, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachid","family":"Eljaoudi","sequence":"additional","affiliation":[{"name":"Medical Biotechnology Laboratory, Medical and Pharmacy School, Mohammed V University of Rabat, Rabat BP 10000, Morocco"},{"name":"Pharmacology and Toxicology Department, Medical and Pharmacy School, Mohammed V University in Rabat, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9649-6498","authenticated-orcid":false,"given":"Marc","family":"Ansari","sequence":"additional","affiliation":[{"name":"CANSEARCH Research Platform for Pediatric Oncology and Hematology, Department of Pediatrics, Gynecology and Obstetrics, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland"},{"name":"Division of Pediatric Oncology and Hematology, Department of Women, Child, and Adolescent, University Hospital of Geneva, 1205 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6405-8322","authenticated-orcid":false,"given":"Sanae","family":"Bennis","sequence":"additional","affiliation":[{"name":"Laboratory of Biomedical and Translational Research, Faculty of Medicine, Pharmacy and Dental Medicine of Fez, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-9383","authenticated-orcid":false,"given":"Youssef","family":"Daali","sequence":"additional","affiliation":[{"name":"Division of Clinical Pharmacology and Toxicology, Department of Anesthesiology, Pharmacology, Intensive Care and Emergency Medicine, Geneva University Hospitals, 1205 Geneva, Switzerland"},{"name":"Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland"},{"name":"School of Pharmaceutical Sciences, University of Geneva, 1205 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.21037\/tp-24-59","article-title":"Epidemiology, incidence, and survival of synovial sarcoma of children: A SEER database analysis","volume":"13","author":"Zhu","year":"2024","journal-title":"Transl. 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