{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:25:03Z","timestamp":1760235903986,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T00:00:00Z","timestamp":1634083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Junior Grant of Palacky University Olomouc","award":["JG_2019_005"],"award-info":[{"award-number":["JG_2019_005"]}]},{"name":"Increasing Internationalization of the Faculty of Medicine and Dentistry, Palacky University Olomouc","award":["SPP 210015017"],"award-info":[{"award-number":["SPP 210015017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter baumannii, is becoming a serious threat to public health. It is necessary to detect \u03b2-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of \u03b2-lactamases in a selected clinical isolate of A. baumannii of ST2P\/ST195Ox and to characterize possible enzymes, as well as its \u03b2-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing confirmed that the isolate harbored five bla gene alleles, namely, blaADC-73, blaTEM-1, blaOXA-23, blaOXA-58 and blaOXA-66, as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and\/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to \u03b2-lactams in this A. baumannii isolate is mainly due to an overproduction of \u03b2-lactamases in combination with other resistance mechanism (efflux pump system).<\/jats:p>","DOI":"10.3390\/life11101079","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T21:36:15Z","timestamp":1634160975000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic"],"prefix":"10.3390","volume":"11","author":[{"given":"Patrik","family":"Mlynarcik","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Dolejska","sequence":"additional","affiliation":[{"name":"Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic"},{"name":"CEITEC VETUNI Brno, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic"},{"name":"Department of Clinical Microbiology and Immunology, Institute of Laboratory Medicine, The University Hospital Brno, 62500 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Vagnerova","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515 Olomouc, Czech Republic"},{"name":"University Hospital Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Petrzelova","sequence":"additional","affiliation":[{"name":"University Hospital Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Sukkar","sequence":"additional","affiliation":[{"name":"CEITEC VETUNI Brno, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Zdarska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4621-8868","authenticated-orcid":false,"given":"Milan","family":"Kolar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77515 Olomouc, Czech Republic"},{"name":"University Hospital Olomouc, 77900 Olomouc, Czech Republic"},{"name":"Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77900 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s40121-020-00395-2","article-title":"Continuous Evolution: Perspective on the Epidemiology of Carbapenemase Resistance Among Enterobacterales and Other Gram-Negative Bacteria","volume":"10","author":"Hansen","year":"2021","journal-title":"Infect. Dis. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.amjmed.2006.03.011","article-title":"Mechanisms of antimicrobial resistance in bacteria","volume":"119","author":"Tenover","year":"2006","journal-title":"Am. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1128\/CMR.00058-07","article-title":"Acinetobacter baumannii: Emergence of a successful pathogen","volume":"21","author":"Peleg","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8053","DOI":"10.1128\/JB.00834-08","article-title":"Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii","volume":"190","author":"Adams","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_5","unstructured":"ECDC (2019). European Centre for Disease Prevention and Control. Surveillance of Antimicrobial Resistance in Europe 2018, ECDC. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/surveillance-antimicrobial-resistance-europe-2018."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3014","DOI":"10.1093\/jac\/dkv213","article-title":"Evaluation of the RAPIDEC (R) CARBA NP, the Rapid CARB Screen (R) and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae","volume":"70","author":"Dortet","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"141","DOI":"10.5507\/bp.2018.037","article-title":"Identification of novel OXA-134-like beta-lactamases in Acinetobacter lwoffii and Acinetobacter schindleri isolated from chicken litter","volume":"163","author":"Mlynarcik","year":"2019","journal-title":"Biomed. Pap. Med. Fac. Univ. Palacky Olomouc. Czech. Repub."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"fnab068","DOI":"10.1093\/femsle\/fnab068","article-title":"Detection of clinically important beta-lactamases by using PCR","volume":"368","author":"Mlynarcik","year":"2021","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Kolar, M., Htoutou Sedlakova, M., Urbanek, K., Mlynarcik, P., Roderova, M., Hricova, K., Mezerova, K., Kucova, P., Zapletalova, J., and Fiserova, K. (2021). Implementation of Antibiotic Stewardship in a University Hospital Setting. Antibiotics, 10.","DOI":"10.3390\/antibiotics10010093"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3389\/fcimb.2017.00055","article-title":"Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options","volume":"7","author":"Lee","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1089\/mdr.2019.0330","article-title":"PCR Detection of Oxacillinases in Bacteria","volume":"26","author":"Mlynarcik","year":"2020","journal-title":"Microb. Drug Resist."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e29314","DOI":"10.5812\/jjm.29314","article-title":"Primer Evaluation for PCR and its Application for Detection of Carbapenemases in Enterobacteriaceae","volume":"9","author":"Mlynarcik","year":"2016","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.1128\/JCM.41.9.4264-4269.2003","article-title":"Multiple CTX-M-Type extended-spectrum b-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy","volume":"41","author":"Pagani","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1128\/AAC.50.4.1442-1448.2006","article-title":"Genetic structures at the origin of acquisition and expression of the carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) in Acinetobacter baumannii","volume":"50","author":"Poirel","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications","volume":"3","author":"Jolley","year":"2018","journal-title":"Wellcome Open Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1080\/14756366.2017.1344235","article-title":"Beta-lactamase database (BLDB)\u2014Structure and function","volume":"32","author":"Naas","year":"2017","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_19","unstructured":"Li, R.W. (2011). Automatic annotation of microbial genomes and metagenomic sequences. Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies, Nova Science Publishers."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.ijantimicag.2006.12.012","article-title":"Pandrug-resistant Gram-negative bacteria: The dawn of the post-antibiotic era?","volume":"29","author":"Falagas","year":"2007","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"523","DOI":"10.3201\/eid1803.111330","article-title":"Carbapenemase-producing Acinetobacter spp. in cattle, France","volume":"18","author":"Poirel","year":"2012","journal-title":"Emerg. Infect. Dis. J."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wang, Y., Wu, C., Zhang, Q., Qi, J., Liu, H., Wang, Y., He, T., Ma, L., Lai, J., and Shen, Z. (2012). Identification of New Delhi metallo-beta-lactamase 1 in Acinetobacter lwoffii of food animal origin. PLoS ONE, 7.","DOI":"10.1371\/annotation\/e33066ea-f500-4d34-8981-7cfd68497e73"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"165","DOI":"10.2754\/avb201887020165","article-title":"Occurrence of bacteria with a dangerous extent of antibiotic resistance in poultry in the Central Region of Moravia","volume":"87","author":"Bardon","year":"2018","journal-title":"Acta Vet. Brno"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9254907","DOI":"10.1155\/2016\/9254907","article-title":"The First Outbreak Caused by Acinetobacter baumannii ST208 and ST195 in China","volume":"2016","author":"Qu","year":"2016","journal-title":"Biomed. Res. Int"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1093\/jac\/dkv072","article-title":"Investigation of a possible outbreak of carbapenem-resistant Acinetobacter baumannii in Odense, Denmark using PFGE, MLST and whole-genome-based SNPs","volume":"70","author":"Hammerum","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1099\/jmm.0.028340-0","article-title":"Emergence of OXA-carbapenemase- and 16S rRNA methylase-producing international clones of Acinetobacter baumannii in Norway","volume":"60","author":"Karah","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1128\/AAC.50.5.1633-1641.2006","article-title":"Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates","volume":"50","author":"Quale","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1111\/j.1469-0691.2006.01456.x","article-title":"Carbapenem resistance in Acinetobacter baumannii: Mechanisms and epidemiology","volume":"12","author":"Poirel","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"501","DOI":"10.2217\/17460913.2.5.501","article-title":"Carbapenemases: Molecular diversity and clinical consequences","volume":"2","author":"Poirel","year":"2007","journal-title":"Future Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/jac\/dkh251","article-title":"Evolutionary mapping of the SHV beta-lactamase and evidence for two separate IS26-dependent blaSHV mobilization events from the Klebsiella pneumoniae chromosome","volume":"54","author":"Ford","year":"2004","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_31","first-page":"77","article-title":"Extended-spectrum resistance to beta-lactams\/beta-lactamase inhibitors (ESRI) evolved from low-level resistant Escherichia coli","volume":"75","author":"Bonnin","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e22976","DOI":"10.1002\/jcla.22976","article-title":"Mutation of CarO participates in drug resistance in imipenem-resistant Acinetobacter baumannii","volume":"33","author":"Zhu","year":"2019","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"137","DOI":"10.4103\/ijmm.IJMM_15_220","article-title":"Carbapenem nonsusceptibility with modified OprD in clinical isolates of Pseudomonas aeruginosa from India","volume":"35","author":"Choudhury","year":"2017","journal-title":"Indian J. Med. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.eimc.2015.12.014","article-title":"Characterisation of carbapenem-resistance mechanisms in clinical Pseudomonas aeruginosa isolates recovered in a Spanish hospital","volume":"35","author":"Estepa","year":"2017","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1093\/jac\/dkl509","article-title":"Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii","volume":"59","author":"Vila","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1128\/AAC.02556-12","article-title":"RND-type efflux pumps in multidrug-resistant clinical isolates of Acinetobacter baumannii: Major role for AdeABC overexpression and AdeRS mutations","volume":"57","author":"Yoon","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1128\/AAC.01388-10","article-title":"Efflux-mediated antibiotic resistance in Acinetobacter spp.","volume":"55","author":"Coyne","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1128\/AAC.00732-07","article-title":"AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii","volume":"52","author":"Magnet","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4389","DOI":"10.1128\/AAC.00155-10","article-title":"Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii","volume":"54","author":"Coyne","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5260","DOI":"10.1128\/AAC.00562-15","article-title":"Mobile genetic elements related to the diffusion of plasmid-mediated AmpC beta-lactamases or carbapenemases from Enterobacteriaceae: Findings from a multicenter study in Spain","volume":"59","author":"Zamorano","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1128\/AAC.48.8.2845-2852.2004","article-title":"DNA sequence analysis of regions surrounding blaCMY-2 from multiple Salmonella plasmid backbones","volume":"48","author":"Giles","year":"2004","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-1729\/11\/10\/1079\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:12:26Z","timestamp":1760166746000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-1729\/11\/10\/1079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,13]]},"references-count":41,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["life11101079"],"URL":"https:\/\/doi.org\/10.3390\/life11101079","relation":{},"ISSN":["2075-1729"],"issn-type":[{"type":"electronic","value":"2075-1729"}],"subject":[],"published":{"date-parts":[[2021,10,13]]}}}