{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:52:53Z","timestamp":1787028773863,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T00:00:00Z","timestamp":1615507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BE3703\/8-2"],"award-info":[{"award-number":["BE3703\/8-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Background: Feather pecking is a well-known problem in layer flocks that causes animal welfare restrictions and contributes to economic losses. Birds\u2019 gut microbiota has been linked to feather pecking. This study aims to characterize the microbial communities of two laying hen lines divergently selected for high (HFP) and low (LFP) feather pecking and investigates if the microbiota is associated with feather pecking or agonistic behavior. Methods: Besides phenotyping for the behavioral traits, microbial communities from the digesta and mucosa of the ileum and caeca were investigated using target amplicon sequencing and functional predictions. Microbiability was estimated with a microbial mixed linear model. Results: Ileum digesta showed an increase in the abundance of the genus Lactobacillus in LFP, while Escherichia was abundant in HFP hens. In the caeca digesta and mucosa of the LFP line were more abundant Faecalibacterium and Blautia. Tryptophan metabolism and lysine degradation were higher in both digesta and mucosa of the HFP hens. Linear models revealed that the two lines differ significantly in all behavior traits. Microbiabilities were close to zero and not significant in both lines and for all traits. Conclusions: Trait variation was not affected by the gut microbial composition in both selection lines.<\/jats:p>","DOI":"10.3390\/life11030235","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T22:13:10Z","timestamp":1615759990000},"page":"235","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Gut Microbial Composition and Predicted Functions Are Not Associated with Feather Pecking and Antagonistic Behavior in Laying Hens"],"prefix":"10.3390","volume":"11","author":[{"given":"Daniel","family":"Borda-Molina","sequence":"first","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Iffland","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Schmid","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svenja","family":"Schad","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7690-8539","authenticated-orcid":false,"given":"Jana","family":"Seifert","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5352-464X","authenticated-orcid":false,"given":"Jens","family":"Tetens","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of G\u00f6ttingen, 37073 G\u00f6ttingen, Germany"},{"name":"Center for Integrated Breeding Research, University of G\u00f6ttingen, 37075 G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Bessei","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rn","family":"Bennewitz","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7814-6569","authenticated-orcid":false,"given":"Am\u00e9lia","family":"Camarinha-Silva","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1017\/S0043933913000354","article-title":"The prevention and control of feather pecking in laying hens: Identifying the underlying principles","volume":"69","author":"Rodenburg","year":"2013","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.applanim.2004.05.004","article-title":"Chronic increase of dietary l-tryptophan decreases gentle feather pecking behaviour","volume":"89","author":"Koolhaas","year":"2004","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1080\/1828051X.2017.1340814","article-title":"Gut microbiota and transportation stress response affected by tryptophan supplementation in broiler chickens","volume":"17","author":"Bello","year":"2018","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0168-1591(99)00051-9","article-title":"Feather eating in layer pullets and its possible role in the aetiology of feather pecking damage","volume":"65","author":"McKeegan","year":"1999","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0168-1591(01)00124-1","article-title":"Feather eating in individually caged hens which differ in their prospensity to feather peck","volume":"73","author":"McKeegan","year":"2001","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_6","first-page":"241","article-title":"Feather eating and crop filling in laying hens","volume":"69","author":"Bessei","year":"2005","journal-title":"Archiv. Gefl\u00fcgelkunde"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.applanim.2008.11.003","article-title":"Understanding feather eating behaviour in laying hens","volume":"117","year":"2009","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.3382\/ps.0451231","article-title":"Methods for Determining the Nutritive Value of Feather Meals","volume":"45","author":"McCasland","year":"1966","journal-title":"Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.3382\/ps\/pew146","article-title":"Quantitative genetic analysis of causal relationships among feather pecking, feather eating, and general locomotor activity in laying hens using structural equation models","volume":"95","author":"Lutz","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.3382\/ps.2011-01786","article-title":"Dietary inclusion of feathers affects intestinal microbiota and microbial metabolites in growing Leghorn-type chickens","volume":"91","author":"Meyer","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.physbeh.2012.12.017","article-title":"Differences in intestinal microbial metabolites in laying hens with high and low levels of repetitive feather-pecking behavior","volume":"110\u2013111","author":"Meyer","year":"2013","journal-title":"Physiol. Behav."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3009","DOI":"10.3382\/ps\/pey167","article-title":"Differences in cecal microbiome of selected high and low feather-pecking laying hens","volume":"97","author":"Birkl","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7009","DOI":"10.3382\/ps\/pez336","article-title":"Differences in gut microbiota composition of laying hen lines divergently selected on feather pecking","volume":"98","author":"Kjaer","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6816","DOI":"10.1128\/AEM.69.11.6816-6824.2003","article-title":"Diversity and succession of the intestinal bacterial community of the maturing broiler chicken","volume":"69","author":"Lu","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_15","first-page":"2033","article-title":"Insights into Broilers\u2019 Gut Microbiota Fed with Phosphorus, Calcium, and Phytase Supplemented Diets","volume":"7","author":"Vital","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1007\/s00253-014-5646-2","article-title":"Microbiota of the chicken gastrointestinal tract: Influence on health, productivity and disease","volume":"98","author":"Stanley","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4161\/gmic.26945","article-title":"Intestinal microbiome of poultry and its interaction with host and diet","volume":"5","author":"Pan","year":"2014","journal-title":"Gut Microbes"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/1574-6968.12608","article-title":"The chicken gastrointestinal microbiome","volume":"360","author":"Oakley","year":"2014","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.csbj.2014.12.005","article-title":"News in livestock research\u2014Use of Omics-technologies to study the microbiota in the gastrointestinal tract of farm animals","volume":"13","author":"Deusch","year":"2015","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"673","DOI":"10.3389\/fmicb.2015.00673","article-title":"Exploring the avian gut microbiota: Current trends and future directions","volume":"6","author":"Waite","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Difford, G.F., Plichta, D.R., L\u00f8vendahl, P., Lassen, J., Noel, S.J., H\u00f8jberg, O., Wright, A.-D.G., Zhu, Z., Kristensen, L., and Nielsen, H.B. (2018). Host genetics and the rumen microbiome jointly associate with methane emissions in dairy cows. PLoS Genet., 14.","DOI":"10.1371\/journal.pgen.1007580"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1534\/genetics.117.200782","article-title":"Host Genome Influence on Gut Microbial Composition and Microbial Prediction of Complex Traits in Pigs","volume":"206","author":"Maushammer","year":"2017","journal-title":"Genetics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/jbg.12447","article-title":"Selecting the hologenome to breed for an improved feed efficiency in pigs-A novel selection index","volume":"137","author":"Weishaar","year":"2020","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/jbg.12433","article-title":"Prediction of nutrient digestibility in grower-finisher pigs based on faecal microbiota composition","volume":"137","author":"Verschuren","year":"2020","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1534\/g3.120.401424","article-title":"The Gut Microbial Architecture of Efficiency Traits in the Domestic Poultry Model Species Japanese Quail (Coturnix japonica) Assessed by Mixed Linear Models","volume":"10","author":"Vollmar","year":"2020","journal-title":"G3"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"112658","DOI":"10.1016\/j.physbeh.2019.112658","article-title":"Influence of the microbiota-gut-brain axis on behavior and welfare in farm animals: A review","volume":"210","author":"Kraimi","year":"2019","journal-title":"Physiol. Behav."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"jeb202879","DOI":"10.1242\/jeb.202879","article-title":"Effects of gut microbiota transfer on emotional reactivity in Japanese quails (Coturnix japonica)","volume":"222","author":"Kraimi","year":"2019","journal-title":"J. Exp. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.bbr.2017.05.022","article-title":"The influence of a probiotic supplementation on memory in quail suggests a role of gut microbiota on cognitive abilities in birds","volume":"331","author":"Parois","year":"2017","journal-title":"Behav. Brain Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Iffland, H., Wellmann, R., Schmid, M., Preu\u00df, S., Tetens, J., Bessei, W., and Bennewitz, J. (2020). Genomewide Mapping of Selection Signatures and Genes for Extreme Feather Pecking in Two Divergently Selected Laying Hen Lines. Animals, 10.","DOI":"10.3390\/ani10020262"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105177","DOI":"10.1016\/j.applanim.2020.105177","article-title":"Phenotypic and genomic analyses of agonistic interactions in laying hen lines divergently selected for feather pecking","volume":"234","author":"Iffland","year":"2021","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40104-017-0168-2","article-title":"Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs","volume":"8","author":"Kaewtapee","year":"2017","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7788","DOI":"10.1038\/s41598-018-26083-3","article-title":"Early Salmonella Typhimurium infection in pigs disrupts Microbiome composition and functionality principally at the ileum mucosa","volume":"8","author":"Carvajal","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Borda-Molina, D., Roth, C., H\u00e9rnandez-Arriaga, A., Rissi, D., Vollmar, S., Rodehutscord, M., Bennewitz, J., and Camarinha-Silva, A. (2020). Effects on the Ileal Microbiota of Phosphorus and Calcium Utilization, Bird Performance, and Gender in Japanese Quail. Animals, 10.","DOI":"10.3390\/ani10050885"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/bioinformatics\/btr381","article-title":"UCHIME improves sensitivity and speed of chimera detection","volume":"27","author":"Edgar","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40793-020-00358-7","article-title":"Tax4Fun2: Prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences","volume":"15","author":"Wemheuer","year":"2020","journal-title":"Environ. Microbiome"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D643","DOI":"10.1093\/nar\/gkt1209","article-title":"The SILVA and \u201cAll-species Living Tree Project (LTP)\u201d taxonomic frameworks","volume":"42","author":"Yilmaz","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a reference resource for gene and protein annotation","volume":"44","author":"Kanehisa","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/s41596-019-0264-1","article-title":"Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data","volume":"15","author":"Chong","year":"2020","journal-title":"Nat. Protoc."},{"key":"ref_41","unstructured":"Clarke, K.R., and Warwick, R.M. (1994). Change in Marine Communities: An Approach to Statistical Analysis and Interpretation, PRIMER-E. [2nd ed.]."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"325","DOI":"10.2307\/1942268","article-title":"An Ordination of the Upland Forest Communities of Southern Wisconsin","volume":"27","author":"Bray","year":"1957","journal-title":"Ecol. Monogr."},{"key":"ref_43","unstructured":"Butler, D., Cullis, B.R., Gilmour, A.R., and Gogel, B.J. (2009). ASReml-R 3 Reference Manual: Mixed Models for S Language Environments."},{"key":"ref_44","unstructured":"R Core Team (2019). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/s40168-018-0568-3","article-title":"Fecal source identification using random forest","volume":"6","author":"Roguet","year":"2018","journal-title":"Microbiome"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1079\/NRR200479","article-title":"Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics","volume":"17","author":"Gibson","year":"2004","journal-title":"Nutr. Res. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"S160","DOI":"10.1097\/MCG.0b013e31817da140","article-title":"Functional genomics of probiotic Lactobacilli","volume":"42","author":"Klaenhammer","year":"2008","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.3382\/ps.2012-02168","article-title":"Evaluating the efficacy of an avian-specific probiotic to reduce the colonization of Campylobacter jejuni in broiler chickens","volume":"91","author":"Ghareeb","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1472-765X.2004.01474.x","article-title":"In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry","volume":"38","author":"Narbad","year":"2004","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4981","DOI":"10.1128\/AEM.65.11.4981-4986.1999","article-title":"Lactobacillus salivarius CTC2197 prevents Salmonella enteritidis colonization in chickens","volume":"65","author":"Pascual","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.3382\/ps.2009-00041","article-title":"Screening of lactic acid bacteria toward their selection as a source of chicken probiotic","volume":"88","author":"Taheri","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.neulet.2013.01.037","article-title":"Effects of intraduodenal injection of Lactobacillus brevis SBC8803 on autonomic neurotransmission and appetite in rodents","volume":"539","author":"Horii","year":"2013","journal-title":"Neurosci Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.bbr.2015.10.046","article-title":"Alteration of behavior and monoamine levels attributable to Lactobacillus plantarum PS128 in germ-free mice","volume":"298","author":"Liu","year":"2016","journal-title":"Behav. Brain Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"16050","DOI":"10.1073\/pnas.1102999108","article-title":"Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve","volume":"108","author":"Bravo","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.neuroscience.2015.09.033","article-title":"Administration of Lactobacillus helveticus NS8 improves behavioral, cognitive, and biochemical aberrations caused by chronic restraint stress","volume":"310","author":"Liang","year":"2015","journal-title":"Neuroscience"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1099\/ijsem.0.003012","article-title":"Romboutsia hominis sp. nov., the first human gut-derived representative of the genus Romboutsia, isolated from ileostoma effluent","volume":"68","author":"Gerritsen","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Belzer, C., Gerber, G.K., Roeselers, G., Delaney, M., DuBois, A., Liu, Q., Belavusava, V., Yeliseyev, V., Houseman, A., and Onderdonk, A. (2014). Dynamics of the microbiota in response to host infection. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0095534"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1128\/AEM.01226-07","article-title":"The Mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract","volume":"74","author":"Derrien","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1080\/19490976.2015.1086057","article-title":"The colonic mucus protection depends on the microbiota","volume":"6","author":"Johansson","year":"2015","journal-title":"Gut Microbes"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.neubiorev.2018.07.007","article-title":"Where in the serotonergic system does it go wrong? Unravelling the route by which the serotonergic system affects feather pecking in chickens","volume":"95","year":"2018","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s12711-015-0154-0","article-title":"Genetic parameters and signatures of selection in two divergent laying hen lines selected for feather pecking behaviour","volume":"47","author":"Grams","year":"2015","journal-title":"Genet Sel. Evol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nrmicro2540","article-title":"Unravelling the effects of the environment and host genotype on the gut microbiome","volume":"9","author":"Spor","year":"2011","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/11\/3\/235\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:35:01Z","timestamp":1760160901000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/11\/3\/235"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,12]]},"references-count":62,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["life11030235"],"URL":"https:\/\/doi.org\/10.3390\/life11030235","relation":{},"ISSN":["2075-1729"],"issn-type":[{"value":"2075-1729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,12]]}}}