{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:08:22Z","timestamp":1791846502311,"version":"4.3.4"},"reference-count":30,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Skin ageing has many manifestations such as wrinkles, dryness, hyperpigmentation, and uneven skin tone. Extrinsic and intrinsic factors, especially solar ultraviolet light (UVB), contribute to skin ageing; its main features are brown spots, alterations in melanin pigmentation, and a decrease in collagen and hyaluronic acid linked to oxidative stress. Several studies showed that topical products containing ingredients with antioxidant activity can reduce oxidative damage; to provide a maximum anti-ageing effect to the skin, topical products can combine various ingredients. C-SHOT SERUM contains a combination of two molecules with a proven anti-ageing activity: a high percentage (30%) of a more stable vitamin C derivative, 3-O-ethyl-l-ascorbic acid, and lactic acid (1%). The product showed a high biocompatibility, assessed through an MTT assay on keratinocytes and on Reconstructed Human Epidermis (RHE, SkinEthic); the anti-ageing activity was demonstrated on human dermal fibroblasts and keratinocytes by a statistically significant increase in collagen production and a reduction of a UVB-induced DNA damage marker (\u03b3-H2AX histone), indicating DNA protection. Moreover, a depigmenting activity, shown by a highly significant decrease in melanin content on treated Reconstructed Human Pigmented Epidermis (RHPE), was assessed. According to the data of our study, the tested product contrasts the effect of skin ageing and irregular pigmentation due to the physiological decline of the skin.<\/jats:p>","DOI":"10.3390\/life11050406","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T04:30:11Z","timestamp":1619670611000},"page":"406","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Anti-Ageing and Whitening Potential of a Cosmetic Serum Containing 3-O-ethyl-l-ascorbic Acid"],"prefix":"10.3390","volume":"11","author":[{"given":"Nicola","family":"Zerbinati","sequence":"first","affiliation":[{"name":"Department of Medicine and Surgery, University of Insubria, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Sommatis","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Maccario","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Di Francesco","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0459-155X","authenticated-orcid":false,"given":"Maria Chiara","family":"Capillo","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4497-3563","authenticated-orcid":false,"given":"Raffaele","family":"Rauso","sequence":"additional","affiliation":[{"name":"Maxillofacial Surgery Unit, University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Herrera","sequence":"additional","affiliation":[{"name":"Centro Avanzado de Dermatologia y Laser, San Pedro Sula 34101, Honduras"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pier Luca","family":"Bencini","sequence":"additional","affiliation":[{"name":"Istituto di Chirurgia e Laser-Chirurgia in Dermatologia (I.C.L.I.D.), 20121 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Guida","sequence":"additional","affiliation":[{"name":"Dermatology Unit, Department of Surgical, Medical, Dental and Morphological Sciences Related to Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, 41124 Modena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Mocchi","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3892\/ijmm.2016.2807","article-title":"Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-\u03b2\/Smad signaling pathway in the human dermal fbroblast cell line Hs27","volume":"39","year":"2017","journal-title":"Int. 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