{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:59:43Z","timestamp":1773298783879,"version":"3.50.1"},"reference-count":151,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,13]],"date-time":"2021-11-13T00:00:00Z","timestamp":1636761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Life"],"abstract":"<jats:p>There is a unique microbial community in the female lower genital tract known as the vaginal microbiota, which varies in composition and density and provides significant benefits during pregnancy, reproductive cyclicity, healthy newborn delivery, protection from preterm birth, infections such as UTIs, bacterial vaginosis, and so on, and improves the efficacy of treatments for vaginal cancers. Methods: It is necessary to know how the vaginal microbiome is composed in order to make an accurate diagnosis of the diseases listed above. A microbiome\u2019s members are difficult to classify, and the way microbial communities function and influence host\u2013pathogen interactions are difficult to understand. More and more metagenomic studies are able to unravel such complexities due to advances in high-throughput sequencing and bioinformatics. When it comes to vaginal microbiota research, we\u2019ll be looking at the use of modern techniques and strategies that can be used to investigate variations in vaginal microbiota in order to detect diseases earlier, better treat vaginal disorders, and boost women\u2019s health. Discussion: The discussed techniques and strategies may improve the treatment of vaginal disorders and may be beneficial for women\u2019s overall health.<\/jats:p>","DOI":"10.3390\/life11111229","type":"journal-article","created":{"date-parts":[[2021,11,14]],"date-time":"2021-11-14T20:51:53Z","timestamp":1636923113000},"page":"1229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["An Insight into Vaginal Microbiome Techniques"],"prefix":"10.3390","volume":"11","author":[{"given":"Mahima","family":"Sharma","sequence":"first","affiliation":[{"name":"School of Biotechnology, Shri Mata Vaishno Devi University, Katra 182320, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chitrakshi","family":"Chopra","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Shri Mata Vaishno Devi University, Katra 182320, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malvika","family":"Mehta","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Shri Mata Vaishno Devi University, Katra 182320, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6314-4613","authenticated-orcid":false,"given":"Varun","family":"Sharma","sequence":"additional","affiliation":[{"name":"Ancient DNA Laboratory, Birbal Sahni Institute of Palaeosciences, Lucknow 226007, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0880-5394","authenticated-orcid":false,"given":"Sharada","family":"Mallubhotla","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Shri Mata Vaishno Devi University, Katra 182320, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3693-8597","authenticated-orcid":false,"given":"Srinivas","family":"Sistla","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University, New York, NY 11790, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyothi C.","family":"Sistla","sequence":"additional","affiliation":[{"name":"Institute of Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, VA 23219, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indu","family":"Bhushan","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Shri Mata Vaishno Devi University, Katra 182320, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/nature06244","article-title":"The human microbiome project","volume":"449","author":"Turnbaugh","year":"2007","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S48","DOI":"10.1016\/j.jrhm.2016.11.007","article-title":"Female reproductive tract microbiome in gynecological health and problems","volume":"2","author":"Haldar","year":"2016","journal-title":"J. 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