{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:03:20Z","timestamp":1790960600149,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T00:00:00Z","timestamp":1619136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC-101-2314-B-010-030-MY3"],"award-info":[{"award-number":["NSC-101-2314-B-010-030-MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Adult-onset Still\u2019s disease (AOSD), an autoinflammatory disorder, is related to the dysregulation of NLR3-containing a pyrin domain (NLRP3)-inflammasome signaling. We aimed to investigate the associations of genetic polymorphisms of NLRP3-inflammasome signaling with AOSD susceptibility and outcome and to examine their functional property. Fifty-three candidate single-nucleotide polymorphisms (SNPs) involved in NLRP3-inflammasome response were genotyped using Sequenom MassArray on the samples from 66 AOSD patients and 128 healthy controls. The significant SNPs were validated by direct sequencing using a TaqMan SNP analyzer. Serum levels of associated gene products were examined by ELISA. One SNP rs11672725 of CARD8 gene was identified to be significantly associated with AOSD susceptibility by using MassArray and subsequent replication validation (p = 3.57 \u00d7 10\u22127; odds ratio 3.02). Functional assays showed that serum CARD8 levels were significantly lower in AOSD patients (median, 10,524.6 pg\/mL) compared to controls (13,964.1 pg\/mL, p = 0.005), while levels of caspase-1, IL-1\u03b2 and IL-18 were significantly higher in patients (107.1 pg\/mL, 2.1 pg\/mL, and 1495.8 pg\/mL, respectively) than those in controls (99.0 pg\/mL, 1.0 pg\/mL, and 141.4 pg\/mL, respectively). Patients carrying rs11672725CC genotype had significantly higher serum caspase-1 and IL-18 levels (121.3 pg\/mL and 1748.6 pg\/mL) compared to those with CT\/TT genotypes (72.6 pg\/mL, p = 0.019 and 609.3 pg\/mL, p = 0.046). A higher proportion of patients with rs11672725CC genotype had a systemic pattern of disease outcome, which was linked to low CARD8 levels. A novel variant, rs11672725, of the CARD8 gene was identified as a potential genetic risk for AOSD. Patients carrying the rs11672725CC genotype and C allele had low CARD8 levels, and were predisposed to a systemic pattern with an elevated expression of inflammasome signaling.<\/jats:p>","DOI":"10.3390\/life11050382","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T12:08:30Z","timestamp":1619179710000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["CARD8 SNP rs11672725 Identified as a Potential Genetic Variant for Adult-Onset Still\u2019s Disease"],"prefix":"10.3390","volume":"11","author":[{"given":"Wei-Ting","family":"Hung","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan"},{"name":"Department of Medical Education, Taichung Veterans General Hospital, Taichung 40705, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7593-3065","authenticated-orcid":false,"given":"Yi-Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan"},{"name":"School of Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan"},{"name":"Rong Hsing Research Center for Translational Medicine &amp; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6531-5538","authenticated-orcid":false,"given":"Shuen-Iu","family":"Hung","sequence":"additional","affiliation":[{"name":"Cancer Vaccine and Immune Cell Therapy Core Laboratory, Chang Gung Immunology Consortium, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan"},{"name":"School of Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan"},{"name":"Rong Hsing Research Center for Translational Medicine &amp; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5097-7376","authenticated-orcid":false,"given":"Ning-Rong","family":"Gung","sequence":"additional","affiliation":[{"name":"Rheumatology and Immunology Center, Translational Medicine Laboratory, China Medical University Hospital, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Wei","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Rong Hsing Research Center for Translational Medicine &amp; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung 40705, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Tung","family":"Tang","sequence":"additional","affiliation":[{"name":"Rong Hsing Research Center for Translational Medicine &amp; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung 40705, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Der-Yuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Rheumatology and Immunology Center, Translational Medicine Laboratory, China Medical University Hospital, Taichung 40447, Taiwan"},{"name":"Rheumatology and Immunology Center, China Medical University Hospital, Taichung 40447, Taiwan"},{"name":"School of Medicine, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1136\/ard.30.2.121","article-title":"Still\u2019s disease of the adults","volume":"30","author":"Bywaters","year":"1971","journal-title":"Ann. 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